Article
Biochemistry & Molecular Biology
Helena D. Tjornelund, Jesper Vind, Jesper Brask, John M. Woodley, Gunther H. J. Peters
Summary: This study investigates the molecular mechanism of the specific activity of Candida antarctica lipase B (CALB) in low-water environments using molecular dynamics simulations. Based on experiments and simulations, four criteria influencing the performance of the enzyme in organic media were proposed and it was shown that specific mutants can resist changes in water activity.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Tuan Kiet Tran, Mamata Singhvi, Ji Wan Jeong, Pritam Kumar Dikshit, Hak-Ryul Kim, Ching T. Hou, Beom Soo Kim
Summary: Cell-free synthesis has been adopted for improved production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid, showing faster production rates and higher product concentrations compared to conventional whole cell culture methods. Optimal conditions for DOD production were found to be at 27 degrees C and pH 8.0, with galactose and sodium glutamate identified as the most effective carbon and nitrogen sources in pre-culture. The study also confirmed the presence of enzymes related to DOD production in the cell-free supernatant, demonstrating the potential benefits of cell-free DOD production for large-scale production.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Pathikrit Saha, Lalit Goswami, Beom Soo Kim
Summary: In this study, a green and sustainable strategy was used to synthesize a novel biobased polymer with excellent mechanical and thermal properties through microbial conversion and modification. The synthesized polymer shows great potential for a wide range of applications.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Green & Sustainable Science & Technology
Hyunjun Ko, Mi-Jin Kim, Hyun-Jin Kim, Jin Kang, Ho-Yeon Lee, Jin Hyuk Lee, Jung-Hoon Bae, Bong Hyun Sung, Jung-Hoon Sohn
Summary: Food waste oil is a suitable raw material for biodiesel production, but water inhibits the reaction. By developing a mutant lipase CALB1422, ester synthesis can be catalyzed in the presence of water, resulting in higher conversion rates and yields of biodiesel from waste oil.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Polymer Science
Joon Young Oh, Yu-Ri Oh, Dong-Myung Kim, Gyeong Tae Eom, Jae Kwang Song
Summary: Plastic waste causes environmental pollution, and enzymatic depolymerization is an environmentally friendly method for plastic recycling. In this study, a highly active polycaprolactone (PCL)-depolymerizing enzyme was screened and produced, which can efficiently degrade PCL and produce a large quantity of degradation products.
POLYMER DEGRADATION AND STABILITY
(2022)
Article
Chemistry, Physical
Koen Adriaensen, Jannick Vercammen, Cedric Van Goethem, Kumar Varoon Agrawal, Dirk De Vos
Summary: In this study, a chemoenzymatic dynamic kinetic resolution (DKR) method for beta-amino esters was established through the evaluation of metal-based heterogeneous catalysts for racemization and enzyme catalysts for kinetic resolution. By selecting two promising racemization catalysts, the researchers established a relationship between catalyst characteristics and reaction selectivities, and achieved efficient DKR with excellent enantioselectivity.
CATALYSIS SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ji Wan Jeong, Mamata Singhvi, Beom Soo Kim
Summary: This study successfully produced 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) using extracellular enzymes from Pseudomonas aeruginosa. The concentration and productivity of DOD were improved compared to conventional whole cell culture method. Glycerol was found to be a better carbon source for DOD production than glucose or crude glycerol. A fed-batch process with the addition of surfactant and enzyme concentrate further increased DOD production, achieving the highest reported DOD concentration (27.5 g/L) using extracellular enzymes.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Martha Martin del Campo, Osvaldo Gomez-Secundino, Rosa M. Camacho-Ruiz, Juan C. Mateos Diaz, Marcelo Muller-Santos, Jorge A. Rodriguez
Summary: The effects of different concentrations of Hofmeister salts on the hydrolytic activity and secondary structure of CALB were investigated. The results showed that high salt concentrations enhanced the activity of CALB on specific substrates and induced structural changes after long-time incubation. These findings have important implications for understanding the catalytic mechanism of enzymes in high-salt environments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Food Science & Technology
Corina Vasilescu, Cristina Paul, Simona Marc, Iosif Hulka, Francisc Peter
Summary: This study reports the synthesis of a hybrid sol-gel material based on organically modified silanes (ORMOSILs) with epoxy functional groups and its application in the stabilization of lipase type B from Candida antarctica (CalB) through sol-gel entrapment. The immobilization parameters were optimized, resulting in a matrix with excellent properties for biocatalytic esterifications. Characterization of the immobilized biocatalyst and correlation with the sol-gel matrix properties were accomplished through various techniques. The immobilization increased the operational and thermal stability of lipase, making it suitable for solventless synthesis applications.
Article
Chemistry, Physical
Judit E. Puskas, Gayatri Shrikhande, Kristof Molnar
Summary: This study investigated the synthesis of fluorescein-diacrylate (FL-DA) and four-functional fluoresceins (FL) through the Michael addition of secondary amines to FL-DA. FL-DA was successfully synthesized at a scale of 100 g. Diallyl amine (DAA) rapidly reacted with FL-DA, while diethanolamine (DEA) had a longer reaction time and was unstable. The addition reactions of aminodiols with longer carbon chains and diethyl-amino-diacetate (DIDA) were slower but could be accelerated using Candida antarctica lipase B (CALB) as a biocatalyst. The structure of tetrafunctional fluoresceins was confirmed by 13C and 1H NMR, and high resolution Mass Spectrometry (HRMS) demonstrated the formation of highly pure tetraallyl- and tetraethylacetate-fluoresceins. These multifunctional fluoresceins show promise for the synthesis of imaging agents for various applications.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemistry & Molecular Biology
Zeping Lu, Mei Chen, Tongtong Jin, Binbin Nian, Yi Hu
Summary: Ionic liquids (ILs) are used as chemical modifiers to improve the efficiency, activity, and stability of enzymes. This study investigated the impact of ILs with different chain lengths on the enzymatic properties of Candida antarctica lipase B (CALB) and found that ILs significantly influenced CALB's absorption orientation. Molecular dynamics simulations showed that ILs with suitable chain lengths formed strong interactions with CNTs and ILs, stabilizing CALB's open state and improving its activity and stability. This study provides insights into the design of green modifiers for better immobilized enzymes and lays the groundwork for future research on IL modifiers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Jose Jesus Guzman-Mendoza, David Chavez-Flores, Silvia Lorena Montes-Fonseca, Carmen Gonzalez-Horta, Erasmo Orrantia-Borunda, Blanca Sanchez-Ramirez
Summary: The study utilized Candida antarctica lipase to functionalize insulin onto the surface of carbon nanotubes, achieving a higher efficiency and greener process compared to previous methods. This enzymatic esterification method can be applied to enhance cellular-specific release of proteins.
Article
Biochemistry & Molecular Biology
Shanxiu Ming, Shuyi Li, Zhe Chen, Xujun Chen, Feifei Wang, Shaonan Deng, Krystian Marszalek, Zhenzhou Zhu, Wenxiang Zhang, Francisco J. Barba
Summary: Traditional enzymatic esterification methods have limitations in improving the bioavailability of flavonoids, while the novel BLIMs technology shows great potential in enhancing compound utilization. Among the three BLIMs, GA/CAL-B@PDA/PES performs the best with high enzyme activity and loading, as well as stable performance under various environmental conditions.
Article
Multidisciplinary Sciences
Katarzyna Swiderek, Susana Velasco-Lozano, Miquel A. Galmes, Ion Olazabal, Haritz Sardon, Fernando Lopez-Gallego, Vicent Moliner
Summary: Biocatalysis plays a crucial role in plastic recycling. However, the understanding of the molecular mechanisms governing the catalytic performance of plastic-degrading enzymes is limited, hindering the development of more efficient enzyme-based technologies. This study investigates the hydrolysis of PET-derived diesters and PET trimers using CALB and provides insights into CALB's regioselectivity. The findings enable selective hydrolysis of BHET and offer potential applications in the valorization of PET by-products.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Honghai Wang, Wenda Yue, Shuling Zhang, Yu Zhang, Chunli Li, Weiyi Su
Summary: The study demonstrated a simple and highly effective strategy for stabilizing enzymes embedded inside silica xerogels by a polydopamine (PDA) coating. The modified silica xerogels showed improved loading ability, retention of enzyme activities, as well as mechanical properties, thermal stability and reusability. These advantages make the new hybrid material a promising support for immobilized enzymes.
Review
Agricultural Engineering
Sumit Kumar, Arun K. Dangi, Pratyoosh Shukla, Debabrat Baishya, Sunil K. Khare
BIORESOURCE TECHNOLOGY
(2019)
Article
Environmental Sciences
Jasneet Grewal, Rameshwar Tiwari, S. K. Khare
WASTE AND BIOMASS VALORIZATION
(2020)
Article
Biotechnology & Applied Microbiology
Sam Joy, Pattanathu K. S. M. Rahman, Sunil K. Khare, Shashi Sharma
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2019)
Article
Agricultural Engineering
Syeda Warisul Fatima, Rameshwar Tiwari, Sunil K. Khare
BIORESOURCE TECHNOLOGY
(2019)
Article
Environmental Sciences
Ayesha Sadaf, Razi Ahmad, Achraf Ghorbal, Walid Elfalleh, Sunil Kumar Khare
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Sam Joy, Sunil K. Khare, Shashi Sharma
PROCESS BIOCHEMISTRY
(2020)
Article
Environmental Sciences
Amrik Bhattacharya, Ayesha Sadaf, Swati Dubey, Rajesh P. Singh, Sunil Kumar Khare
Summary: In this study, bacterial nanocellulose (BNC) was successfully produced using Komagataeibacter xylinus strain SGP8, and characterized using SEM, XRD, and TGA. The BNC exhibited nanofibrillar structure and high crystallinity index, showing excellent adsorption performance for Cd (II).
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Food Science & Technology
Tanya Nagpal, Shahenvaz Alam, Sunil K. Khare, Santosh Satya, Shivani Chaturvedi, Jatindra K. Sahu
Summary: In the study, it was found that Psidium guajava leaves extract inhibited lipid oxidation and decreased the formation of acrylamide in deep-fried food, providing oxidative stability.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Review
Food Science & Technology
Tanya Nagpal, Jatindra K. Sahu, Sunil K. Khare, Khalid Bashir, Kulsum Jan
Summary: Trans fats are desired by the edible oil industry for their functionality, but they have adverse effects on human health and nutrition in food. Global regulatory actions have been taken to eliminate trans fats from the food supply, with the World Health Organization leading a REPLACE action plan to achieve this by 2023. Various technologies have been developed to reduce trans fats in food, with interesterification and oleogelation emerging as promising methods.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Shahenvaz Alam, Shine Augustine, Tarun Narayan, John H. T. Luong, Bansi Dhar Malhotra, Sunil K. Khare
Summary: The study demonstrated the rapid and simple electroanalysis of acrylamide using a gold electrode modified with gold nanoparticles and dithiothreitol, which exhibited enhanced sensitivity and selectivity. Differential pulse voltammetry was used to detect ACR with low limits of detection and quantitation, and a wide linear range. The sensor's estimation of ACR levels in potato chips and coffee samples was consistent with high-performance liquid chromatography results.
Article
Biotechnology & Applied Microbiology
Amrik Bhattacharya, S. K. Khare
Summary: This study investigates the remediation potential of Bacillus cereus EMB20's β-lactamase preparation for the removal of Ampicillin (Amp) and other beta-lactam antibiotics. Results show that the enzyme is effective in completely remediating Amp at different concentrations within an hour, and its activity is not limited to Amp but also extends to other antibiotics.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Polymer Science
Sunny Malik, Ankita Maurya, Sunil Kumar Khare, Kinshuk Raj Srivastava
Summary: Plastic materials are difficult to degrade in the open environment, leading to the infiltration of plastic wastes into natural resources. The current disposal methods are inefficient, and recycling is challenging due to the mixed composition of plastic waste. This research analyzed previously published reports on the biodegradation of different plastic types and found that microbial degradation patterns follow the chemical classification of plastic types. The study suggests potential genera for the bioremediation of mixed plastic waste.
Article
Materials Science, Biomaterials
Indranil Mondal, Syeda Warisul Fatima, Swati Priya, Sagar Sengupta, Sunil Kumar Khare, Ritu Kulshreshtha
Summary: A novel nanostructure called TGNFs has been developed for efficient delivery of anti-miR-210 to glioblastoma cells, resulting in decreased proliferation and migration and increased apoptosis. This method shows potential as a delivery agent in miRNA inhibition therapy.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Nikky Goel, Moumita Ghosh, Deepti Jain, Rajeshwari Sinha, Sunil Kumar Khare
Summary: Biofilm-associated disorders, caused by Pseudomonas aeruginosa, are difficult to treat due to their resistance to the host's immune system and stress factors. In this study, a rare halophilic actinobacteria, Nocardiopsis lucentensis EMB25, was isolated and its secondary metabolites were found to inhibit and eradicate P. aeruginosa biofilm. The secondary metabolites target the quorum sensing pathway and bind to LasR and RhlR. The downregulation of various QS system genes further confirmed the action of secondary metabolites on LasR and RhlR, making actinobacteria a potential source for novel bioactives against biofilm-related infections.
RSC MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Syeda Warisul Fatima, Khalid Imtiyaz, Mohammad M. Alam Rizvi, Sunil K. Khare
Summary: The study examined the in vitro effects of microbial transglutaminase nanoflowers on breast cancer cells, revealing anti-cancer properties and significantly improved therapeutic efficacy on MCF-7 cells. The nanoflowers demonstrated cytotoxic effects and potential for cancer treatment.
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
