期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 7, 页码 1435-1440出版社
OXFORD UNIV PRESS
DOI: 10.1271/bbb.100111
关键词
noncoding RNA; RNAi; trans-acting siRNA (tasiRNA); robustness; microRNA (miRNA)
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grant-in-Aid for Exploratory Research [20658079]
- Japan Science Society [21-415]
- Grants-in-Aid for Scientific Research [20658079] Funding Source: KAKEN
To endure considerable fluctuations in temperature, plants need precise regulation of temperature-controlled gene expression. In this study, the involvement of TAS1 trans-acting siRNA (tasiRNA) in temperature-controlled gene expression was examined in Arabidopsis. The accumulation of TAS1 tasiRNA was downregulated at 4 degrees C. Concomitant with the reduction of TAS1 tasiRNA-mediated cleavage, expression of At1g51670, a target of TAS1 tasiRNA, was upregulated at 4 degrees C in the wild type but not in a dicer-like enzyme (DCL) 4 mutant (dcl4-2), which is impaired in tasiRNA biogenesis. The expression of At4g29760 and of At5g18040, further TAS1 tasiRNA targets, was upregulated both in the wild type and in dcl4-2 at 4 degrees C. However, after shifting the temperature to 22 degrees C, low-temperature-induced expression of At4g29760 rapidly dropped in the wild type, but not in dcl4-2. Thus TAS1 tasiRNA acted as a sweeper for the clearance of excess amounts of At4g29760 transcripts. Our data suggest that differential regulation of TAS1 tasiRNA targets is involved in temperature-controlled gene expression.
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