期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 76, 期 7, 页码 1422-1424出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.120366
关键词
Corynebacterium glutamicum; glutamate overproduction; mechanosensitive channel; patch-clamp method; exporter
类别
资金
- Research Institute for Science and Technology of Tokyo Denki University [Zb10-01]
- Grants-in-Aid for Scientific Research [24580124] Funding Source: KAKEN
The NCgl1221 gene, which encodes a mechanosensitive channel, has been reported to be critically involved in glutamate (Glu) overproduction by Corynebacterium glutamicum, but direct evidence of Glu excretion through this channel has not yet been provided. In this study, by electrophysiological methods, we found direct evidence of Glu excretion through this channel by passive diffusion. We found that the introduction into Phe-producing Escherichia coli of mutant NCgl1221 genes that induce Glu overproduction by C. glutamicum improved productivity. This suggests a low-substrate preference of this channel, indicates its potential as a versatile exporter, and more broadly, indicates the potential of exporter engineering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据