Article
Biotechnology & Applied Microbiology
Nguyen Hieu Nghia, Yoichi Kumada, Michimasa Kishimoto, Jun-ichi Horiuchi
Summary: The study demonstrated the stabilized production of anti-CRP single-chain Fv (scFv) antibody using recombinant E. coli in prolonged dissolved oxygen (DO)-stat fed-batch culture by focusing on the supply of nitrogen sources. By optimizing the ratio of glucose and yeast extract (YE) in the feeding medium, the proteolysis of scFv was successfully prevented and the antibody production was enhanced significantly.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Nguyen Hieu Nghia, Yoichi Kumada, Michimasa Kishimoto, Jun-ichi Horiuchi
Summary: The study successfully increased the production and solubility of anti-CRP scFv using the DO-stat fed-batch culture, while effectively suppressing the formation of inclusion bodies.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Agricultural Engineering
Chunli Yu, Hui-Ping Wang, Tengsheng Qiao, Yongteng Zhao, Xuya Yu
Summary: The study found that intermittent succinic acid feeding method in fed-batch culture significantly promoted the production of astaxanthin and lipids in Haematococcus pluvialis, resulting in the highest productivity levels.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Youzhou Liu, Yaqiu Zhou, Junqing Qiao, Wenjie Yu, Xiayan Pan, Tingting Zhang, Yongfeng Liu, Shi-En Lu
Summary: The study found that phenazine-1-carboxylic acid (PCA) is the main antimicrobial compound produced by bacterial strain YL-1 in liquid medium, enhancing the inhibitory effects against Acidovorax citrulli by increasing the accumulation of reactive oxygen species and the activity of catalase.
Article
Biochemistry & Molecular Biology
W. Sriphochanart, W. Skolpap
Summary: The optimal culture conditions for L-lactic acid production by Lactiplantibacillus plantarum TBRC 9667 were investigated, with a temperature shift from 25 to 35 degrees C after 3 hours found to be the most suitable condition. Fed-batch experiments showed a 1.5-fold improvement in L-lactic acid production compared to suboptimal batch culture, highlighting the potential of integrated temperature shift and off-line feeding strategies.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Pornkamol Unrean, Verawat Champreda
Summary: This study developed a bioprocess for upgrading lignin into bioplastics using a novel strain of Pseudomonas fulva for microbial bioconversion. The optimized fed-batch fermentation of lignin-to-PHA resulted in significantly higher PHA concentration and content compared to batch process. The established lignin to PHA conversion provides a platform for developing integrated lignin bioprocessing and promoting cost-effective biorefineries.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Biotechnology & Applied Microbiology
Maciej W. Guzik, Gearoid F. Duane, Shane T. Kenny, Eoin Casey, Pawel Mielcarek, Magdalena Wojnarowska, Kevin E. O'Connor
Summary: The study focused on modeling and optimizing the production process of medium chain length polyhydroxyalkanoate (mcl-PHA) by Pseudomonas putida KT2440 using a synthetic fatty acid mixture (SFAM). Different feeding strategies were developed and tested to achieve high cell dry weight and PHA content, with successful operation at both 5 l and 20 l scales.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Shih-Wei Yen, Dillirani Nagarajan, Wei-Hsin Chen, Duu-Jong Lee, Jo-Shu Chang
Summary: In this study, Aurantiochytrium sp. CJ6 was cultivated with heterotrophic fermentation using sorghum distillery residue (SDR) hydrolysate as the feedstock, without the addition of nitrogen sources. Optimal operating parameters were determined, and batch cultivation achieved high biomass concentration and astaxanthin content. Continuous-feeding fed-batch (CF-FB) fermentation further increased biomass concentration and astaxanthin production. This study demonstrates the potential of using SDR as a sustainable feedstock for the production of high-value products like astaxanthin.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
X. L. Jiang, M. Y. Jin, X. C. Piao, C. R. Yin, M. L. Lian
Summary: This study investigated the effects of four feeding medium factors on the culture efficiency of Oplopanax elatus adventitious roots, and identified the optimal conditions for sucrose concentration, salt strength, phosphorus, and nitrogen concentration. The findings provide a reference for further improving the fed-batch culture system of O. elatus adventitious roots. The application of the analytic hierarchy process method lays a foundation for scientifically and reliably analyzing data with multiple indexes in the field of plant cell culture.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Kaiquan Liu, Zhenghua Li, Xiaoli Liang, Yanpeng Xu, Yufei Cao, Ruiming Wang, Piwu Li, Ling Li
Summary: Phenazine-1-carboxylic acid (PCA) is a biologically active substance that can prevent and control crop diseases. A Pseudomonas chlororaphis strain, Lzh-T5, was used as the starting strain and genetic modifications were made to enhance PCA production. Through various strategies, PCA production was increased to 10,653 mg/L, indicating the potential of P. chlororaphis Lzh-T5 as an efficient cell factory for biologically active substances.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yueh-Hao Ronny Hung, Michael Chae, Dominic Sauvageau, David C. Bressler
Summary: This study developed two adapted feeding strategies to improve ethanol productivity in fed-batch fermentation. The implementation of these strategies led to a 20% increase in ethanol productivity and a final ethanol content of 95-104g/L. These findings provide novel approaches to enhance sugar delivery and ethanol productivity in the ethanol industry.
Article
Chemistry, Multidisciplinary
Daniel Tinoco, Rui de Paula Vieira de Castro, Douglas Teixeira, Francisco de Assis Beltrao Junior, Eduardo de Oliveira Junior, Paulo Luiz de Andrade Coutinho, Denise Maria Guimaraes Freire
Summary: Bio-based 2,3-BDO production can be improved through fed-batch fermentation and nutritional control. The relationship between different feeding profiles and nitrogen sources for high-yield 2,3-BDO production by Paenibacillus peoriae NRRL BD-62 was investigated in this study. Constant fed-batch fermentation using glucose and yeast extract (YE) achieved 28.6 g L-1 2,3-BDO at an initial C/N of 8.5 g g(-1), while reduction was observed at an initial C/N of 18.5 g g(-1). Pulse feeding assays showed no statistical differences. Feeding with NH4Cl instead of YE resulted in higher 2,3-BDO titer and yield, but also led to glucose accumulation due to nutritional limitation.
REACTION CHEMISTRY & ENGINEERING
(2023)
Article
Microbiology
Xiaoyan Chi, Yanhua Wang, Jing Miao, Wei Wang, Yanyang Sun, Zhifen Yu, Zhibin Feng, Shiwei Cheng, Lijuan Chen, Yihe Ge
Summary: The study showed that the transcriptional regulator gene eppR plays a crucial role in the production of phenazine-1-carboxylic acid in Pseudomonas chlororaphis G05, but has no effect on pyrrolnitrin biosynthesis.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Yun-Ling Fang, Ying Cui, Lian Zhou, Chitti Thawai, Tatheer Alam Naqvi, Hong-Yan Zhang, Ya-Wen He
Summary: The H-NS family member protein MvaU in Pseudomonas aeruginosa regulates PCA biosynthesis by self-regulation, repressing the expression of the phz2 cluster and consequently reducing PCA production. MvaU binds directly to the promoter of the phz2 cluster, demonstrating a new regulatory pathway for PCA biosynthesis in PA1201.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Biology
Yu-Xuan Li, Sheng-Jie Yue, Yi-Fan Zheng, Peng Huang, Yan-Fang Nie, Xiang-Rui Hao, Hong-Yan Zhang, Wei Wang, Hong-Bo Hu, Xue-Hong Zhang
Summary: This study developed a cost-effective minimal medium for efficient synthesis of phenazine compounds. By optimizing the composition of the medium, the production of phenazine compounds by Pseudomonas chlororaphis significantly increased while the cost decreased.
Article
Biochemistry & Molecular Biology
Zheng Wang, Xianqing Huang, Yujie Liu, Guohuan Yang, Yang Liu, Xuehong Zhang
MOLECULAR MICROBIOLOGY
(2017)
Article
Microbiology
Yang Liu, Zheng Wang, Muhammad Bilal, Hongbo Hu, Wei Wang, Xianqing Huang, Huasong Peng, Xuehong Zhang
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Yujie Liu, Huimin Shi, Zheng Wang, Xianqing Huang, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Microbiology
Guohao Wang, Xianqing Huang, Sainan Li, Jiaofang Huang, Xue Wei, Yaqian Li, Yuquan Xu
JOURNAL OF BACTERIOLOGY
(2012)
Article
Microbiology
Xue Wei, Xianqing Huang, Lulu Tang, Daqiang Wu, Yuquan Xu
JOURNAL OF BACTERIOLOGY
(2013)
Article
Multidisciplinary Sciences
Xue-Jie Jin, Hua-Song Peng, Hong-Bo Hu, Xian-Qing Huang, Wei Wang, Xue-Hong Zhang
SCIENTIFIC REPORTS
(2016)
Article
Biotechnology & Applied Microbiology
Huimin Shi, Xianqing Huang, Zheng Wang, Yejun Guan, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Lingyu Wu, Zheng Wang, Yejun Guan, Xianqing Huang, Huimin Shi, Yujie Liu, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Zheng Wang, Xianqing Huang, Malik Jan, Deyu Kong, Jingwen Pan, Xuehong Zhang
Summary: The study revealed that the biosynthesis of antimicrobial secondary metabolites in Pseudomonas protegens H78 is regulated by the RNA chaperone Hfq and the catabolite repression control (Crc) protein. Hfq has a more extensive and intensive regulatory capacity compared to Crc, positively controlling gene clusters involved in secondary metabolism, secretion systems, and compound degradation.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zheng Wang, Xianqing Huang, Malik Jan, Deyu Kong, Wei Wang, Xuehong Zhang
Summary: Lon mutation significantly improves the antimicrobial activity of Pseudomonas chlororaphis HT66 strain by degrading PhzI protein, thus regulating the biosynthesis of PCN. Lon forms a new autoregulatory feedback circuit to modulate its own degradation and is responsible for the negative regulation of PCN biosynthesis and phzABCDEFG transcription mediated by PhzI in HT66.
MOLECULAR MICROBIOLOGY
(2021)
Article
Environmental Sciences
Zheng Wang, Xianqing Huang, Chenxi Nie, Tao Xiang, Xuehong Zhang
Summary: In P. protegens H78, Lon protease negatively regulates Plt biosynthesis by decreasing the abundance and stability of GacA and PltR proteins.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Shuqi Guo, Yining Wang, Bona Dai, Wei Wang, Hongbo Hu, Xianqing Huang, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Xuemei Shen, Zheng Wang, Xianqing Huang, Hongbo Hu, Wei Wang, Xuehong Zhang
Article
Biotechnology & Applied Microbiology
Qiang Zhao, Hong-Bo Hu, Wei Wang, Xian-Qing Huang, Xue-Hong Zhang
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2017)
Article
Microbiology
Xianqing Huang, Zheng Wang, Yujie Liu, Xuehong Zhang
GENOME ANNOUNCEMENTS
(2017)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)