Article
Chemistry, Physical
Penghui Li, Xin Wang, Chunchun Zhang, Dingguo Xu
Summary: This study investigates the processive binding mechanism of cellulase family 9 (Cel9G) to polysaccharides. The simulations reveal that the binding of the cellulose chain exhibits several local minima, which are influenced by the CH/pi interactions between sugar rings and aromatic residues. Y520 is found to be critical for processive binding, and the straight-line morphology of the substrate is observed after the formation of the slide mode. The study proposes a four-step catalytic cycle for Cel9G.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Laure Ayme, Agnes Hebert, Bernard Henrissat, Vincent Lombard, Nathalie Franche, Stephanie Perret, Etienne Jourdier, Senta Heiss-Blanquet
Summary: The study identified six GH9 enzymes from a compost metagenome and characterized three of them, revealing their diverse structures and functional characteristics. These enzymes play a crucial role in bacterial biomass deconstruction.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Energy & Fuels
Youssef Bacila Sade, Camila Silva Goncalves, Sandra Mara Naressi Scapin, Guilherme Luiz Pinheiro, Roberto Becht Flatschart, Wanderley de Souza, Norton Heise, Ednildo de Alcantara Machado
Summary: This study reports the identification and characterization of a novel endoglucanase AfEG66 from the snail Achatina fulica, which shows high activity and stability against polysaccharide substrates, contributing to the improvement of production efficiency of green fuels.
BIOENERGY RESEARCH
(2022)
Article
Chemistry, Physical
Paripok Phitsuwan, Sengthong Lee, Techly San, Khanok Ratanakhanokchai
Summary: GH9 family endoglucanases play vital roles in cellulose degradation with diverse activity towards cellulose. We cloned and expressed a GH9 enzyme (CalkGH9T) from Clostridium alkalicellulosi, showing efficient hydrolysis of CMC but poor degradation of RAC.
Article
Biochemistry & Molecular Biology
Ardhendu Mandal, Abhijeet Thakur, Arun Goyal
Summary: This study investigated the biochemical properties of a recombinant 8-1,4 endoglucanase, AtGH9C-CBM3A-CBM3B, and the role of its associated CBMs in catalysis. The enzyme showed maximum activity at 55°C and pH 7.5, and exhibited high activity against carboxy methyl cellulose, lichenan, 8-glucan, and hydroxy ethyl cellulose. The catalytic module, AtGH9C, showed insignificant activity, emphasizing the essential requirement of CBMs in catalysis. The associated CBMs also contributed to thermostability. The results highlight the importance of the physical association and cross-talk between AtGH9C and its associated CBMs in efficient cellulose catalysis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Jorick Franceus, Jolien Lormans, Lore Cools, Matthias D'hooghe, Tom Desmet
Summary: The study demonstrates significant phosphorylase activity can be established in GH3 glycoside hydrolases through single-site substitutions and further optimized through mutational paths. These findings suggest new insights into developing phosphorylases and provide a novel approach for future research in this field.
Article
Biochemistry & Molecular Biology
Kedar Sharma, Carlos M. G. A. Fontes, Shabir Najmudin, Arun Goyal
Summary: The alpha-L-arabinofuranosidase from Clostridium thermocellum displays a modular architecture, enhancing catalytic efficiency. Molecular dynamics simulation analysis reveals higher flexibility in its C-terminal module. Small angle X-ray scattering analysis indicates an elongated structure of the enzyme.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Ninian Prem Prashanth Pabbathi, Aditya Velidandi, Pradeep Kumar Gandam, Prakash Koringa, Sreenivasa Rao Parcha, Rama Raju Baadhe
Summary: Lignocellulosic biomass is a prominent option for second-generation biofuels production. A novel cellulase, Cel-3.1, was identified from buffalo rumen and showed potential in hydrolyzing rice straw and corncob to generate fermentable sugars.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biotechnology & Applied Microbiology
Lirui Liu, Wen-Cong Huang, Yang Liu, Meng Li
Summary: Lignocellulosic biomass is a geographically abundant source of renewable energy with potential to meet increasing energy demands. Improving the efficiency and yield of cellulase production remains a significant research focus. Cellulases from bacteria and archaea are increasingly considered promising candidates for industrial applications.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Microbiology
Jeremy R. Ellis, James J. Bull, Paul A. Rowley
Summary: Commercially available cellulases and amylases can disperse bacteria embedded in biofilms, suggesting the potential use of enzyme therapy to treat biofilm-associated bacterial infections. However, only one of the 76 recombinant glycoside hydrolases tested showed activity in dispersing Staphylococcus biofilms. Commercial cellulase preparations contained multiple enzymes with biofilm-dispersing activity, indicating the need for large libraries and purified enzymes to prospect for additional antibiofilm enzymes.
Article
Chemistry, Physical
Krisztina Zajki-Zechmeister, Manuel Eibinger, Bernd Nidetzky
Summary: This study reveals the formation of transient clusters of endo-and exocellulases on the cellulose surface, leading to directed ablation of single microfibrils through a multilayer processive mode. The cooperativity between endo-and exocellulases enables faster degradation of structurally organized polymer materials.
Article
Biotechnology & Applied Microbiology
Aditi Konar, Shritama Aich, Ranaprathap Katakojwala, Supratim Datta, S. Venkata Mohan
Summary: This study focuses on GH9 endoglucanases and identifies an highly active enzyme, H1AD14, from Bacillus licheniformis. The enzyme shows high enzymatic activity on various substrates and exhibits salt tolerance and processivity. The findings contribute to the understanding of GH9 endoglucanases and offer potential for developing improved cellulase cocktails for biomass deconstruction.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Anke Chen, Dan Wang, Rui Ji, Jixi Li, Shaohua Gu, Rong Tang, Chaoneng Ji
Summary: In this study, a thermostable beta-glucosidase (TsBGL) was isolated and characterized from the Thermoprotei Thermofilum sp. ex4484_79, exhibiting high activity and specific activity at elevated temperatures and pH levels. The crystal structure of TsBGL reveals a classical (alpha/beta)(8)-barrel catalytic domain, with catalytic sites including Glu210 and Glu414. TsBGL shows promise for potential industrial applications with its high thermostability and catalytic activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Amisha Patel, Amita Shah
Summary: A novel thermostable GH5 family endoglucanase from a newly isolated brown rot fungi Fomitopsis meliae CFA 2 was purified, characterized and partially sequenced in this study. The enzyme exhibited high specificity, optimal temperature and pH conditions, and was stimulated by metal ions and DTT. Overall, the endoglucanase showed potential for applications in biomass hydrolysis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemical Research Methods
Krishan Kumar, Shubha Singh, Kedar Sharma, Arun Goyal
Summary: The study modeled the processive endocellulase HtGH9 from Hungateiclostridium thermocellum and found that it has a specific structure and catalytic mechanism.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2021)
Article
Construction & Building Technology
Guo Aofei, Sun Zhihui, Qi Chengqing, Noppadon Sathitsuksanoh
JOURNAL OF MATERIALS IN CIVIL ENGINEERING
(2020)
Article
Nanoscience & Nanotechnology
Emtias Chowdhury, Mohammad Shahinur Rahaman, Noppadon Sathitsuksanoh, Craig A. Grapperhaus, Martin G. O'Toole
Summary: The utilization of nanoparticle-polymer bead hybrid nanostructures as a SERS substrate requires precise control over the deposition, density, and distribution of the nanoparticles on the bead surface. Through a two-step DNA-mediated assembly, a large area SERS substrate was successfully fabricated, showing high density hot spots for enhanced Raman scattering. High detection sensitivity and enhancement factors were achieved for the analysis of probe molecules like Methylene blue.
Review
Chemistry, Multidisciplinary
Aofei Guo, Zhihui Sun, Noppadon Sathitsuksanoh, Hu Feng
Article
Chemistry, Physical
Md Anwar Hossain, Kyle N. Mills, Ashten M. Molley, Mohammad Shahinur Rahaman, Sarttrawut Tulaphol, Shashi B. Lalvani, Jie Dong, Mahendra K. Sunkara, Noppadon Sathitsuksanoh
Summary: In this study, catalysts with increased Lewis acid site density were prepared by heat treatment of ZSM-5, showing enhanced selectivity towards lactic acid. The Lewis acid sites were found to play a key role in converting pyruvaldehyde to lactic acid during dihydroxyacetone dehydration.
APPLIED CATALYSIS A-GENERAL
(2021)
Article
Agricultural Engineering
Md Anwar Hossain, Mohammad Shahinur Rahaman, Daniel Yelle, Hong Shang, Zhihui Sun, Scott Renneckar, Jie Dong, Sarttrawut Tulaphol, Noppadon Sathitsuksanoh
Summary: Deep eutectic solvent treatment shows great potential in overcoming lignocellulose resistance for biofuels and bioproducts production. Research focused on four solvents, with Choline chloride-glycerol pretreatment showing good results in retaining glucan from rice straw and fractionating lignin. However, some challenges remain with solvent deposition on solids limiting cellulose accessibility to enzymes.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Multidisciplinary Sciences
Mohor Chatterjee, Yu Fan, Fang Cao, Aaron A. Jones, Giovanni Pilloni, Xiaozhou Zhang
Summary: MIC is a major threat to carbon steel asset integrity in the oil and gas industry, requiring reliable monitoring methods. Proteins associated with MIC can serve as biomarkers for diagnosis and easy-to-implement immunoassays. Unique proteins linked to severe MIC were identified, including a multi-heme cytochrome protein possibly involved in electron transfer.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Mohammad Shahinur Rahaman, Sarttrawut Tulaphol, Md Anwar Hossain, Clint N. Evrard, Lee M. Thompson, Noppadon Sathitsuksanoh
Summary: Diphenolic acid (DPA) can be produced by condensation of phenol and levulinic acid, forming two isomers. Kinetic analysis reveals that the reaction rate is influenced by temperature and molar ratio.
MOLECULAR CATALYSIS
(2021)
Article
Chemistry, Physical
Mohammad Shahinur Rahaman, Sarttrawut Tulaphol, Kyle Mills, Ashten Molley, Md Anwar Hossain, Shashi Lalvani, Thana Maihom, Mark Crocker, Noppadon Sathitsuksanoh
Summary: Lactic acid is a renewable and versatile chemical derived from biomass, and the development of water-tolerant acid catalysts is crucial for its production. The study demonstrates that the MIL-101(Al)-NH2 metal-organic framework is an efficient and selective solid Lewis acid catalyst for dihydroxyacetone isomerization to lactic acid, showing high selectivity and stability.
Article
Energy & Fuels
Mohammad Shahinur Rahaman, Sarttrawut Tulaphol, Md Anwar Hossain, Jacek B. Jasinski, Ning Sun, Anthe George, Blake A. Simmons, Thana Maihom, Mark Crocker, Noppadon Sathitsuksanoh
Summary: The study developed a dual acid phosphotungstic acid-encapsulated MOF catalyst for efficient conversion of biomass-derived glucose to 5-hydroxymethylfurfural. The catalyst design improved HMF selectivity and stability, providing insights for the design of new bifunctional solid acid catalysts.
Article
Biochemical Research Methods
Mohor Chatterjee, Jay B. Patel, Spencer T. Stober, Xiaozhou Zhang
Summary: This study genetically modified a Starmerella bombicola strain to produce a high level of hydroxy fatty acid. The synthesized RA was bound with sophorolipid and secreted out of the cell, followed by acid hydrolysis to recover RA. The engineered strain achieved a record production titer of RA. This work highlights a new biocatalysis platform for producing hydroxy fatty acids.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Physical
Mohammad Shahinur Rahaman, Sarttrawut Tulaphol, Anwar Hossain, Jacek B. Jasinski, Shashi B. Lalvani, Mark Crocker, Thana Maihom, Noppadon Sathitsuksanoh
Summary: Aluminum-containing metal-organic frameworks (Al-MOFs) show activity, selectivity, stability, and reusability for glucose isomerization in ethanol. Al-MOFs achieved high fructose selectivity at high glucose conversion, and the catalyst is stable and can be reused at least four times.
Article
Chemistry, Physical
Mohammad Shahinur Rahaman, Sarttrawut C. Tulaphol, Md. Anwar M. Hossain, Matthew Mulvehill, Joshua Spurgeon, Thana Maihom, Noppadon Sathitsuksanoh
Summary: Liquid phase catalytic hydrogenation is crucial for the production of platform chemicals from biomass-derived carbonyl compounds. In this study, the chemical pathway of transfer hydrogenation of benzaldehyde by Hf- and Zr-containing MOF-808 and UiO-66 catalysts was explored. It was found that MOF-808(Hf) exhibited the highest selectivity with a conversion rate of 99%.
MOLECULAR CATALYSIS
(2022)
Article
Chemistry, Physical
Md Anwar Hossain, Tinnakorn Saelee, Sarttrawut Tulaphol, Mohammad Shahinur Rahaman, Thanh Khoa Phung, Thana Maihom, Piyasan Praserthdam, Supareak Praserthdam, Daniel J. Yelle, Noppadon Sathitsuksanoh
Summary: In this study, efficient hydrogenolysis of lignin was achieved using Ru/C catalyst and internal hydrogen. The aliphatic hydroxyl groups in lignin facilitated the cleavage of beta-O-4 bonds and the formation of phenolic monomers. Solvents with high donor numbers enhanced the yield of phenolic monomers. These findings offer a novel approach for the design of lignin isolation processes and solvent systems in biorefineries.
Article
Chemistry, Inorganic & Nuclear
Mohammad Shahinur Rahaman, Sarttrawut Tulaphol, Ashten Molley, Kyle Mills, Md Anwar Hossain, Daniel Yelle, Thana Maihom, Noppadon Sathitsuksanoh
Summary: A catalytic upgrading strategy using metal triflates can efficiently transform benzyl phenyl ether into large phenolic compounds, with potential applications in various fields such as phenol-formaldehyde polymers, diesel/jet fuels, and liquid organic hydrogen carriers.
DALTON TRANSACTIONS
(2021)
Article
Chemistry, Physical
Emtias Chowdhury, Mohammad Shahinur Rahaman, Noppadon Sathitsuksanoh, Craig A. Grapperhaus, Martin G. O'Toole
Summary: Colloidal crystallization using DNA can fabricate highly programmable nanoparticle superstructures with collective plasmonic properties. The 3D plasmonic aggregates from polydisperse gold nanoprisms show excellent performance for applications in sensing, photonics, optoelectronics, and lasing.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)