期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 6, 页码 1290-1292出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100027
关键词
dehalogenase; metagenome; tetrachloroethene; tetrachloroethylene; DnaK
Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.
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