Article
Biotechnology & Applied Microbiology
Rui Huang, Honglu Liu, Wanwan Zhao, Siqi Wang, Shufang Wang, Jun Cai, Chao Yang
Summary: This study reveals the regulatory roles of AdpA homologs in ε-PL biosynthesis and morphological differentiation in Streptomyces. It demonstrates that AdpA(Sn) promotes ε-PL production by affecting the transcription of its target genes in central metabolism pathways. These findings provide valuable insights into enhancing the production of ε-PL and other valuable secondary metabolites in Streptomyces.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Yongpeng Zhou, Peng Yan, Lei Tang
Summary: This study investigated the effects of the antimicrobial peptide ε-PL on its producer Streptomyces. ε-PL disrupted membrane integrity and increased oxidative stress, leading to cell damage. However, Streptomyces counteracted the injury through self-protective mechanisms, resulting in improved fermentation efficiency of ε-PL.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Qinyu Li, Xiaojia Chen, Yuanjie Wu, Zheng Chen, Yang Han, Peng Zhou, Jiping Shi, Zhijun Zhao
Summary: This study used the CRISPRi system to inhibit the degradation enzyme pldII and found that the inhibition improved the antibacterial effect of ε-PL. The molecular weight distribution of ε-PL changed after inhibition, and the degradation mechanism of ε-PL in S. albulus was revealed through homology modeling and substrate docking of pldII.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hao Yang, Daojun Zhu, Lang Kai, Liang Wang, Hongjian Zhang, Jianhua Zhang, Xusheng Chen
Summary: In this study, a PPK-mediated ATP regeneration system was proposed and developed in S. albulus for the first time, significantly enhancing epsilon-PL production. The study provides an efficient approach to improve the production of not only epsilon-PL but also other ATP-driven metabolites.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Liang Wang, Hao Yang, Mengping Wu, Jianhua Zhang, Hongjian Zhang, Zhonggui Mao, Xusheng Chen
Summary: This study comprehensively analyzed the biosynthetic mechanisms of epsilon-PL in S.albulus WG-608 through integrated physiological, transcriptomic, and proteomic association analysis. The results revealed differential expression of key genes in various metabolic pathways, leading to increased carbon flux into L-lysine biosynthesis and epsilon-PL production. Furthermore, changes in the regulation of DNA replication, transcription, and translation, as well as quorum sensing, were observed, potentially contributing to environmental adaptability and epsilon-PL biosynthesis. Overexpression of the ppk gene and addition of polyP(6) further enhanced epsilon-PL production.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xidong Ren, Yan Chen, Yangzi Guo, Kunpeng Li, Chenying Wang, Xinli Liu
Summary: In this study, the dynamic responses of Streptomyces albulus QLU58 and its acid-tolerant mutants to autoacidification were investigated at the physiological and transcriptional levels. The results showed that autoacidification disturbed cell growth, e-PL production, cell respiratory activity, and intracellular pH (pH(i)) homeostasis. Acid-tolerant mutants exhibited better performances during autoacidification, which was also confirmed by the improved transcription levels of related genes.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Jiaolong Fu, Cong Li, Xin Ju, Jing Bai, Yunfeng Zhou, Yi Zhang, Yue Wang, Zilong Sun, Cuiying Hu, Liangzhi Li, Lilian Ji
Summary: This study investigated the production of epsilon-poly-L-lysine (epsilon-PL) from cassava bagasse hydrolysate by Streptomyces albulus US3-18 and found that the strain exhibited high productivity and tolerance to volatile byproducts. The study provides a reference for efficient production of epsilon-PL from low-cost agricultural residues.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Microbiology
Yongjuan Liu, Kaifang Wang, Long Pan, Xusheng Chen
Summary: In this study, a better ε-PL producing strain was generated through genome shuffling on strains obtained by ribosome engineering. The mutant strain SG-86 produced 144.7% more ε-PL than the parent strain M-Z18. Illumina sequencing was used to map the genomic changes leading to the improved phenotype, revealing a smaller genome size, metabolic pathway changes, and gene deletions in the mutant strain. This study is of great significance for improving fermentation performance and providing theoretical guidance for metabolic engineering of ε-PL producing strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu Du, Pengcheng Huang, Weifeng Jin, Chang Li, Jiehong Yang, Haitong Wan, Yu He
Summary: This study optimized the extraction/purification process of multiple components using the entropy weight method combined with design methods, with Astragalus saponins as a case study. By screening significant factors through PBD and CCD, the optimal purification conditions were determined, leading to a successful practical application of the method to extract and purify natural products.
Article
Chemistry, Multidisciplinary
Jie Yu, Danwei Cen, Yongcheng Chen, Hailan Zhao, Mengyue Xu, Sulan Wu, Shuo Wang, Qiao Jin, Ting Shen
Summary: Antibiotic resistance poses a significant threat to public health, and improving the therapeutic efficacy of conventional antibiotics is an effective solution. By conjugating the positively charged epsilon-poly-l-lysine to erythromycin, the modified EPL-EM showed enhanced antibacterial activity and reduced drug resistance development. Furthermore, EPL-EM exhibited excellent biocompatibility without inducing hemolysis and cytotoxicity.
Article
Chemistry, Applied
Cong Wang, Hui Cao, Lingyun Jia, Wenli Liu, Pengtao Liu
Summary: This study designed an aerogel material using TOCNF/ε-PL network and citric acid as the cross-linking agent. Experimental results showed that the aerogel had good mechanical properties, antibacterial effects, biodegradability, and blood compatibility, indicating great potential applications in medical and food packaging fields.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Hao Wu, Shuqian Hu, Chunling Nie, Jiahui Zhang, Huafeng Tian, Weicheng Hu, Ting Shen, Jianguo Wang
Summary: A novel method was described for synthesizing antibacterial cellulose beads, which exhibited excellent antimicrobial activity and biodegradability, showing great potential for use in food and environmental applications.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Xinglin Jiang, Yulia Radko, Tetiana Gren, Emilia Palazzotto, Tue Sparholt Jorgensen, Tao Cheng, Mo Xian, Tilmann Weber, Sang Yup Lee
Summary: epsilon-Poly-L-lysine, a potent antimicrobial produced from Streptomyces, is widely distributed in cheese and human skin bacteria, suggesting its role as a bioactive secondary metabolite in cheese and skin ecosystems. This discovery raises the possibility of epsilon-Poly-L-lysine naturally existing in cheese and on human skin. The gene responsible for producing epsilon-Poly-L-lysine is found in various coryneform actinobacteria isolated from cheese and human skin, indicating that its production is not limited to filamentous actinobacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Shu Li, Yao Yao, Shengjie Hu, Junru Chen
Summary: A new marine bacterium Bacillus pumilus was discovered to produce short-chain gamma-PAB, which exhibited strong inhibitory activities against yeasts but weaker activities against bacteria. Through pH control strategy, the production of gamma-PAB was significantly enhanced, suggesting a potential new metabolic mechanism in B. pumilus. This discovery represents a new finding in the field of homopolymer research.
CURRENT MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Chunling Nie, Ting Shen, Weicheng Hu, Qin Ma, Jiahui Zhang, Shuqian Hu, Huafeng Tian, Hao Wu, Xiaogang Luo, Jianguo Wang
Summary: In this study, cellulose beads grafted with epsilon-poly-L-lysine were prepared through chemical reactions. The grafted beads showed strong antibacterial activities and biodegradability, indicating potential applications in water purification and food treatment fields.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Chemical
Manjusha A. Thombare, Dinesh Kalaga, Sandip B. Bankar, Rahul K. Kulkarni, Satchidanand R. Satpute, Prakash Chavan
PARTICULATE SCIENCE AND TECHNOLOGY
(2020)
Article
Food Science & Technology
Nirali Shah, Anne Usvalampi, Sandeep Chaudhary, Tuulikki Seppanen-Laakso, Sandesh Marathe, Sandip Bankar, Rekha Singhal, Salem Shamekh
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Hakim Hebal, Arno Parviainen, Sasikala Anbarasan, He Li, Laura Makkonen, Sandip Bankar, Alistair W. T. King, Ilkka Kilpelainen, Said Benallaoua, Ossi Turunen
PROCESS BIOCHEMISTRY
(2020)
Article
Agricultural Engineering
Bruna Pratto, Vijaya Chandgude, Ruy de Sousa Junior, Antonio Jose Goncalves Cruz, Sandip Bankar
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Engineering, Chemical
Manisha A. Khedkar, Pranhita R. Nimbalkar, Shashank G. Gaikwad, Prakash Chavan, Sandip B. Bankar
SEPARATION AND PURIFICATION TECHNOLOGY
(2020)
Article
Thermodynamics
Vijaya Chandgude, Teemu Valisalmi, Juha Linnekoski, Tom Granstrom, Bruna Pratto, Tero Eerikainen, German Jurgens, Sandip Bankar
Summary: This study successfully improved the product titer and yield of ABE fermentation production by combining reducing agents and a controlled feeding strategy, leading to efficient utilization of glucose and significant enhancement in solvent production.
ENERGY CONVERSION AND MANAGEMENT
(2021)
Article
Thermodynamics
Manisha A. Khedkar, Satchidanand R. Satpute, Sandip B. Bankar, Prakash Chavan
Summary: The study aimed to investigate the efficacy of silver ion chromatography in selectively separating unsaturated fatty acids. The first part of the study focused on adsorption of oleic acid using R-Ag+ resin, showing that R-Ag+ had the highest adsorption capacity for oleic acid at 303 K. The study also found that the adsorption of oleic acid on R-Ag+ resin was spontaneous and exothermic.
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2021)
Article
Chemistry, Multidisciplinary
Shrikant A. Survase, Pranhita Nimbalkar, German Jurgens, Tom Granstrom, Prakash Chavan, Sandip Balasaheb Bankar
Summary: This study evaluated the impact of lignosulfonate on ABE production and proposed a technique to overcome lignosulfonate inhibitory action. Addition of soy meal was shown to enhance Clostridium acetobutylicum survival and increase butanol concentration during fermentation. The transcriptional analysis revealed upregulation of key genes in clostridial metabolic pathways in the presence of soy meal.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Food Science & Technology
Somnath Basak, Rahul Venkatram, Rekha S. Singhal
Summary: Molecular imprinting is gaining significant attention in food analysis for its ability to extract and analyze various analytes in complex food matrices. It has potential applications in food safety, quality control, and sample clean-up, offering a promising solution for analyzing and detecting contaminants in food.
Review
Food Science & Technology
Somnath Basak, Snehasis Chakraborty, Rekha S. Singhal
Summary: A closer look at traditional Indian foods reveals their efficacy and wisdom in utilizing regional resources and achieving perfection in processing. Engineering discussion and analysis of these foods is important in the era of convenience foods and mechanization. This review explores the critical engineering properties of Indian traditional foods, including heat and mass transfer processes, and calls for further research in this area.
Article
Green & Sustainable Science & Technology
Abhinav Sharma, Aratrika Ray, Rekha S. Singhal
Summary: This study compared the co-extraction of turmeric and dried coconut shreds through supercritical fluid extraction (SFE) and conventional solvent extraction using n-hexane. The optimized parameters for SFE resulted in a yield of 45 mg/g and a total curcuminoid content of 723 μg/g. The SFE co-extracts exhibited various biological activities, including antioxidant, anti-inflammatory, antibacterial, and anti-cancerous effects.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Multidisciplinary
Nahla Osmanbegovic, Vijaya Chandgude, Sandip Bankar, Marjatta Louhi-Kultanen
Summary: The suspension freeze crystallization method was used to concentrate aqueous 1-butanol solutions, synthetic acetone-butanol-ethanol (ABE) solutions, and ABE fermentation broth, which required less energy compared to evaporation for water removal. Equimolar aqueous ABE solutions with a total molality range of 0-5.05 mol/kg(water) were found to be ideal based on the obtained freezing point depression. Suspension freeze crystallization separated 1-butanol from the mother liquor, resulting in a water-enriched phase and a 1-butanol-enriched phase. Higher subcooling degrees and initial water content in the feed solutions led to higher ice yield values.
CRYSTAL GROWTH & DESIGN
(2023)
Article
Food Science & Technology
Aratrika Ray, Abhinav Sharma, Rekha S. Singhal
Summary: This study aimed to fabricate a porous hydrogel by crosslinking purified subabul galactomannans (SG) and kappa-carrageenan (kappa C) with whey protein isolate (WPI). The hydrogel showed high foam overrun and minimal foam drainage at a specific composition. The resulting SG and WPI incorporated porous hydrogel (SGWP) exhibited excellent stability and water uptake rate at elevated temperature and neutral pH, suggesting its potential applications in various industries and as a matrix for bioactive delivery.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Food Science & Technology
Shilpa B. Jana, Rekha S. Singhal
Summary: This study presents an approach to reduce the bitterness of neem juice by adding it to sugar and preparing co-crystallized sugar cubes. The sugar cubes contain zinc, which effectively reduces the bitterness and stabilizes the antioxidant properties of neem.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Biochemistry & Molecular Biology
Kapil Rai, Narayansing Chhanwal, Nirali N. Shah, Rekha S. Singhal
Summary: The study focused on encapsulating ginger oleoresin in a sucrose matrix through co-crystallization, resulting in changes in the crystallinity of sucrose and improved encapsulation efficiency. The research also found that [6]-gingerol was the most stable among the gingerols studied, with optimal storage conditions identified at 25 degrees C and 33% relative humidity for ginger-flavored sugar cubes.
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)