期刊
BIORESOURCE TECHNOLOGY
卷 171, 期 -, 页码 500-505出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.08.112
关键词
Lipid; Chlorella sp.; Coal-fired flue-gas; Fed-batch; Mixotrophic culture
资金
- Korea Institute of Energy Technology Evaluation and Planning (KETEP)
- Ministry of Knowledge Economy (MKE) of Korea [2012-T-100201516]
- Advanced Biomass R&D Center (ABC) of the Global Frontier Project - Ministry of Science, ICT and Future Planning [ABC-2012M3A6A205388]
Industrial CO2-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561 mg/L d) and fatty-acid methyl-ester (168 mg/L d) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers. (C) 2014 Elsevier Ltd. All rights reserved.
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