期刊
BIORESOURCE TECHNOLOGY
卷 143, 期 -, 页码 18-24出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.05.102
关键词
Corncob acid hydrolysate; Microbial oil; Trichosporon catuneum; Medium composition; Cultural condition
资金
- Natural Science Foundation of Guangdong Province [S2012040007546]
- Support Plan Project of National Science and Technology [2012BAD32B07]
- National Natural Science Foundation of China [U1261116]
- Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences [y107rf1001]
The effect of medium composition and cultural condition on the growth and lipid accumulation of oleaginous yeast Trichosporon cutaneum on corncob acid hydrolysate was systematically investigated. Glucose, xylose, and cellobiose were shown to be promising sugar for lipid production by T. cutaneum. Adding other nitrogen sources into the hydrolysate was not beneficial for the lipid production possibly due to the existence of other nitrogen sources in it. Interestingly, adding MgSO4 center dot 7H(2)O, CuSO4 center dot 5H(2)O, MnSO4 center dot H2O, and KCl (optimal concentration were 0.3, 3.0 x 10(-3), 3.0 x 10(-3), and 0.4 g/L, respectively) could stimulate the lipid production by T. cutaneum. Additionally, inoculum concentration, temperature, and initial pH (optimal value were 5%, 28 degrees C, and 6.0, respectively) showed influence on the lipid production of T. cutaneum. Under the optimum conditions, the biomass (22.9 g/L) had a weak increase (3.6%), while the lipid content (45.4%) and lipid coefficient (22.9%) increased obviously (about 26.5% and 31.6%) compared with the initial conditions. (C) 2013 Elsevier Ltd. All rights reserved.
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