Article
Green & Sustainable Science & Technology
Yun Huang, Peirong Li, Yong Huang, Ao Xia, Xun Zhu, Qiang Liao
Summary: Microalgae biofilm is a porous structure that achieves simultaneous growth and lipid enrichment through photoautotrophic-heterotrophic symbiosis, solving the contradiction between microalgae growth and lipid accumulation, and providing a new approach for converting wastewater resources into clean biofuels.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agronomy
Wei Xing, Ruihao Zhang, Qun Shao, Chunxiao Meng, Xiaodong Wang, Zuoxi Wei, Fengjie Sun, Chang Wang, Kai Cao, Bingkui Zhu, Zhengquan Gao
Summary: This study investigated the effects of laser irradiation and heterotrophic cultivation on lipid productivity, content, and biomass of two Chlorella strains. The results showed that laser treatment can significantly increase biomass and lipid content in microalgae cells, indicating its potential for screening economically important oleaginous microalgae strains.
Article
Engineering, Environmental
Laure Wetterwald, Antoine Leybros, Gatien Fleury, Florian Delrue, Alexandra Dimitriades-Lemaire, Paul Chambonniere, Audrey Hertz
Summary: This study investigates the effects of water content, operating pressure and temperature on supercritical CO2 extraction of lipids from Chlorella vulgaris microalgae. The highest extraction yield was obtained with freeze-dried microalgae at 40℃ and 200 bar. However, extracts obtained at 40℃ and 250 bar had the highest fatty acid content, while those obtained at 60℃ and 250 bar had the highest cetane number and oxidative stability. Water in the initial biomass had a negative impact on total extraction yield, but promoted the extraction of fatty acids, particularly monounsaturated ones.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Hyun-Sik Yun, Young-Saeng Kim, Ho-Sung Yoon
Summary: The study found that the addition of glucose under mixotrophic conditions improved biomass yield and the content of useful substances in microalgae. Optimal cultivation results were obtained when 15-20 g L-1 initial glucose was provided under mixotrophic conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tasneema Ishika, Emeka G. Nwoba, Cherono Kwambai, Navid R. Moheimani
Summary: The harvesting frequency significantly influences the biomass productivity and lipid content of Nannochloropsis. High frequency harvesting is preferred for generating biomass, while lowering the frequency can enhance the lipid content. Proper management of harvesting frequency is crucial to achieve optimum targeted product (biomass and/or lipid).
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Liang Yu, Tingting Li, Jingwei Ma, Quanbao Zhao, Pierre Wensel, Jieni Lian, Shulin Chen
Summary: In this study, a growth model was developed and validated to predict the growth and lipid accumulation rates of Chlorella sorokiniana under heterotrophic and mixotrophic conditions. The model confirmed that better growth performance and higher lipid production are achieved under mixotrophic conditions compared to heterotrophic conditions.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemical Research Methods
Camila D. Coronel, Leonardo Curatti
Summary: This study provides a semi-empirical estimation of microalgal productivity in South America through simulation of cultivation conditions in different geographical locations. Results suggest that most productive regions for microalgal biomass and lipids are in the subtropical and tropical regions of the continent, with Fortaleza in Brazil showing productivity close to the target in 5-cm deep ponds.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Zhenyu Zhang, Li Wang, Yinqiang Wu, Chenshuo Li, Pengcheng Fu, Jing Liu
Summary: In this study, a novel subtropical microalgal strain, Chlorella vulgaris HNUFU001, with high selenium (Se) tolerance was isolated using a high Se concentration medium and a heterotrophic isolation strategy. The results showed that this strain exhibited a high Se removal rate and the ability to accumulate organic Se intracellularly, making it a promising candidate for the heterotrophic production of organic Se-related nutraceuticals.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Engineering, Environmental
Rocca C. Chin-On, Maria J. Barbosa, Rene H. Wijffels, Marcel Janssen
Summary: Microalgae are a promising renewable feedstock for various biobased products. To reduce production costs, a novel V-shaped photobioreactor is proposed to capture and dilute sunlight, thereby improving photosynthetic efficiencies and biomass productivities of microalgae.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Deyong Che, Liye Wang, Hongpeng Liu, Baizhong Sun, Shuai Guo
Summary: Biomass pyrolysis has been hindered by the formation of NOx precursors, mainly from protein pyrolysis. The effect of lipids on this process is unclear. In this study, proteins and lipids were extracted from chlorella and sewage sludge to investigate their thermal decomposition characteristics, thermodynamic properties, kinetic parameters, and reaction mechanisms. The results showed that lipids restrict the pyrolysis of sludge protein, while there was no significant difference in the pyrolysis of chlorella protein with or without lipids.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Green & Sustainable Science & Technology
Jun-Zhi Liu, Jin-Ye Yin, Hou-Feng Han, Ya-Ming Ge, Zheng-Yan Wang, Xing-Yan Bao, Feng Gao
Summary: This study improved the lipid productivity of Chlorella pyrenoidosa in municipal wastewater by adding iron and acetate supplements, resulting in increased lipid content and productivity. Acetate positively influenced the saturation degree of the algal fatty acid methyl esters and enhanced phosphorus utilization efficiency in algae cultivation.
Article
Biotechnology & Applied Microbiology
Yunlei Fu, Lanbo Yi, Shufang Yang, Xue Lu, Bin Liu, Feng Chen, Junchao Huang, Kawing Cheng, Han Sun, Xiaolei Wu
Summary: A novel cultivation method involving light induction was proposed in this study to enhance lutein production in Chlorella protothecoides CS-41 during heterotrophic cultivation. The results showed that low-light induction of seeds further increased biomass and lutein content. This cultivation strategy effectively enhances lutein yields, reduces production costs, and holds potential for broader application in other algal species for pigment production.
FERMENTATION-BASEL
(2023)
Article
Engineering, Environmental
Xue Li, Mingming Song, Ze Yu, Chen Wang, Jing Sun, Kunyang Su, Na Liu, Yiwen Mou, Tianxiang Lu
Summary: The study demonstrates the potential of microalga Chlorella pyrenoidosa in utilizing the low-cost carbon source acetic acid (AcA) for biomass and lipid accumulation, with the highest productivity achieved at 10 g/L of AcA in a heterotrophic mode. This promising method not only improves lipid production but also shows high nutrient removal efficiency and AcA assimilation efficiency, highlighting a win-win approach for AcA recycling and microalgae-based biodiesel production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Arash Karimian, Mahmood A. Mahdavi, Reza Gheshlaghi
Summary: The present study investigates the impact of trophic modes, organic carbon sources, and operation modes on the production of Chlorella sorokiniana IG-W-96 biomass and intracellular macromolecules. The results demonstrate significant variations among different cultivation strategies and provide guidelines and recommendations for the development of the biorefinery concept.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Energy & Fuels
Joana Oliveira, Emanuel Costa, Joana Maia Dias, Jose C. Pires
Summary: This study evaluates the feasibility of using microalgae as oil feedstock for biodiesel production. The results show that oil extracted at room temperature has a higher biodiesel conversion yield compared to high-temperature extraction. The potential of using Chlorella vulgaris for biodiesel production is low, but enzymatic biodiesel production from microalgae represents a promising avenue for sustainable energy generation.
BIOENERGY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Fanxue Zeng, Jianke Huang, Chen Meng, Fachao Zhu, Jianpei Chen, Yuanguang Li
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2016)
Article
Agricultural Engineering
Chun-Dan Zhang, Wei Li, Yun-Hai Shi, Yuan-Guang Li, Jian-Ke Huang, Hong-Xia Li
BIORESOURCE TECHNOLOGY
(2016)
Article
Agricultural Engineering
Jianke Huang, Jiangguo Ying, Fei Fan, Qijian Yang, Jun Wang, Yuanguang Li
BIORESOURCE TECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Jianke Huang, Qijian Yang, Jianpei Chen, Minxi Wan, Jiangguo Ying, Fei Fan, Jun Wang, Wei Li, Yuanguang Li
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2016)
Article
Biotechnology & Applied Microbiology
Dongda Zhang, Minxi Wan, Ehecatl A. del Rio-Chanona, Jianke Huang, Weiliang Wang, Yuanguang Li, Vassilios S. Vassiliadis
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2016)
Article
Biotechnology & Applied Microbiology
Bin Sheng, Fei Fan, Jianke Huang, Wenmin Bai, Jun Wang, Sulan Li, Wei Li, Minxi Wan, Yuanguang Li
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2018)
Article
Biotechnology & Applied Microbiology
Wenchao Cheng, Jianke Huang, Jianpei Chen
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Zhen Zhang, Yingying Tan, Weiliang Wang, Wenmin Bai, Jianhua Fan, Jianke Huang, Minxi Wan, Yuanguang Li
JOURNAL OF APPLIED PHYCOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Jianke Huang, Ben Hankamer, Jennifer Yarnold
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2019)
Article
Biotechnology & Applied Microbiology
Haohua Wang, Zhen Zhang, Minxi Wan, Ruixuan Wang, Jianke Huang, Kaikai Zhang, Jiacai Guo, Wenmin Bai, Yuanguang Li
JOURNAL OF APPLIED PHYCOLOGY
(2020)
Article
Agricultural Engineering
Minxi Wan, Zhen Zhang, Ruixuan Wang, Wenmin Bai, Jianke Huang, Weiliang Wang, Guomin Shen, Anquan Yu, Yuanguang Li
BIOMASS & BIOENERGY
(2019)
Article
Microbiology
Dao-Feng Zhang, Xi-Wen Cui, Zhe Zhao, Ai-Hua Zhang, Jian-Ke Huang, Wen-Jun Li
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Jinshun Jiang, Jianke Huang, Han Zhang, Zhen Zhang, Yi Du, Zheng Cheng, Bo Feng, Ting Yao, Aihua Zhang, Zhe Zhao
Summary: The use of the diatom Phaeodactylum tricornutum in treating swine wastewater and producing fucoxanthin shows great potential. The alga has the highest nutrient removal efficiency when the ratio of swine wastewater to water is 1:1. This study demonstrates the environmentally friendly approach of P. tricornutum in swine wastewater treatment and fucoxanthin production.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ai Hua Zhang, Bo Feng, Han Zhang, Jinshun Jiang, Daofeng Zhang, Yi Du, Zheng Cheng, Jianke Huang
Summary: In this study, different percentages of diluted swine wastewater were used to cultivate the red alga Porphyridium purpureum, and its microalgal growth, nutrient removal, polysaccharide production, and phycoerythrin production were evaluated. The results showed that 60% diluted swine wastewater resulted in the best growth and nutrient removal, and high concentrations of polysaccharide and phycoerythrin were produced. This indicates that P. purpureum has great potential in treating wastewater and producing high-value byproducts.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jianke Huang, Chao Zhang, Han Zhang, Ting Yao, Yi Du, Zheng Cheng, Ai-Hua Zhang, Daofeng Zhang, Zhen Zhang
Summary: Adding organic compounds to wastewater can promote microalgae growth and remove nutrients. In this study, Chlorella pyrenoidosa was cultured in a mixture of swine wastewater and furfural wastewater. The results showed that the mix ratio of the two wastewaters had a significant impact on microalgae growth, nutrient removal efficiency, and lipid accumulation. Proper mix ratios could enhance the removal of nutrients and increase lipid productivity.
APPLIED SCIENCES-BASEL
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
