期刊
BIORESOURCE TECHNOLOGY
卷 268, 期 -, 页码 583-591出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.08.038
关键词
Microalgae; Biodiesel; Chlorella; Wet microalgal biomass; Two-step enzymatic process
资金
- Public Science and Technology Research Funds Projects of Ocean [201505032]
- China Postdoctoral Science Foundation [2017M620530]
- National Natural Science Foundation of China [31471717]
- Special National Key Research and Development Plan [2016YFD0400204]
In this study, a novel two-step enzymatic process was firstly established to produce microalgae biodiesel using wet Chlorella biomass. In the first hydrolysis step, to reduce energy consumption and effectively disrupt microalgal cell wall, among cellulase, hemicellulase, papain, lysozyme and pectinase, the highest hydrolysis efficiency (67.52%) was obtained by cellulase at pH 5.0 with enzyme dosage of 200 U/g dry biomass at 40 degrees C for 12 h. In the second transesterification step, compared with liquid CAL-A/B from Candida antarctica and PLA from Aspergillus oryzae, liquid lipase TL from Thermomyces lanuginosus achieved the highest biodiesel conversion at 81.15:1 (v/w) ethanol/g TFAs ratio in 78-83% water content with 100 PLU/g TFAs lipase loading at 25 degrees C for 48 h. Moreover, similar results were obtained with three Chlorella species by this process. Overall, this two-step enzymatic process was a green, low-energy and efficient method for cost-effective biodiesel production using wet microalgal biomass.
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