期刊
BIORESOURCE TECHNOLOGY
卷 150, 期 -, 页码 259-265出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.10.008
关键词
Acetamiprid; Co-metabolism; Response surface methodology; Kinetics
资金
- National Natural Science Foundation of China [31100083]
An AAP-degrading bacterium, AAP-7, was isolated from AAP-polluted soil. MP-7 was identified as Pseudoxanthomonas sp. on the basis of the comparative analysis of 165 rDNA sequences. The strain was able to transformate more than 80% AAP by means of co-metabolism and degraded AAP via hydrolysis or demethylation to form (E)-3-(((6-chloropyridin-3yl)methyl)(methyl)amino)acrylonitrile and N((6-chloropyridin-3yl)methyl)-N-methylprop-1-en-2-amine, both of which transformed into ultimate product, which was 1-(6-chloropyridin-3yl)-N-methylmethanamine. A novel degradation pathway was proposed based on these metabolites. AAP could be transformed with a maximum specific degradation rate, half-saturation constant and inhibit constant of 1.775/36 h. 175.3 mg L-1, and 396.5 mg L-1, respectively, which proved that the degradation rate of AAP could be restrained at high AAP concentration. This paper highlights a significant potential use of co-metabolic cultures of microbial cells for the cleanup of AAP-contaminated soil. (C) 2013 Elsevier Ltd. All rights reserved.
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