Article
Engineering, Environmental
Zhaopeng Qu, Jin Li, Zhi Hu, Wenzong Liu, Aijie Wang
Summary: Currently, conventional biological nitrogen removal processes face challenges in removing ammonium from saline wastewater. This study successfully utilized marine anammox bacteria in a microbial electrolytic cell to convert NH4+-N to N2. In addition, the application of voltage increased microbial diversities at the electrode surface and promoted the formation of electrode biofilm.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Bin Ma, Yanbing Liang, Yujian Zhang, Yan Wei
Summary: A partial-nitrification/anammox and endogenous partial-denitrification/anammox (PN/A-EPD/A) process was developed in a sequential batch biofilm reactor (SBBR) to achieve advanced nitrogen removal from low-carbon wastewater. The effluent total nitrogen (TN) reached 3.29 mg/L when the influent COD/TN and the TN were 2.86 and 59.59 mg/L, respectively. The stable PN/A-EPD/A process was achieved through four strategies, including treating the inoculated sludge with free nitrous acid, inoculating anammox biofilm, discharging excess activated sludge and residual ammonium at the end of oxic stage. The 16S rRNA high-throughput sequencing results showed the coexistence of anammox bacteria, ammonia oxidizing bacteria, nitrite oxidizing bacteria, denitrifying glycogen accumulating organisms (DGAOs) and denitrifying phosphorus accumulating organisms (DPAOs) in the biofilms, with higher abundance of anammox bacteria in the inner layer and higher abundance of DGAOs and DPAOs in the outer layer.
BIORESOURCE TECHNOLOGY
(2023)
Review
Environmental Sciences
Hussein E. Al-Hazmi, Gamal K. Hassan, Mojtaba Maktabifard, Dominika Grubba, Joanna Majtacz, Jacek M. Akinia
Summary: This review comprehensively presents and discusses the latest advances in nitrogen removal technologies from wastewaters, including traditional nitrification-denitrification and anammox-based systems. It focuses on the understanding of microbial community characteristics and optimization of operation parameters in anammox-based systems. The sustainability of BNR processes in wastewater treatment is evaluated using important indicators such as GWP, CF, and energy behaviors. The challenges and solutions for BNR processes are extensively discussed.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Chunhua He, Xuesong Ren, Guoqing Xu, Zhiqiang Huang, Yulan Wang, Zhenhu Hu, Wei Wang
Summary: Phenol and ammonia are common pollutants in industrial wastewaters. The study successfully started and operated a single-stage partial nitritation /anammox reactor treating phenol-ammonia wastewater, achieving total nitrogen and COD removal efficiencies of around 73.0% and 79.5%. The anammox bacteria adapted to phenol condition and remained stable activity after 60 days of operation.
WATER ENVIRONMENT RESEARCH
(2021)
Article
Engineering, Environmental
Hussein E. Al-Hazmi, Dominika Grubba, Joanna Majtacz, Aleksandra Ziembinska-Buczynska, Jun Zhai, Jacek Makinia
Summary: Recently, the combined partial denitrification and anammox (PD/anammox) process using organic matter in municipal wastewater has gained attention for sustainable N removal. It offers advantages such as reduced dissolved oxygen demand, lower organic carbon requirement, less excess sludge production, and lower greenhouse gas emissions compared to conventional nitrification-denitrification. This review discusses the latest improvements in PD/anammox systems and focuses on the production/consumption pathways of critical intermediate NO2-. The C/N ratio and concentration of dosed organic carbon are crucial for optimal efficiency, as overgrowth of denitrifying bacteria can negatively impact anammox activity. Microorganism competition in single-stage PD/anammox systems plays a key role in achieving efficient N removal from mainstream wastewater. Challenges and limitations in recognizing inhibition mechanisms and performance inhibitors are also addressed. Future research should explore combining PD/anammox with other processes and developing self-supporting bacterial systems to enhance total nitrogen removal efficiency.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Agricultural Engineering
Haike Xiao, Yongzhen Peng, Qiong Zhang, Ying Liu
Summary: This study demonstrated the feasibility of starting and maintaining partial nitrification through pre-anaerobic treatment, as well as the feasibility of nitrogen removal by coupling simultaneous nitrification-denitrification with anammox to treat actual municipal sewage.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Xiangchen Li, Yongzhen Peng, Jingwen Zhang, Rui Du
Summary: Anaerobic ammonium oxidation (anammox) is a widely accepted energy-efficient method for nitrogen removal from high-strength sidestreams. This study demonstrated highly efficient and synchronous nitrogen removal by coupling anammox with a double-nitrite-shunt process, achieving TN removal efficiency of up to 98.4% even at low temperatures. The integration of partial denitrification with anammox offered an efficient solution to address the challenge of excessive NO3--N residue, showcasing significant savings in energy and organic carbon compared to conventional methods.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Environmental
Yuanyuan Miao, Liang Zhang, Deshuang Yu, Jianhua Zhang, Wenke Zhang, Guocheng Ma, Xinchao Zhao, Yongzhen Peng
Summary: Intermittent aeration is an effective strategy for biological wastewater treatment, allowing for advanced nitrogen removal and reduced energy consumption. It is suitable for partial nitrification and denitrification processes.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Lisheng Wang, Wancong Gu, Yanchen Liu, Peng Liang, Xiaoyuan Zhang, Xia Huang
Summary: This paper summarizes the challenges and solutions of mainstream anammox-based process by reviewing the literature of the past decade. The slow growth rate of anammox bacteria and the need for enhancing bacteria retention are identified as the main challenges. Various methods to culture anammox bacteria and improve their activity are discussed. Other challenges include the elimination of nitrite oxidizing bacteria (NOB) and achieving the ideal ratio of NH4+ and NO2-. To overcome these challenges, composite control strategies based on low sludge retention time (SRT) and limited aeration are suggested. Interference from low temperature and influent components in actual wastewater treatment is addressed, as well as the use of coupling processes to reduce nitrate concentration in the effluent. The paper concludes with future prospects for the mainstream anammox-based process.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Yuancan Zhang, Yajie Qiao, Zhimin Fu
Summary: This study investigated the feasibility of simultaneous denitrification phosphorus removal (DPR) partial nitrification (PN) and anammox (AMX) process in a coupling three-stage sequencing batch reactors (SBR) system for treating municipal sewage. The results showed high removal efficiencies for total nitrogen (TN), phosphorus, and chemical oxygen demand (COD). The periodic experiment results revealed the contributions of anoxic and aerobic phosphorus uptake. The microbial community and functional groups were also analyzed during the coupling operation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Hong Chen, Zhi Tu, Sha Wu, Guanlong Yu, Chunyan Du, Hong Wang, Enzhe Yang, Lu Zhou, Bin Deng, Dongbo Wang, Hailong Li
Summary: The novel process of partial denitrification (PD) coupled with anaerobic ammonium oxidation (AMX) shows great potential for nitrogen removal in municipal wastewater treatment. Research focuses on process mechanisms, microbial community characteristics, key operational parameters, stable operation, and control methods. Challenges lie in engineering and economic feasibility, full mainstream AMX application, and further study of PD-AMX mechanism optimization for better engineering practice.
Article
Environmental Sciences
Jianzheng Li, Kaiwen Deng, Jia Meng, Jiuling Li, Min Zheng
Summary: A biofilm-based anaerobic-aerobic (A(2)O(2)) reactor was constructed for treating manure-free piggery wastewater, achieving high-level nutrient removal with NH4+-N, TN, and COD removal efficiencies as high as 99.4%, 91.7%, and 79.4%, respectively. The results showed that COD and nitrogen removal occurred mainly in the anaerobic and aerobic phases, respectively.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Review
Engineering, Environmental
Quan Zhang, Xijun Xu, Ruochen Zhang, Bo Shao, Kaili Fan, Lei Zhao, Xiaoming Ji, Nanqi Ren, Duu-Jong Lee, Chuan Chen
Summary: This review summarizes the recent advances and characteristics of the mixed/mixotrophic nitrogen removal (MixNR) process guided by sulfur-driven autotrophic denitrification (SDAD) and anammox. It discusses the functional microorganisms in different MixNR systems, sheds light on metabolic mechanisms and microbial interactions, and highlights the importance of MixNR for carbon reduction in the BNR process. Knowledge gaps and future research directions that may facilitate the practical application of the MixNR process are also emphasized.
Article
Engineering, Environmental
Xinjie Gao, Qiong Zhang, Zeming An, Baishou Shao, Yongzhen Peng
Summary: Anaerobic ammonium oxidation (anammox) combined with partial nitrification (PN) can achieve energy self-sufficiency in wastewater treatment. The PNA process is beneficial for advanced and efficient nitrogen removal in carbon-limited wastewater treatment.
RESOURCES CONSERVATION AND RECYCLING
(2023)
Article
Engineering, Environmental
Xinyi Zou, Mengjie Ma, Jiachen Zhu, Qun Zhang, Qianfei Cao, Sami Khasraw Hamid, Chongjun Chen
Summary: The study found that salinity, represented by SO42-, has an impact on the anammox reaction. Increasing concentrations of SO42- decreased the removal efficiency of NH4+-N and NO2--N, as well as affected the sludge characteristics and microbial community structure.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Environmental Sciences
Hisashi Satoh, Kai Kikuchi, Yutaka Katayose, Shu Tsuda, Reiko Hirano, Yuga Hirakata, Masaaki Kitajima, Satoshi Ishii, Mamoru Oshiki, Masashi Hatamoto, Masahiro Takahashi, Satoshi Okabe
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Microbiology
Takahiro Segawa, Nozomu Takeuchi, Hiroshi Mori, Rathnayake M. L. D. Rathnayake, Zhongqin Li, Ayumi Akiyoshi, Hisashi Satoh, Satoshi Ishii
FEMS MICROBIOLOGY ECOLOGY
(2020)
Article
Chemistry, Physical
Yu Otsuka, Guanglei Li, Hiromi Takahashi, Hisashi Satoh, Koji Yamada
Article
Environmental Sciences
Meri Nakajima, Reiko Hirano, Satoshi Okabe, Hisashi Satoh
Summary: A simple assay for colorimetric quantification of bacterial 16S rRNA was developed, based on RNA extraction with commercial kits and mixing with Au-nanoprobes and NaCl. The assay format was validated and optimized, achieving linear calibration curve above 5.0 x 10(7) copies/mu L and a limit of detection of 1.17 x 10(8) copies/mu L. The quantification of bacterial 16S rRNA in activated sludge samples showed deviations between 48% and 208% compared to RT-qPCR results, introducing an innovative tool for stable treatment performance in wastewater processes.
Article
Mechanics
Yasunori Watanabe, Haruhi Oyaizu, Hisashi Satoh, Yasuo Niida
Summary: This study proposes novel probabilistic empirical models for the rise velocity and bubble drag of a microbubble swarm, incorporating bubble-vortex interactions. Convective interactions between bubbles and vortex-induced flows are found to accelerate bubbles and align them along paths in the flow, resulting in an increase in rise velocity and its variance in the statistical equilibrium state.
INTERNATIONAL JOURNAL OF MULTIPHASE FLOW
(2021)
Article
Engineering, Environmental
Hisashi Satoh, Yutaka Katayose, Reiko Hirano
Summary: A novel and simple method was developed to monitor Escherichia coli concentrations in river water, which was found to be reliable and applicable for estimating E. coli concentrations through monitoring GUS activities. The method is simple, rapid, reliable, inexpensive, and high throughput, making it useful for monitoring E. coli in river water.
WATER SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Environmental
Koji Matsunaga, Hisashi Satoh, Reiko Hirano
Summary: In this study, a simple analytical method using CeO(2)NPs and FAM-labeled DNA was developed to determine As(V) concentrations in groundwater. After optimizing the parameters, the total analysis time was about 20 min and the limit of detection was 0.61 μM. The method showed high selectivity against other metal ions.
Article
Engineering, Environmental
Koji Matsunaga, Yuki Nakaya, Hisashi Satoh
Summary: We investigated the interaction between As(III) and As(III)-binding DNA aptamers (Ars-3). The study found that Ars-3 cannot bind As(III), but As(III) can selectively adsorb onto gold nanoparticles. We developed simple analytical methods to measure the concentrations of As(III) and As(V) separately in ultrapure water and groundwater.
Article
Engineering, Environmental
Kyohei Kuroda, Takashi Narihiro, Yuki Nakaya, Taro Q. P. Noguchi, Ryota Maeda, Masaru K. Nobu, Yuki Ohnishi, Yasuhiro Kumaki, Tomoyasu Aizawa, Hisashi Satoh
Summary: With the increasing usage of plastics, the environmental risk associated with the disposal of waste plastics and discharge of microplastics has become a concern. This study established anaerobic microbial consortia that can degrade PET-derived materials and identified the degradation pathways and microorganisms involved.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Yuki Nakaya, Jinming Jia, Hisashi Satoh
Summary: In this study, a digital microscope and an automatic stage were used to rapidly obtain magnified floc images and calculate shape parameters. The morphological parameters and extracellular polymeric substances (EPS) quantity/quality of AS were analyzed in four wastewater treatment plants. The results showed that larger, denser, and smoother flocs tend to have better dewaterability and settlement properties.
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Engineering, Environmental
Mohomed N. M. Shayan, Yuna Tanaka, Reiko Hirano, Yuki Nakaya, Hisashi Satoh
Summary: Fecal pollution in urban rivers poses risks to human health and the environment. A simple and rapid method was developed in this study to detect fecal pollution in urban rivers. By measuring the beta-D-glucuronidase (GUS) activity of Escherichia coli (E. coli) cultures, fecal pollution sources could be distinguished from naturally occurring E. coli. This method can be used to detect fecal pollution in urban rivers by setting a threshold for GUS activity.
Article
Environmental Sciences
Meri Nakajima, Reiko Hirano, Yuki Nakaya, Hisashi Satoh
Summary: This article introduces a simple method for quantifying the concentration of functional bacteria's 16S rRNA in wastewater without reverse transcription of RNA or quantitative polymerase chain reaction (qPCR). The method was optimized and a calibration curve for ammonia-oxidizing bacteria (AOB) 16S rRNA was created, allowing for the quantification of AOB in wastewater treatment processes.
Article
Environmental Sciences
Hisashi Satoh, Natsumi Nagahashi, Kai Kikuchi, Reiko Hirano
Summary: Wastewater treatment plants have been identified as potential reservoirs of antibiotic-resistant bacteria. In this study, a novel simple testing method was developed to rapidly determine the concentration of antibiotic-resistant E. coli in wastewater and river water samples.
Article
Engineering, Environmental
Hisashi Satoh, Yukari Kashimoto, Naoki Takahashi, Takashi Tsujimura
Summary: This study developed two deep learning-based classifiers to recognize different morphologies of AS flocs and the abundance of filamentous bacteria within them. Through retraining, classifier-1 accurately distinguished between aggregated and dispersed flocs, and recognized morphological changes. Classifier-2 quantitatively identified the abundance of filamentous bacteria within the AS flocs.
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mamoru Oshiki, Haruna Hiraizumi, Hisashi Satoh, Satoshi Okabe
MICROBES AND ENVIRONMENTS
(2020)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
