期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 7, 页码 1386-1390出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100060
关键词
human rotavirus; diarrhea; bovine milk; lactophorin; PAS6/7
类别
资金
- Japan Society for the Promotion of Science (JSPS) [09J06778, 19580309]
- Promotion of Basic and Applied Research
- Grants-in-Aid for Scientific Research [19580309, 09J06778] Funding Source: KAKEN
Rotaviruses are the leading cause of severe dehydrating diarrhea in children worldwide. We have found that high-M-r glycoprotein fraction (F1) from cow's milk whey has potent inhibitory activity against human rotavirus (HRV) in cell culture. The present study was undertaken to identify and characterize the components responsible for this inhibitory activity. F1 was initially heated at 95 degrees C for 30 min, rendering milk antibodies inert, subjected to ammonium sulfate fractionation, and then resolved by two-dimensional polyacrylamide gel electrophoresis. After electroelution, we found that a heat-stable milk protein lactophorin C-terminal fragment (LP16) and bovine milk fat globule membrane protein PAS6/7 strongly inhibited the replication of HRV MO strains in MA 104 cells. Furthermore, we found that prophylactic oral administration of F1 once before inoculation of the HRV MO strain obviously prevented the development of diarrhea in vivo. These non-immunoglobulin components are a promising candidate for a prophylactic food additive against HRV infection.
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