期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 19, 页码 7587-7591出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.04.077
关键词
Cyanobacteria; Kjeldahl method; Lowry method; Microalgae; Protein content
资金
- Ministerio de Educacion y Ciencia [CTQ2004-07628-C02-01/PPQ]
- ENDESA
- Ministerio de Industria (CDTI)
- Junta de Andalucia and Plan Andaluz de Investigacion, Spain [BIO 173]
The protein content of dry biomass of the microalgae Porphyridium cruentum, Scenedesmus almeriensis, and Muriellopsis sp. and of the cyanobacteria Synechocystis aquatilis and Arthrospira platensis was measured by the Lowry method following disruption of the cells by milling with inert ceramic particles. The measurements were compared with the Kjeldahl method and by elemental analysis. The nitrogen-to-protein conversion factors for biomass obtained from exponentially growing cells with a steady state doubling time of similar to 23 h were 5.95 for nitrogen measured by Kjeldahl and 4.44 for total nitrogen measured by elemental analysis. The protein content in dry biomass ranged from 30% to 55%. The above conversion factors are useful for estimating the protein content of microalgal biomass produced in rapid steady state growth as encountered in many commercial production processes. (C) 2010 Elsevier Ltd. All rights reserved.
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