期刊
BIORESOURCE TECHNOLOGY
卷 169, 期 -, 页码 559-565出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.06.056
关键词
Ca(OH)(2); D-Lactic acid; Escherichia coli; Neutralization; PLA
资金
- China National Natural Science Foundation [NSFC31070094]
- Hubei Provincial Science Foundation [2011CDA008]
- Chutian Scholar Program, Hubei University of Technology, P.R. China
- Northern Illinois University, USA
In this study, a genetically engineered Escherichia coli strain, HBUT-D (Delta pflB Delta pta Delta frdABCD Delta adhE Delta ald Delta cscR), was initially evaluated on a laboratory scale (7 L) in a glucose (130 g L-1) mineral salts medium for D-lactic acid fermentation using 6 N KOH, Ca(OH)(2) or NH4OH as the neutralizing agent. Fermentations neutralized by Ca(OH)(2) achieved a volumetric productivity of 6.35 g L-1 h(-1), tripling that achieved by KOH (1.71 g L-1 h(-1)) and NH4OH (1.5 g L-1 h(-1)). The facilitative effect of Ca(OH)(2) neutralization was then demonstrated on a pilot scale (6 ton vessel, 130 kg glucose ton(-1)), resulting in a volumetric productivity of 6 kg ton(-1) h(-1), a titer of 126 kg ton(-1), a yield of 97%, and an optical purity of 99.5%. These results demonstrated that E. coli HBUT-D is a promising strain for large scale D-lactic acid fermentation using mineral salts medium and Ca(OH)(2) for neutralization. (C) 2014 Elsevier Ltd. All rights reserved.
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