Article
Biotechnology & Applied Microbiology
Susheng Wang, Rui Wang, Xiuyun Zhao, Gaoqiang Ma, Na Liu, Yuqing Zheng, Jun Tan, Gaofu Qi
Summary: Genetic engineering approaches and changes in biosynthetic pathways can significantly improve the antifungal activity and biosynthesis yield of iturin and fengycin in Bacillus amyloliquefaciens.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Qinlan Jia, Yingying Fan, Shuaishuai Duan, Qiaomei Qin, Yu Ding, Min Yang, Yan Wang, Fengjuan Liu, Cheng Wang
Summary: A rhizospheric bacterium, Bacillus amyloliquefaciens XJ-BV2007, was isolated from tomato fields in Xinjiang, China. It was found to have strong antagonistic effects against Alternaria alternata and can reduce the accumulation of alternaria toxins in tomatoes. The study identified fengycin as an important compound for inhibiting A. alternata. This research is the first to report the efficient control of tomato black spot disease and mycotoxins using B. amyloliquefaciens.
Article
Biochemistry & Molecular Biology
Nandhitha Venkatesh, Claudio Greco, Milton T. Drott, Max J. Koss, Isabelle Ludwikoski, Nina M. Keller, Nancy P. Keller
Summary: This study reveals that chlamydospores can serve as bacterial reservoirs, allowing symbiotic and pathogenic bacteria to survive and propagate in fungi, likely facilitated by bacterial lipopeptides. Furthermore, the colonization of endofungal bacterial communities in chlamydospores suggests a previously unknown ecological role. These findings provide a broader understanding of microbial niches and have significant implications for pathogenic and beneficial bacteria persistence.
Article
Biochemistry & Molecular Biology
Hedong Lu, Ruili Li, Panping Yang, Weibo Luo, Shunxian Chen, Muhammad Bilal, Hai Xu, Chengyuan Gu, Shuai Liu, Yuping Zhao, Chengxin Geng, Li Zhao
Summary: This study investigated the regulatory mechanism of fructose on fengycin biosynthesis using iTRAQ-based proteomic analysis, and found that fructose enhances fengycin biosynthesis by improving metabolic processes and protein synthesis.
Article
Genetics & Heredity
Hedong Lu, Hai Xu, Panping Yang, Muhammad Bilal, Shaohui Zhu, Mengyuan Zhong, Li Zhao, Chengyuan Gu, Shuai Liu, Yuping Zhao, Chengxin Geng
Summary: This study investigated the effect of fructose on fengycin production and its regulation mechanism in B. amyloliquefaciens by transcriptome sequencing. The results showed that fructose increased the transcription of amino acid synthesis, fatty acid metabolism, and energy metabolism, which contribute to the synthesis of fengycin.
Review
Food Science & Technology
Hedong Lu, Panping Yang, Mengyuan Zhong, Muhammad Bilal, Hai Xu, Qihan Zhang, Jiangnan Xu, Naiguo Liang, Shuai Liu, Li Zhao, Yuping Zhao, Chengxin Geng
Summary: This study screened a potential probiotic microbe with broad-spectrum antagonistic activity against food-borne pathogens and identified the antimicrobial compounds it produced. A new Bacillus strain, closely related to Bacillus amyloliquefaciens, was isolated from breeding soil and found to produce effective antimicrobial agents. These agents were identified as fengycin and its isoforms (fengycin A and fengycin B) through analyses. The probiotic activity, antibiotic safety, and viability of the isolated strain were evaluated, indicating that it could be a good choice for biological strains in agricultural commodities and animal feedstuffs.
FOOD SCIENCE & NUTRITION
(2023)
Article
Limnology
Weixiang Liu, Chaomin Sun
Summary: The metabolites of Bacillus subtilis 2H11 from deep-sea sources, especially C-17-fengycin B, showed significant inhibitory effects on the growth of fungal pathogen Fusarium solani in tomato and legume crops. The study used various methods to confirm the antifungal activity of C-17-fengycin B, which also demonstrated good biocompatibility, making it a potential candidate for the development of biocontrol pesticides in the future.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Microbiology
M. Cristina Diez, Cesar Llafquen, Paola Fincheira, Claudio Lamilla, Gabriela Briceno, Heidi Schalchli
Summary: Biosurfactant-producing bacteria were found in contaminated environments, and two strains were selected for their ability to produce biosurfactants. Both strains had good emulsification activities, and their biosurfactant production was independent of biomass.
Article
Biotechnology & Applied Microbiology
Nancy Ley-Lopez, Jose Basilio Heredia, Cesar San Martin-Hernandez, Isabel Cruz-Lachica, Isidro Marquez-Zequera, Raymundo Medina-Lopez, Raymundo Saul Garcia-Estrada
Summary: Cyclic lipopeptides (LPs) have potential applications in agriculture, industry, and pharmaceuticals, but their availability is limited due to low production by bacteria. This study aimed to identify and quantify LP homologues synthesized by Bacillus amyloliquefaciens using different inducers. Ultra-performance liquid chromatography was used to analyze the synthesis of LPs, and the results showed that the highest concentrations were observed in the presence of inactive cells of Coletotrichum sp.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Jaeyoung Choi, Jiyoung Nam, Moon-Hyeong Seo
Summary: Bacillus spp. are important in producing bioactive natural products with agricultural and medical applications, especially the lipopeptides fengycin, iturin, and surfactin with antagonistic activity against other microbes. A novel strain NST6 identified as B. velezensis has 21 putative biosynthetic gene clusters, including those responsible for bacillomycin and surfactin. Analysis shows that gene clusters for iturin families are well conserved in B. amyloliquefaciens species, while clusters for fengycin and surfactin have dynamic distribution at gene level.
Article
Agricultural Engineering
Xin-Yue Chen, Hui-Zhong Sun, Bin Qiao, Chang-Hao Miao, Zheng-Jie Hou, Shu-Jing Xu, Qiu-Man Xu, Jing-Sheng Cheng
Summary: The study found that adding exogenous L-proline, establishing a consortium with recombinant bacteria, and utilizing kitchen waste for hydrolysis can significantly increase the production of lipopeptides by Bacillus amyloliquefaciens HM618. These strategies offer new approaches to improve the production of lipopeptides in an efficient and cost-effective manner.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Chang-Hao Miao, Xiao-Feng Wang, Bin Qiao, Qiu-Man Xu, Chun-Yang Cao, Jing-Sheng Cheng
Summary: The efficiency of producing the broad-spectrum antifungal compound iturin A from food waste can be increased by constructing artificial bacterial consortia incorporating Bacillus amyloliquefaciens and recombinant Bacillus subtilis.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Xiao-Feng Wang, Chang-Hao Miao, Bin Qiao, Shu-Jing Xu, Jing-Sheng Cheng
Summary: In this study, kitchen waste was used as a substrate for the production of fengycin by an artificial consortium containing Bacillus amyloliquefaciens and engineered Pichia pastoris. The engineering strains produced amylase, glucosidase, or lipases. The co-culture of B. amyloliquefaciens and engineering strains promoted the conversion of kitchen waste into fengycin, providing a new strategy for resource utilization.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Microbiology
Maliheh Vahidinasab, Isabel Adiek, Behnoush Hosseini, Stephen Olusanmi Akintayo, Bahar Abrishamchi, Jens Pfannstiel, Marius Henkel, Lars Lilge, Ralf T. Voegele, Rudolf Hausmann
Summary: Bacillus velezensis UTB96 is a promising strain for further research application in the field of agricultural biological controls of fungal diseases, as it can produce higher amounts of antifungal substances and has a stronger inhibitory effect on soybean fungal pathogens.
Article
Biotechnology & Applied Microbiology
Li Ren, Ziqiang Yuan, Tingyu Xie, Daren Wu, Qianjin Kang, Jieming Li, Jian Li
Summary: This study successfully isolated the active substance Epichlicin from Bacillus amyloliquefaciens SJ100001, which effectively controlled the growth of the pathogen Fusarium oxysporum SJ300024 causing cucumber wilt. Epichlicin showed good cell safety and antioxidant properties, making it a potential environmentally friendly biocontrol agent with broad application prospects in medicine.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)