期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 72, 期 12, 页码 3174-3179出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.80361
关键词
fatty acid desaturase; Saccharomyces kluyveri; polyunsaturated fatty acid
类别
资金
- Japan Society for the Promotion of Science [18880011]
- Grants-in-Aid for Scientific Research [18880011] Funding Source: KAKEN
Delta 12 and omega 3 fatty acid desaturases are key enzymes in the synthesis of polyunsaturated fatty acids (PUFAs), which are important constituents of membrane glycerolipids and also precursors to signaling molecules in many organisms. In this study, we determined the substrate specificity and regioselectivity of the Delta 12 and omega 3 fatty acid desaturases from Saccharomyces kluyveri (Sk-FAD2 and Sk-FAD3). Based on heterologous expression in Saccharomyces cerevisiae, it was found that Sk-FAD2 converted C16-20 monounsaturated fatty acids to diunsaturated fatty acids by the introduction of a second double bond at the nu + 3 position, while Sk-FAD3 recognized the omega 3 position of C18 and C20. Furthermore, fatty acid analysis of major phospholipids suggested that Sk-FAD2 and Sk-FAD3 have no strong substrate specificity toward the lipid polar head group or the sn-positions of fatty acyl groups in phospholipids.
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