期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 2, 页码 469-474出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.08.048
关键词
Consecutive microwave irradiation; Covalent immobilization; Mesocellular siliceous foams; Aldolase; 2-Deoxy-D-ribose-5-phosphate
资金
- Technology Research and Development Program of Hangzhou [20090331N03]
- National Natural Science Foundation of China [20906016]
- Science and Technology of Zhejiang Province [2009C33005]
- Special Foundation for New Researchers in Hangzhou Normal University [YS05203141]
- Natural Science Foundation of Zhejiang Province [Y4080317]
To establish a stable and efficient immobilization technique under microwave irradiation, a focused microwave reaction system was used, where the temperature was set appropriately in the microwave system and cooling module to produce consecutive microwave irradiation. 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) was rapidly and efficiently immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation. When the output power in the microwave system was set to 30W, after 3 min, 88.4% of the enzyme protein was coupled to the wall of the support pores and the specific activity of the immobilized enzyme was 2.24 U mg(-1), 149.2% higher than that of the free enzyme and 157.0% higher than that of the non-microwave-assisted immobilized enzyme. In catalysis, microwave-assisted immobilized DERA tolerated a wider range of both pH and temperature than other DERA preparations. The thermal and storage stabilities were also significantly improved. This focused: microwave-assisted immobilization technique has proven to be simple, stable and highly efficient. This technique could also be applied to other enzyme immobilizations. (C) 2010 Elsevier Lid. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据