期刊
BIORESOURCE TECHNOLOGY
卷 129, 期 -, 页码 321-328出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.11.090
关键词
Butanol tolerance; Clostridium acetobutylicum; Immobilization; Metabolic flux; NAD(P)H
资金
- National Outstanding Youth Foundation of China [21025625]
- National High-Tech Research and Development Program of China (863) [2012AA021200]
- National Basic Research Program of China (973) [2011CBA00806]
- National Natural Science Foundation of China, Youth Program [21106070]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1066]
- Jiangsu Provincial Natural Science Foundation of China [SBK 201150207]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
The objective of this study was to improve butanol yield and productivity by redox modulation and immobilization of Clostridium acetobutylicum B3 cells. Stoichiometric network analysis revealed that NAD(P)H that had escaped from the fermentation as H-2 limited the butanol yield and led to the accumulation of oxidation byproducts, e.g., acetone. Methyl viologen was used as an electron carrier to divert the electron flow away from H-2 production and to reinforce the NAD(P)H supply. Butanol yield was increased by 37.8% with severely diminished acetone production. Immobilization of the cells by adsorption onto a fibrous matrix improved their butanol tolerance and production rate. An average of 15.6 g/L butanol was achieved within 12 h with a solvent productivity of 1.88 g/L/h in repeated batch fermentation. To our knowledge, this is the highest solvent productivity with a relatively high butanol titer produced by a Clostridium strain in batch fermentation. (C) 2012 Elsevier Ltd. All rights reserved.
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