Review
Biotechnology & Applied Microbiology
Govinda R. Navale, Mahesh S. Dharne, Sandip S. Shinde
Summary: Isoprenoids, also known as terpenoids, are diverse organic compounds found abundantly in nature, with various applications in pharmaceutical, nutraceutical, and chemical industries. Recent advancements in metabolic engineering have enabled the production of isoprenoids in heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Challenges and strategies for scale-up and engineering of isoprenoids in heterologous host systems have been discussed in detail.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Michael J. Volk, Vinh G. Tran, Shih- Tan, Shekhar Mishra, Zia Fatma, Aashutosh Boob, Hongxiang Li, Pu Xue, Teresa A. Martin, Huimin Zhao
Summary: Metabolic engineering aims to improve the production of economically valuable molecules through genetic manipulation. Advancements in this discipline have led to industrial-level molecule production benefiting various industries. This review provides insights into the steps required for a successful metabolic engineering campaign, highlights major applications, and discusses future challenges.
Article
Agriculture, Multidisciplinary
Chenyao Zhou, Mingjie Li, Surui Lu, Yanfei Cheng, Xuena Guo, Xiaoxian He, Zhaoyue Wang, Xiu-Ping He
Summary: This study utilized the Escherichia coli MAR operator cis-element marO to engineer promoters in Saccharomyces cerevisiae for metabolic flux control, resulting in increased production of squalene and lanosterol. The engineered promoters using marO were effective in reducing the accumulation of other metabolites, leading to higher yields of the target products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Vinh G. Tran, Somesh Mishra, Sarang S. Bhagwat, Saman Shafaei, Yihui Shen, Jayne L. Allen, Benjamin A. Crosly, Shih-I Tan, Zia Fatma, Joshua D. Rabinowitz, Jeremy S. Guest, Vijay Singh, Huimin Zhao
Summary: In this study, we successfully engineered yeast to produce succinic acid at high efficiency under low pH conditions. The high titers of succinic acid obtained in the experiments, as well as the economic and environmental benefits demonstrated in the techno-economic and life cycle assessments, indicate the potential of scaling up this process for industrial applications.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Vinh G. Tran, Somesh Mishra, Sarang S. Bhagwat, Saman Shafaei, Yihui Shen, Jayne L. Allen, Benjamin A. Crosly, Shih- Tan, Zia Fatma, Joshua D. Rabinowitz, Jeremy S. Guest, Vijay Singh, Huimin Zhao
Summary: This study presents a metabolic engineering approach to enhance the production of succinic acid (SA) by acid-tolerant yeast, achieving high titers at low pH conditions. The pilot-scale fermentation using sugarcane juice medium resulted in a SA titer of 63.1g/L with a high crystallization yield. Techno-economic analysis and life cycle assessment indicate the financial viability and significant reduction in greenhouse gas emissions of the proposed low-pH SA production process.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Taehee Han, Sang Yup Lee
Summary: The biosynthesis of Valerolactam (VL) was achieved by metabolically engineered Corynebacterium glutamicum, which overproduces L-lysine. This provides a green and sustainable method for the production of precursor chemicals for nylon-5 and nylon 6,5.
METABOLIC ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Jinhui Niu, Zhitao Mao, Yufeng Mao, Ke Wu, Zhenkun Shi, Qianqian Yuan, Jingyi Cai, Hongwu Ma
Summary: The genome-scale enzyme-constrained model (ecCGL1) improves the prediction of phenotypes and simulates overflow metabolism in Corynebacterium glutamicum, while also recapitulating the trade-off between biomass yield and enzyme usage efficiency.
Review
Chemistry, Multidisciplinary
Bruna de Falco, Francesco Giannino, Fabrizio Carteni, Stefano Mazzoleni, Dong-Hyun Kim
Summary: This review outlines the experimental procedures used in C-13-MFA, including cell culture preparation, labelled tracer preparation, metabolite extraction and analysis. The limitations and advantages of nuclear magnetic resonance and mass spectrometry techniques in carbon labelled experiments are discussed. Additionally, successful computational modelling approaches in flux analysis and the main application areas in metabolic engineering are summarized.
Review
Chemistry, Multidisciplinary
Kei-Anne Baritugo, Jina Son, Yu Jung Sohn, Hee Taek Kim, Jeong Chan Joo, Jong-il Choi, Si Jae Park
Summary: This study discusses the recent development of Corynebacterium glutamicum in producing platform chemicals from renewable resources, utilizing synthetic biology tools and techniques to transform it into a versatile microbial cell factory.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Review
Microbiology
Jay Keasling, Hector Garcia Martin, Taek Soon Lee, Aindrila Mukhopadhyay, Steven W. Singer, Eric Sundstrom
Summary: Concerns over climate change have led to a rethinking of transportation infrastructure, with biofuels emerging as a possible alternative to fossil fuels. However, limited uptake of biofuels is attributed to cost and quantity limitations.
NATURE REVIEWS MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Karolina Anna Mielko, Slawomir Jan Jablonski, Marcin Lukaszewicz, Piotr Mlynarz
Summary: Metabolic analyses of six bacterial strains were conducted with three cell disintegration methods, showing significant influence on metabolite concentrations and differences among strains. NMR could be useful for bacterial strain identification, and regardless of the disintegration method, strain groups could be identified through multivariate data analysis.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Xi Sun, Yufeng Mao, Jiahao Luo, Pi Liu, Meiru Jiang, Guimei He, Zhidan Zhang, Qichen Cao, Jie Shen, Hongwu Ma, Tao Chen, Zhiwen Wang
Summary: A novel xylose regulatory mechanism mediated by the transcription factor IpsA was revealed, and a synergistic effect on carbon metabolism and energy supply was found to endow C. glutamicum with the efficient xylose utilization and rapid growth phenotype. This study not only provides promising C. glutamicum chassis strains for lignocellulosic biorefinery, but also enriches the understanding of xylose regulatory mechanism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Xinfang Song, Mingyu Dong, Min Liu
Summary: The article introduces a new metabolic pathway design tool called PyMiner, which can effectively simplify metabolic networks, improve search efficiency, and demonstrate excellent performance.
Article
Chemistry, Analytical
Qichen Cao, Manman Han, Zuoqing Zhang, Chang Yu, Lida Xu, Tuo Shi, Ping Zheng, Jibin Sun
Summary: With fast growth, synthetic biology enables efficient production of high commercial value products. Understanding the protein regulatory network is key to building cell factories for target hyperproduction. A novel metabolic labeling-based absolute quantification approach (nMAQ) is proposed, which utilizes prequantified reference proteins as internal standards to quantify target proteins. nMAQ is cost-effective and has been benchmarked for quantitative performance.
ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Zijian Liang, Hang Zhi, Zhongxiang Fang, Pangzhen Zhang
Summary: This review explores the significance of terpenes in grapes and wines, as well as the application of microbial engineering platforms such as yeast in terpene production. Additionally, the potential applications of terpene products in the food and beverage industry are discussed.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Fabia Weiland, Michael Kohlstedt, Christoph Wittmann
Summary: Lignin, an important structural component of plants, is considered as an under-utilized and promising feedstock due to its abundance and richness in aromatics. Microbes have evolved enzymes and pathways to break down lignin and metabolize its aromatic components. Systems metabolic engineering offers a sustainable approach to upgrade lignin into a variety of valuable products.
METABOLIC ENGINEERING
(2022)
Correction
Biotechnology & Applied Microbiology
Dominik Weixler, Max Berghoff, Kirill V. Ovchinnikov, Sebastian Reich, Oliver Goldbeck, Gerd M. Seibold, Christoph Wittmann, Nadav S. Bar, Bernhard J. Eikmanns, Dzung B. Diep, Christian U. Riedel
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Dominik Weixler, Max Berghoff, Kirill Ovchinnikov, Sebastian Reich, Oliver Goldbeck, Gerd M. Seibold, Christoph Wittmann, Nadav S. Bar, Bernhard J. Eikmanns, Dzung B. Diep, Christian U. Riedel
Summary: We have successfully established a two-step process for recombinant production of active nisin with C. glutamicum. This allows for the production of a bacteriocin with complex posttranslational modifications using C. glutamicum, expanding the range of bioactive compounds that can be produced. Our results provide a foundation for further research to optimize product yields, transfer production to sustainable substrates, and purify pharmaceutical grade nisin.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Muzi Tangyu, Michel Fritz, Lijuan Ye, Rosa Aragao Boerner, Delphine Morin-Rivron, Esther Campos-Gimenez, Christoph J. Bolten, Biljana Bogicevic, Christoph Wittmann
Summary: This study evaluated the potential of microbes to enhance the nutritional value and flavor of sunflower seed milk. By co-fermenting the milk with two food-grade microbes, vitamin B-12 and other beneficial components were significantly increased, resulting in an attractive and clean-label product.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Dennis Schulze, Michael Kohlstedt, Judith Becker, Edern Cahoreau, Lindsay Peyriga, Alexander Makowka, Sarah Hildebrandt, Kirstin Gutekunst, Jean-Charles Portais, Christoph Wittmann
MICROBIAL CELL FACTORIES
(2022)
Article
Oncology
Charlotte Dahlem, Shilpee Chanda, Jan Hemmer, Hanna S. Schymik, Michael Kohlstedt, Christoph Wittmann, Alexandra K. Kiemer
Summary: This study investigates the anti-cancer activities of the purple compound violacein and highlights its inhibitory effect on tumor cell proliferation and migration. The study also demonstrates the association between violacein-induced tumor cell death and inflammatory activation of macrophages. The results suggest that violacein may induce immunogenic cell death.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Michael Kohlstedt, Anna Weimer, Fabia Weiland, Jessica Stolzenberger, Mirjam Selzer, Miguel Sanz, Laurenz Kramps, Christoph Wittmann
Summary: In this study, a cascaded value chain for the production of green PET from lignin was demonstrated. By fermenting lignin-based aromatics, PET monomers were synthesized, providing a sustainable alternative to fossil-based PET production.
METABOLIC ENGINEERING
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Yu Wang, Ping Zheng, Zhenghong Xu, Akihiko Kondo, Christoph Wittmann, Volker F. Wendisch
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Christina Rohles, Sarah Pauli, Gideon Giesselmann, Michael Kohlstedt, Judith Becker, Christoph Wittmann
Summary: Through optimization of C. glutamicum cell factories, high titer, yield, and productivity of 5-aminovalerate have been achieved, laying the foundation for industrial production of this compound.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Fabia Weiland, Nadja Barton, Michael Kohlstedt, Judith Becker, Christoph Wittmann
Summary: Lignin, a renewable resource, is mostly burned in lignocellulosic processing facilities due to a lack of value-added alternatives. In this study, C. glutamicum was metabolically engineered to produce cis, cis-muconate (MA) from key aromatics, such as vanillin and vanillate. Through the implementation of synthetic pathway modules and the identification of efficient enzymes, the final strain MA-9 was able to produce bio-based MA with high selectivity.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Lukas Jungmann, Sarah Lisa Hoffmann, Caroline Lang, Raphaela De Agazio, Judith Becker, Michael Kohlstedt, Christoph Wittmann
Summary: This study demonstrates the production of hydroxyectoine through metabolic engineering, using a genetically modified strain of C. glutamicum. The optimized strain achieved high yields of hydroxyectoine, showing potential for its use in the cosmetics and pharmaceutical industries as an active ingredient.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Sarah Pauli, Michael Kohlstedt, Jessica Lamber, Fabia Weiland, Judith Becker, Christoph Wittmann
Summary: The nonproteinogenic cyclic metabolite L-pipecolic acid is a chiral precursor for various commercial drugs. It has multiple uses in the pharmaceutical, medical, cosmetic, and agrochemical markets. The production of L-pipecolic acid from glucose using Corynebacterium glutamicum has been improved through systems metabolic engineering. The advanced mutant strain achieved a high titer of 93 g L-1, approaching the level of biotransformation from L-lysine.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Jens Christmann, Peng Cao, Judith Becker, Christian K. K. Desiderato, Oliver Goldbeck, Christian U. U. Riedel, Michael Kohlstedt, Christoph Wittmann
Summary: This study optimized the production process of pediocin PA-1 and found that an acidic pH of 5.7, microaerobic conditions, and the addition of high levels of CaCl2 significantly increased its production. By applying the optimized conditions, the IPTG-inducible producer C. glutamicum CR099 pXMJ19 P-tac pedACD(Cg) achieved a pediocin titer of 66 mg L-1, while the novel constitutive strain P-tuf pedACD(Cg) successfully produced pediocin without the need for IPTG.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Sofija Jovanovic Gasovic, Demian Dietrich, Lars Glaeser, Peng Cao, Michael Kohlstedt, Christoph Wittmann
Summary: This study reveals the cellular dynamics of DHA production in the oleaginous yeast Y. lipolytica Af4 by using transcriptomics, metabolomics, and 13C-based metabolic pathway profiling. It is found that supplementation with ketogenic amino acids such as L-lysine can increase DHA production.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Laura Pause, Anna Weimer, Nicolas T. Wirth, Anh Vu Nguyen, Claudius Lenz, Michael Kohlstedt, Christoph Wittmann, Pablo I. Nikel, Bin Lai, Jens O. Kroemer
Summary: In a bio-electrochemical system, the obligate aerobe Pseudomonas putida is able to survive anaerobically and produce chemicals via oxidative pathways. The utilization of carbon in the cytoplasm is not limited by the stoichiometry of the sugar uptake routes, but could be affected by energy constraints or an oxygen-dependent regulatory mechanism.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
