期刊
BIORESOURCE TECHNOLOGY
卷 146, 期 -, 页码 27-34出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.07.028
关键词
Polychlorinated biphenyls; Biphenyl degradation; Mixed-culture; Uncultured bacteria; Resuscitation-promoting factor
资金
- National Natural Science Foundation of China [41271334, 81001475]
- National High Technology Research and Development Program of China [2009AA063104, 2012AA06A203]
- Zhejiang Provincial Natural Science Foundation of China [LR12D01001]
- Science and Technology Development Foundation of Hangzhou [20101131N05]
SRpf, culture supernatants from Micrococcus luteus containing the resuscitation-promoting factor (Rpf), was used to enhance the biphenyl-degrading capability of potential microorganisms. The obtained results suggest that the enrichment culture produced by the addition of SRpf (enrichment culture treatment group, ECT) enhanced the biphenyl degradation efficiency, cell growth and bacterial diversity significantly. Biphenyl at concentration of 1500 mg/L was almost completely degraded in 24 h using SRpf at a dosage of 15% (v/v). Six strains unique to the ECT were isolated in pure cultures. This study provides a new insight into bacterial degradation of biphenyl for PCBs-bioremediation, and could be developed as a novel efficient method for obtaining highly desirable pollutant-degrading microorganisms. (c) 2013 Elsevier Ltd. All rights reserved.
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