期刊
BIORESOURCE TECHNOLOGY
卷 115, 期 -, 页码 16-20出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.11.063
关键词
Hybrid biocatalyst; Anion exchanger; Dye decoloration; Stability; Reuse
资金
- NSFC [21177059/51078179]
- Fundamental Research Funds for the Central University of China
- New Century Excellent Talents in University of China [NCET-10]
A highly efficient and stable biocatalyst (denoted D201_Lac) was fabricated by encapsulating Trametes versicolor laccase within a macroporous and strongly basic exchange resin D201 through a simple adsorption process. Transmission electron micrographs and Fourier transform infrared spectra of the resultant D201_Lac proved that nanosized laccase clusters were embedded into the inner nano-pores/channels of 0201. As compared to the free laccase, D201_Lac showed enhanced resistance in the pH range of 3-7 or at temperature of 30-60 degrees C. Besides, negligible laccase was leached out from the host polymer D201 in solution of pH 3-7 and NaCl concentration up to 0.5 M, which might be attributed to the electrostatic attraction and the possible twining between long-chain laccase and the cross-linking host resin. Continuous seven-cycle batch decoloration of malachite green demonstrates that decoloration efficiency of D201_Lac kept constant for more than 320-h operation. (C) 2011 Elsevier Ltd. All rights reserved.
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