Article
Multidisciplinary Sciences
Zhiquan Wang, Suqing Wu, Chunzhen Fan, Xiangyong Zheng, Wei Zhang, Deyi Wu, Xinze Wang, Hainan Kong
Summary: The study found that the optimal NaOH pre-treatment conditions for enzymatic hydrolysis of wheat straw to enhance reducing sugar yield were NaOH concentration of 1.0%, solid content of 5.0%, and pre-treatment time of 60 minutes. Optimization of parameters such as solid content, enzyme loading, temperature, pH, and hydrolysis time during enzymatic hydrolysis can significantly increase the yield of reducing sugars.
SCIENTIFIC REPORTS
(2021)
Article
Agricultural Engineering
Zhichao Deng, Ao Xia, Yun Huang, Xianqing Zhu, Xun Zhu, Qiang Liao
Summary: This study investigated the relationship between the physicochemical properties and enzymatic hydrolyzability of hydrothermal pretreated lignocellulose through correlation analysis and principal component analysis. The results showed that cellulose content was the major factor affecting carbohydrate conversion, and the initial hydrolysis rate and carbohydrate conversion could be well predicted using principal component analysis.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Neha Srivastava, Rajeev Singh, Manish Srivastava, Asad Syed, Dan Bahadur Pal, Ali H. Bahkali, P. K. Mishra, Vijai Kumar Gupta
Summary: The study utilized mixed fungal consortia for solid state fermentation to enhance cellulase production, and improved the thermal and pH stability of cellulase with NiFe2O4 nanoparticles. The treated cellulase was used for enzymatic hydrolysis of biowaste to biofuels, resulting in significantly higher sugar yield and productivity compared to control.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Jianhong Sun, Yuanfang Deng, Shaohua Li, Wenyong Xu, Guoquan Liu
Summary: This research investigated the enhanced efficiency of enzymatic hydrolysis of wheat straw through freeze-thaw pretreatment and analyzed the changes in physicochemical structure after pretreatment. The study found that cellulose and hemicellulose hydrolysis efficiency was improved, with hemicellulose being more susceptible to pretreatment. The highest hydrolysis efficiency for cellulose and hemicellulose was 57.06% and 70.66%, respectively, achieved at -80℃ for 24 hours and -10℃ for 24 hours, which were 2.23 and 3.13 times higher than the control levels, respectively. The study also observed cracks and holes in the pretreated samples, indicating structural changes. Fourier transform infrared spectroscopy and X-ray diffraction analysis revealed disruptions in the crystalline and amorphous regions and hydrogen bonds. This study provides a physical pretreatment method for enhancing the enzymatic hydrolysis efficiency of wheat straw.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Jiaxin Chen, Xin Wang, Biying Zhang, Yifan Yang, Yangbo Song, Fan Zhang, Bianfang Liu, Yuan Zhou, Yanglei Yi, Yuanyuan Shan, Xin Lu
Summary: The study investigated the optimization of subcritical water pretreatment and high solid hydrolysis for improving the conversion efficiency of bioethanol from wheat straw. Accumulation of lignin in solid residue after enzymatic hydrolysis was found to not affect the efficiency of bioethanol conversion, indicating a promising strategy for utilizing all components of lignocellulosic biomass.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agricultural Engineering
Xinyuan Zhang, Yiqin Wang, Jie Lu, Miaomiao Liu, Wanting Tan, Yi Cheng, Yehan Tao, Jian Du, Haisong Wang
Summary: This paper used five different types of biosurfactants to enhance the saccharification efficiency in the enzymatic hydrolysis process. The addition of sophorolipid increased the enzymatic hydrolysis efficiency by 30.65%, while the combination of sophorolipid and tea saponin further increased the efficiency to 99.56%. These biosurfactants acted synergistically to inhibit enzymatic inactivation and non-productive adsorption of lignin. This study proposes an effective method to improve enzymatic hydrolysis efficiency and reduce enzyme dosage by utilizing biosurfactants.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiumn-Yih Wu, Tzi-Yuan Wang, Hsiou-Yu Ding, Yun-Rong Zhang, Shu-Yuan Lin, Te-Sheng Chang
Summary: Researchers found that an enzyme from Bacillus thuringiensis could convert vitexin into two new compounds with higher aqueous solubility, which enhances the potential applications of vitexin.
Article
Agricultural Engineering
Jie Zhao, Xuxiong Tao, Junfeng Li, Yushan Jia, Tao Shao
Summary: Silencing rice straw with dilute formic acid can reduce the cost of lignocellulosic pretreatment and improve fermentation dynamics and enzymatic hydrolysis. The use of 0.6% formic acid showed the best results in promoting hemicellulose removal and xylose production, leading to increased cellulose convertibility and glucose yield.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Tomohiko Matsuzawa, Akira Watanabe, Takahiro Shintani, Katsuya Gomi, Katsuro Yaoi
Summary: Aspergillus species are closely associated with humanity through fermentation, infectious disease, and mycotoxin contamination of food. This review focuses on the machinery of the xyloglucan degradation in Aspergillus species using glycoside hydrolases, emphasizing the crucial role of cooperative actions of these enzymes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Energy & Fuels
Xinxing Wu, Zhiwei Fan, Shadrick Mwansa, Chen Huang, Qiang Yong
Summary: Exogenous ash penalties hinder the autohydrolysis performance of straw pulp residues. By using H2O2 to oxidize the organic components, the negative charge on the ash surface was increased, reducing the acid buffer and cation exchange capacity of the pretreated wheat straw pulp residue. The enzymatic efficiency of the pretreated residue significantly improved with increasing concentrations of H2O2, leading to increased production of glucose and xylooligosaccharides.
Article
Energy & Fuels
Huanhuan Zhang, Yuanyuan Wang, Ruinan Yang, Zhenli Yan, Zigao Zhao, Chaojun Du, Yujie Chen, Wei Chen, Chun Chang
Summary: This study compared three mild pretreatment methods (formic acid pretreatment, sodium chlorite pretreatment, and alkaline hydrogen peroxide pretreatment) for wheat straw. The results showed that alkaline hydrogen peroxide pretreatment had better enzyme accessibility and higher enzymatic hydrolysis yield, and lignin can be separated from the liquid, improving the process's economics. Furthermore, response surface methodology was used to optimize the enzyme and additive conditions, achieving a maximum enzymatic hydrolysis yield of 94.15%. Fed-batch experiments with high-solid loadings achieved an enzymatic hydrolysis yield of 86.05%. These results contribute to improving the economic feasibility of wheat straw pretreatment and enzymatic hydrolysis process.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Agricultural Engineering
Shihan Hu, Tingwei Zhang, Bo Jiang, Caoxing Huang, Weiqi Wei, Wenjuan Wu, Yongcan Jin
Summary: Sulfonated lignin enhances enzymatic hydrolysis of lignocellulose substrates. Sulfonated polyphenols, such as tannic acid, may have similar effects. Sulfomethylated tannic acids (STAs) with different sulfonation degrees were prepared and investigated for their impact on enzymatic saccharification. STAs significantly promoted the substrate enzymatic digestibility, providing a reliable approach for establishing an efficient lignocellulosic enzyme hydrolysis system.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wei Tang, Xinxing Wu, Caoxing Huang, Zhe Ling, Chenhuan Lai, Qiang Yong
Summary: The research found that humic acids (HA) negatively affected enzymatic hydrolysis efficiency in lignocellulose pretreatment, but introducing metallic cations (Fe3+) to associate with HA can improve enzymatic hydrolysis efficiency. This strategy provides an economically viable means for future biorefinery developments to produce valuable biochemicals, biofuels, and biomaterials.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Maojin Cui, Xiaoyu Li
Summary: This study investigates the effect of additives on enzymatic hydrolysis of lignocellulose biomass and finds that the addition of Triton X-100 and Bovine serum albumin can improve the efficiency of enzymatic hydrolysis, particularly Triton X-100. Kinetic studies show that extracted cellulose has a higher initial enzymatic hydrolysis rate.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Emanuela Calcio Gaudino, Giorgio Grillo, Silvia Tabasso, Livio Stevanato, Giancarlo Cravotto, Kaisa Marjamaa, Ville Pihlajaniemi, Anu Koivula, Nina Aro, Jaana Uusitalo, Jarmo Ropponen, Lauri Kuutti, Pauliina Kivinen, Heimo Kanerva, Alexander Arshanitsa, Lilija Jashina, Vilhelmine Jurkjane, Anna Andersone, Thomas Dreyer, Gerhard Schories
Summary: This paper reports the dominant physical effects of ultrasound (US) pretreatment on improving delignification of wheat straw. The study demonstrates that ultrasound pretreatment can effectively increase the yield of soluble sugars by making the cellulose more susceptible to enzymatic hydrolysis. The results show that ultrasound pretreatment has advantages over steam explosion pretreatment in terms of environmental impact and cost-benefit analysis.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Organic
Olga Gherbovet, Fernando Ferreira, Apolline Clement, Melanie Ragon, Julien Durand, Sophie Bozonnet, Michael J. O'Donohue, Regis Faure
Summary: This study utilized a chemoenzymatic approach to synthesize different ferulate compounds, shortening synthetic routes and enhancing transesterification yields. A novel feruloylated compound was also successfully synthesized. These findings provide new substrate options for characterizing feruloyl esterases.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Mounir Benkoulouche, Akli Ben Imeddourene, Louis-Antoine Barel, Guillaume Le Heiget, Sandra Pizzut, Hanna Kulyk, Floriant Bellvert, Sophie Bozonnet, Laurence A. Mulard, Magali Remaud-Simeon, Claire Moulis, Isabelle Andre
Summary: By engineering carbohydrate-active enzymes, catalysts with altered regioselectivity of glycosylation reaction have been developed, thus expanding the enzyme repertoire for carbohydrate synthesis. Mutants have shown potential for producing pentasaccharides serving as precursors for Shigella flexneri serotypes, demonstrating the impact of mutations on glycosylation specificity.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
David Teze, Jiao Zhao, Mathias Wiemann, Zubaida G. A. Kazi, Rossana Lupo, Birgitte Zeuner, Marlene Vuillemin, Mette E. Ronne, Goran Carlstrom, Jens O. Duus, Yves-Henri Sanejouand, Michael J. O'Donohue, Eva Nordberg Karlsson, Regis Faure, Henrik Stalbrand, Birte Svensson
Summary: This method describes a straightforward strategy involving rapid in silico analysis of protein sequences to identify single-mutant candidates for improving transglycosylation yields. Requiring minimal prior knowledge of the target enzyme, the method is generic and can validate mutations in one enzyme for transposition to others, even distantly related enzymes.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemical Research Methods
Jiao Zhao, Tobias Tandrup, Bastien Bissaro, Sophie Barbe, Jens-Christian N. Poulsen, Isabelle Andre, Claire Dumon, Leila Lo Leggio, Michael J. O'Donohue, Regis Faure
Summary: The study focused on exploring the active site of a GH51 retaining alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus through analysis of two mutants, F26L and L352M. The results showed that the mutant R69H-N216W-L352M displayed excellent transglycosylation activity by inducing changes in the donor and acceptor subsites. Additionally, the central role played by the conserved R69 residue in affecting the T/H partition was reaffirmed.
Article
Biochemistry & Molecular Biology
Jiao Zhao, Jeremy Esque, Isabelle Andre, Michael J. O'Donohue, Regis Faure
Summary: The GH-51 alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus exhibits versatile catalytic properties, with mutants showing increased yields and regioselectivity in the synthesis of furanobiosides. The study demonstrates the potential usefulness of these mutants for glycosynthesis, particularly in the production of biologically-relevant glycoconjugates. Through in-depth characterization, it was found that mutants can maximize the yield of homo-disaccharides by using higher substrate concentrations.
BIOORGANIC CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
U. Javourez, M. O'Donhue, L. Hamelin
Summary: Residual biomass plays a crucial role in the transition towards circular and low fossil carbon economies by providing energy, chemicals, materials, and food products or services. The review presents various waste-to-nutrition pathways and proposes a systematic framework for waste conversion. The study also categorizes different pathways and introduces a multidimensional representation of biomass suitability for nutrition sources.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemical Research Methods
Louise Badruna, Vincent Burlat, Pierre Roblin, Thomas Enjalbert, Guy Lippens, Immacolata Venditto, Michael J. O'Donohue, Cedric Y. Montanier
Summary: This study focuses on investigating carbohydrate-binding modules using the Jo-In biomolecular welding protein pair. By creating recombinant xylanase variants appended to different carbohydrate-binding modules, the research reveals biochemical properties and lays the foundation for understanding the correlation between enzyme activity and substrate properties.
Article
Multidisciplinary Sciences
Philippe Bardou, Sandrine Laguerre, Sarah Maman Haddad, Sabrina Legoueix Rodriguez, Elisabeth Laville, Claire Dumon, Gabrielle Potocki-Veronese, Christophe Klopp
Summary: Functional metagenomics is a classical way to discover genes responsible for specific metabolic functions from uncultured bacteria, with hundreds of sequences already published in public databases. The MINTIA software package enables biologists to easily generate and analyze large metagenomic sequence sets retrieved after activity-based screening, improving research efficiency.
Article
Biotechnology & Applied Microbiology
Doha Elalami, Mouna Aouine, Florian Monlau, Fabienne Guillon, Claire Dumon, Guillermina Hernandez Raquet, Abdellatif Barakat
Summary: This study investigates the impact of extrusion at high solid loading on the properties and enzymatic hydrolysis of corn stover (CS). The results show that extrusion enhances the enzymatic hydrolysis of CS and increases the yields of glucose and xylose. The optimal conditions for solid loading and rotation speed were determined based on the assessment of energy efficiency.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2022)
Review
Biochemistry & Molecular Biology
Ahmed Khamassi, Claire Dumon
Summary: Valuing plant, marine, and algal polysaccharides is crucial for the advancement of the circular bioeconomy. These organic molecules are highly abundant in nature and require a variety of enzymes for degradation due to their complex chemical structures. The understanding and optimization of enzyme synergy for polysaccharide valorization is still limited, but recent research has focused on improving and characterizing this synergy, particularly in the context of plant cell-wall degradation.
ESSAYS IN BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Mareike Monschein, Edita Jurak, Tanja Paasela, Taru Koitto, Vera Lambauer, Mirko Pavicic, Thomas Enjalbert, Claire Dumon, Emma R. Master
Summary: The researchers established a three-dimensional assay called PACER to analyze the migration of enzymes through defined lignocellulose composites. The assay showed that non-catalytic domains of xylanases as well as loosenin-like proteins can enhance the migration of xylanases through cellulose/azo-xylan composites.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Haiyang Wu, Eleni Ioannou, Bernard Henrissat, Cedric Y. Montanier, Sophie Bozonnet, Michael J. O'Donohue, Claire Dumon
Summary: The functional screening of a termite gut metagenomic library identified a xylan-degrading enzyme, Pm25, belonging to GH10 family. Sequence analysis revealed unique domain organization of Pm25, with upstream genes suggesting it as a Xyn10C-like enzyme. The enzyme's multimodularity and CBMs' role were further studied, showing synergistic effects of CBMs on enzymatic activity. These findings enhance understanding of Xyn10C-like proteins in gut bacteria's xylan utilization systems.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
