Article
Biotechnology & Applied Microbiology
Maresa Bussa, Cordt Zollfrank, Hubert Roeder
Summary: This paper presents a parameterised life cycle assessment approach to determine threshold values for input streams in microalgal biorefinery production. The study identifies membrane technologies as more effective for protein and polysaccharide extraction and supercritical carbon extraction as promising for lipid recovery. Further improvements are needed for energy demand in drying processes and protein recovery yields in membrane technologies.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Biochemistry & Molecular Biology
Zichao Wang, Yi Zheng, Ziru Lai, Xilei Hu, Lu Wang, Xueqin Wang, Zhitao Li, Minjie Gao, Yahui Yang, Qi Wang, Na Li
Summary: Polysaccharides are widely used in pharmaceutical and food industries due to their diverse bioactivity, high safety, and minimal side effects. The ability to quickly produce, screen, and synthesize bioactive polysaccharides remains a limiting factor. This research reviews the effects of monosaccharide composition and proportion on various bioactivities of polysaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Chemistry, Applied
Jingyi Wang, Zuwei Yu, Wenjuan Wu, Shumin He, Bijun Xie, Muci Wu, Zhida Sun
Summary: The study reveals the non-covalent interactions between ECG and CMPN, including hydrogen bonding, hydrophobic and electrostatic interactions, leading to changes in physicochemical properties and enhanced antibacterial activity. Analysis through 2D-FT-IR and NMR shows that COO- and C=O are key functional groups affecting the interactions between polysaccharides and flavanols.
CARBOHYDRATE POLYMERS
(2022)
Review
Polymer Science
Svetlana R. Derkach, Nikolay G. Voron'ko, Yulia A. Kuchina
Summary: This review summarizes recent advances in the study of polysaccharide-gelatin complexes, focusing on data obtained by spectroscopic methods. The discussion highlights the complexing polysaccharides of marine origin, such as sodium alginate, kappa-carrageenan, and chitosan. The prospects for creating polysaccharide-gelatin complexes with desired physicochemical properties are outlined.
Article
Energy & Fuels
Marta Oleszek, Izabela Krzeminska
Summary: The study found that ultrasonic pretreatment has significantly different effects on high-protein microalgal biomass compared to carbohydrate-rich microalgae, showing positive changes in methane fermentation process by decreasing structural complex carbohydrates content in one case and negative impacts on methane fermentation due to protein aggregation and formation of NH2 group in the other.
Article
Chemistry, Physical
Ibrahim Alfurayj, Rathiesh Pandian, Sarah Springer, Clemens Burda
Summary: A critical factor in achieving tunability in DESs is the variation of HBAs and HBDs. Choline fluoride-based DESs, such as EthalineF, were synthesized and characterized for the first time. Various spectroscopic techniques were used to study EthalineF, and measurements of polarity, density, viscosity, and conductivity were collected. The results showed that EthalineF has a larger solvodynamic radius compared to Ethaline, explaining the lower conductivity despite higher fluidity and solvation dynamics.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Applied
Shengnan Wang, Danni Qu, Guilan Zhao, Lina Yang, Lijie Zhu, Hong Song, He Liu
Summary: The microstructure and properties of the soy hull polysaccharide interfacial layer are significantly affected by pH, with higher stability at high pH levels but increased structural order at low pH levels. Low pH reduces thermal stability and increases viscosity of the emulsion, while high pH leads to higher viscosity.
Article
Chemistry, Physical
Shivaraj B. Radder, Raveendra Melavanki, Umesharaddy Radder, Sudhir M. Hiremath, Raviraj Kusanur, Seema S. Khemalapure
Summary: The present article presents the synthesis and characterizations of 3-(2-hydroxy-3-methoxy-phenyl) 1 (3-nitro-pheny1)-propenone using experimental techniques and computational methods. The comparison between experimental and theoretical data confirms the accuracy of the computational results and provides a better understanding of the experimental spectra. In addition, the properties of the molecule, including NMR analysis and molecular docking studies, are investigated.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Physical
A. Ram Kumar, S. Selvaraj, K. S. Jayaprakash, S. Gunasekaran, S. Kumaresan, J. Devanathan, K. A. Selvam, L. Ramadass, M. Mani, P. Rajkumar
Summary: The spectroscopic properties of syringaldehyde were studied using FT IR, FT Raman, and NMR techniques, as well as high-level quantum chemical calculations. The molecular properties of syringaldehyde were investigated to confirm its structure, and both experimental and theoretical findings were in complete agreement.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Chemistry, Physical
Sibel Bilgili, Fehmi Bardak, Etem Kose, Ahmet Atac
Summary: This study examines the spectroscopic and electronic properties of imidazole-based ionic liquids (ILs) using experimental and theoretical approaches. The study provides insights into the structural, vibrational, and electronic characteristics of ILs through various calculations and analytical techniques. The results contribute to a better understanding of the physicochemical properties of ILs.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Physical
Kalpana Sharma, Raveendra Melavanki, Sudhir M. Hiremath, Raviraj Kusanur, H. S. Geethanjali, Nagaraja Nagaraja
Summary: In this study, the compounds 3-(2,4-Dichloro-phenyl)-1-pyridin-3-yl-propenone (3DPP) and 5-Phenyl-1-pyridin-3yl-penta-2,4-dien-1-one (5PPD) were synthesized and characterized using spectroscopic and computational methods. The optimized structures and geometric parameters were obtained and compared with experimental data. Molecular docking studies and other analyses were performed to understand the properties of these compounds.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Physical
K. Vinoth, V Saravanakannan, P. Senthil Kumar, M. Maria Sylvester, D. J. S. Anand Karunakaran, Avadhut Ramram Deshmukh, T. Ganesh, A. C. Kumbharkhane
Summary: This study conducted a first characterization and signal analysis of low molecular weight aromatic amino acids, revealing their molecular dynamics, dielectric relaxation properties, as well as thermodynamic parameters and correlation factors. Confirmation of inter and intra molecular interactions and mixture groups was done through FT-IR, FT-Raman and NMR analysis.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Environmental Sciences
Awatief F. Hifney, Ayat Zien-Elabdeen, Mahmoud S. Adam, Mohamed Gomaa
Summary: The study successfully demonstrated the removal of ketoprofen and diclofenac from water using living cells of the green alga Chlorella sp., with adsorption efficiency being influenced by various parameters. The maximum adsorption capacities for KET and DIF were found to be 0.328 and 0.429 mg g(-1), respectively. The results suggest that microalgae living cells can serve as an eco-friendly and cost-effective biosorbent for the removal of pharmaceuticals at low concentrations.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Analytical
Guangyu Liu, Yujuan Hua, Ronda Gras, Jim Luong
Summary: An analytical strategy utilizing discrete frequency infrared imaging technique was developed to improve sample throughput in microplastic analysis. The concept demonstration study achieved high identification rates with a reduced scanning area, demonstrating the potential of this technique to enhance sample throughput.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Peter Capek, Zuzana Kostalova
Summary: The study found that wild blackthorn fruits are rich in polysaccharide complexes, including carbohydrates, phenolics, and proteins. Most of the material was released with 5% KOH and hot water. Based on the monosaccharide composition, the extracted fractions were divided into three groups, including highly esterified pectin material, a mixture of de-esterified pectin and hemicelluloses, and arabinogalactan and hemicelluloses. The blackthorn fruits exhibited significant antioxidant activity, especially the alkali-extracted fractions at concentrations of 0.02 and 0.05 mg/mL. Preliminary results suggest that blackthorn fruits may serve as an unexplored natural source of antioxidants.
CARBOHYDRATE RESEARCH
(2022)
Article
Chemistry, Applied
Edith Mawunya Kutorglo, Frantisek Muzika, Fatima Hassouna, Giuseppe Storti, Dusan Kopecky, Roman Bleha, Andriy Synytsya, Ivona Sedlarova, Miroslav Soos
Summary: Immobilizing enzymes on 3D porous materials can enhance stability and reusability, and the 3D porous materials synthesized using a template method with chemically accessible surfaces show potential as biocatalyst supports.
MICROPOROUS AND MESOPOROUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
YuJin Noh, Hwanhui Lee, Myeongsun Kim, Seong-Joo Hong, Hookeun Lee, Dong-Myung Kim, Byung-Kwan Cho, Choul-Gyun Lee, Hyung-Kyoon Choi
Summary: The study examined the effects of exogenous glucose on Synechocystis 7338 in terms of cell growth, pigments, metabolites, and lipids, showing that glucose supplementation can increase cell growth and production, making it a promising strategy for industrial applications.
Article
Food Science & Technology
Roman Bleha, Tetiana Shevtsova, Martina Zivcakova, Anna Korbarova, Martina Jezkova, Ivan Salon, Jan Brindza, Andriy Synytsya
Summary: Bee pollen samples were discriminated using vibrational spectroscopic methods by connecting with botanical sources, composition, and color. Fourier transform mid- and near-infrared (FT-MIR, FT-NIR), and FT-Raman spectra of bee pollen samples were measured and processed by principal component analysis (PCA), showing marked sensitivity to bee pollen composition. Furthermore, the combination of imaging, spectroscopic, and statistical methods is a potent tool for bee pollen discrimination.
Article
Polymer Science
Tamilla Mirzayeva, Jana Copikova, Frantisek Kvasnicka, Roman Bleha, Andriy Synytsya
Summary: This study utilized Fourier-transform infrared spectroscopy to screen the chemical composition of dietary supplements containing hyaluronic acid, and successfully quantified hyaluronic acid using various isolation procedures and analytical methods.
Article
Polymer Science
Roman Bleha, Lucie Tresnakova, Leonid Sushytskyi, Peter Capek, Jana Copikova, Pavel Kloucek, Ivan Jablonsky, Andriy Synytsya
Summary: This study focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum. Different extraction methods were used to obtain polysaccharide fractions, and their purity, composition, and structure were characterized using various analysis techniques. The main polysaccharides identified were branched O-2-beta-d-mannosyl-(1 -> 6)-alpha-d-galactan and highly branched (1 -> 3)(1 -> 4)(1 -> 6)-beta-d-glucan in the cold water and hot water extracts, respectively.
Article
Polymer Science
Yana Troshchynska, Roman Bleha, Andriy Synytsya, Jiri Stetina
Summary: When seeds become wet, viscous matter is secreted to support germination. Flaxseed mucilage (FSM) is such a material that is attractive for various applications. FSM is mainly composed of polysaccharides, proteins, minerals, and phenolic compounds. The genotype and harvest year of the flax seeds significantly influence the composition and properties of FSM. The rheological properties of FSM are determined by the ratio of polysaccharides and the contribution of proteins.
Article
Biochemistry & Molecular Biology
Leonid Sushytskyi, Andriy Synytsya, Tamilla Mirzayeva, Tereza Kalouskova, Roman Bleha, Jana Copikova, David KubaC, Tomas Grivalsky, Pavel Ulbrich, Petr Kastanek
Summary: The water-insoluble part of Parachlorella kessleri HY1 biomass was subjected to various extraction methods to obtain cell-wall polysaccharides. Proteins and different polysaccharides were identified and their potential role in the cell wall structure of microalgae was discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Seong Cheol Kim, Hyeon Jeong Kim, Gi Eun Park, Chang Won Lee, Andriy Synytsya, Peter Capek, Yong Il Park
Summary: In this study, the anti-osteoporotic activity of a sulfated glucuronorhamnoxylan polysaccharide from Capsosiphon fulvescens was evaluated using osteoclastic cells and ovariectomized mice. The polysaccharide significantly inhibited the differentiation and function of osteoclasts, as well as promoted bone mineral density and serum osteoprotegerin/RANKL ratio in the mice. These findings suggest that the polysaccharide has potential as a novel anti-osteoporotic compound.
MARINE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jan Stastny, Petr Marsik, Jan Tauchen, Matej Bozik, Anna Mascellani, Jaroslav Havlik, Premysl Landa, Ivan Jablonsky, Jakub Treml, Petra Herczogova, Roman Bleha, Andriy Synytsya, Pavel Kloucek
Summary: Edible medicinal mushrooms within the higher fungi group have a long history of being used for food and folk medicine. The Pleurotus genus, represented by Pleurotus ostreatus, has been widely cultivated and studied. In this study, lesser-known Pleurotus species were examined for their antioxidant and anti-inflammatory activities. Significant differences were found among the extracts, with P. flabellatus showing strong radical scavenging activity and P. ostreatus Florida demonstrating potent NF-kappa B inhibition. These findings suggest the therapeutic potential of these mushrooms for further investigation and clinical trials.
Review
Food Science & Technology
Leonid Sushytskyi, Andriy Synytsya, Jana Copikova, Pavol Lukac, Lenka Rajsiglova, Paolo Tenti, Luca E. Vannucci
Summary: For centuries, oats have been cultivated by human civilization and consumed in various forms of food. They are rich in beta-D-glucans, which have various biological effects including regulating blood cholesterol, anti-inflammatory properties, prebiotic effects, and antioxidant and tumor-preventing properties. Recent studies have highlighted the differences in biological properties based on the molecular weight of oat beta-D-glucan fractions. This review aims to provide a concise overview of the properties and studies of oat beta-D-glucan preparations based on their molecular weight and their potential in functional food to prevent or modulate various pathological conditions.
Article
Microbiology
Liudmila Kalitukha, Roman Bleha, Andriy Synytsya, Janina Kraska, Miriam Sari
Summary: This study focused on the composition, properties and potential use of a gel-forming extract from Auricularia heimuer. The extract contained soluble homo- and heteropolysaccharides, minerals, fatty acids, and amino acids. It exhibited thermal and storage stability, as well as a moisture retention capacity comparable to high molecular weight sodium hyaluronate. Hydrocolloids from Auricularia have great application potential in the food and cosmetic industries.
Article
Spectroscopy
Alla Synytsya, Daniela Janstova, Miroslava Smidova, Andriy Synytsya, Jaromir Petrtyl
Summary: Vibrational spectroscopic methods are used for molecular diagnostics of carcinogenesis. Collagen, as a component of connective tissue, serves as a biochemical marker for pathological changes in tissues. Differences in collagen vibrational bands can distinguish between normal colon tissue, benign, and malignant colon polyps. Specific collagen markers were found in the FTIR and Raman spectra of colon tissue samples and purified human collagens, which can be used for early diagnosis of colorectal carcinoma when combined with colonoscopy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Food Science & Technology
Evzen Sarka, Andrej Sinica, Petra Smrckova, Marcela Slukova
Summary: This review paper focuses on the recent advancements in the isolation, modification, and characterization of novel starches from botanical sources and food wastes, as well as their properties and applications. It highlights the unique properties of small starch granules and suggests their potential use in various industries. Starch plays a crucial role as a thickening agent in the food industry, and low-digestible starches could help reduce obesity and civilization diseases. The paper also discusses analytical methods and the use of non-traditional starches in industrial materials.
Article
Biotechnology & Applied Microbiology
Hwanhui Lee, YuJin Noh, Seong-Joo Hong, Hookeun Lee, Dong-Myung Kim, Byung-Kwan Cho, Choul-Gyun Lee, Hyung-Kyoon Choi
Summary: The study investigated the effects of different NaCl concentrations on Synechocystis 7338, a marine cyanobacterium with potential for producing photosynthetic pigments. Results showed that the highest production of chlorophyll a, allophycocyanin, and phycoerythrin occurred under 1.2 M NaCl conditions. Metabolic and lipidomic profiles were altered, and photosynthetic activity recovered under high salinity conditions. Increased hydrogen peroxide content and glutathione levels were observed, indicating adaptation of Synechocystis 7338 to high salt environments for enhanced production of bioactive metabolites.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
