期刊
BIORESOURCE TECHNOLOGY
卷 120, 期 -, 页码 264-272出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.06.002
关键词
Cellulase; Populus biomass; Response surface methodology optimization; Saccharification; Pholiota adiposa
资金
- Konkuk University
A basidiomycetous fungus, identified as Pholiota adiposa SKU0714 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase and beta-glucosidase activities of 26, 32 and 39 U/mL, respectively and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1680, 0.12, 65 and 0.41 U/mL, respectively when grown with rice straw as a carbon source. Among the various plant biomasses tested for saccharification, poplar biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize hydrolysis parameters. A maximum saccharification yield of 83.4% (667 mg/g-substrate), the highest yield from any plant biomass, was obtained with Populus biomass after 24 h of hydrolysis. P. adiposa was proven to be a good choice for the production of reducing sugars from cellulosic biomass. (C) 2012 Elsevier Ltd. All rights reserved.
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