期刊
BIORESOURCE TECHNOLOGY
卷 135, 期 -, 页码 459-463出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.042
关键词
Acid tolerance; Error-prone amplification; Whole genome; Lactobacillus pentosus; Lactic acid
资金
- Science and Engineering Research Council (SERC)
- Agency for Science, Technology and Research (A*STAR)
- Value-Added Chemicals from Linocellulose (VACL) [0921590133, ICES/10-474A01, NUS/279-000-309-305]
Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20 g/L of glucose to produce lactic acid at a yield of 95% in 1 L MRS medium at pH 3.8 within 36 h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms. (C) 2012 Elsevier Ltd. All rights reserved.
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