期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 11, 页码 6600-6604出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.03.070
关键词
Succinate; PHA; Escherichia coli; Metabolic engineering; Biorefinery
资金
- Shandong Science and Technology Development Plan [2010GSF10202]
- National Natural Science Foundation of China [30870022]
- National Basic Research Program of China [2011CB707405]
The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07 g/L succinate and 0.54 g/L PHA (5.62 wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7 mol% 3-hydroxyoctanoate (3HO) and 41.3 mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据