期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 77, 期 5, 页码 966-970出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.120921
关键词
cyanobacteria; lactic acid; genetic engineering; lactate dehydrogenase; Synechocystis sp.
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Core Research for Evolution Science and Technology (CREST), Japan
Metabolic pathway engineering of cyanobacteria for the production of industrially important chemicals from atmospheric CO2 has generated interest recently. Here, we engineered Synechocystis sp. PCC 6803 to produce lactic acid using a lactate dehydrogenase (ldh) gene from various lactic acid-producing bacteria, Lactococcus lactis (ldhB and ldhX), Lactobacillus plantarum (ldhL and ldh), and Lactobacillus rhamnosus (ldhL). The lactic acid was secreted outside the cell using a transporter (lldp) gene from L. plazztarum. Expression of each ldh in Synechocystis sp. PCC6803 was ascertained by reverse-transcriptase polymerase chain reaction. Five transformants led to the production of L-lactic acid. Coexpression of lldp with ldhB from L. plantarum or ldhL from L. rhamnosus led to the secretion of lactic acid into the medium at concentration of 0.17 +/- 0.02 or 0.14 +/- 0.02 mm after 18 d of cultivation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据