期刊
BIORESOURCE TECHNOLOGY
卷 151, 期 -, 页码 347-354出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.10.064
关键词
FT-IR; Microalgae; Cell composition; Strain screening; Physiological status
资金
- Hundred Talent Program of the Chinese Academy of Sciences [A1097]
- Hi-Tech Research and Development Program of China [2012AA052101]
- National Key Basic Research Program of China [2011CB200903]
- Liaoning Provincial Natural Science Foundation [2012010263]
- Knowledge Innovation Programs of Dalian Institute of Chemical Physics, Chinese Academy of Sciences [K2010A13]
Fourier transform infrared spectroscopy (FT-IR) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga Isochrysis zhangjiangensis during algal cultivation. The content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of FT-IR method. For algal screening, the band absorption ratios of lipid/amide I and carbo/amide I from FT-IR measurements allowed for the selection of Isochrysis sp. and Tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. The cell composition dynamics oft zhangjiangensis measured by FT-IR revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. The carbo/amide I band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in T. subcordiformis. FT-IR serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell. (C) 2013 Elsevier Ltd. All rights reserved.
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