Article
Agricultural Engineering
Wriju Kargupta, Saroj Raj Kafle, Youngmoon Lee, Beom Soo Kim
Summary: The traditional production process of polyhydroxyalkanoate (PHA) from waste biomass requires a pretreatment step for sugar extraction and bacterial fermentation. This study explores a greener approach for PHA production from brown seaweed using Saccharophagus degradans. Cell retention cultures of S. degradans resulted in significantly higher PHA concentrations compared to batch cultures, indicating the potential for scalable and sustainable PHA production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Xinjuan Hu, Yulie E. Meneses, Jayne Stratton, Shuhao Huo
Summary: In this study, Saccharophagus degradans was used to degrade alginate and microalgae in AIM and produce PHB. The results showed that S. degradans effectively degraded and utilized polysaccharide of AIM and achieved high PHB production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Da Woon Jeong, Jeong Eun Hyeon, Myeong-Eun Lee, Young Jin Ko, Minhye Kim, Sung Ok Han
Summary: The study constructed alginate lyase complexes for efficient degradation of brown algal biomass, resulting in increased DEH and PHB production. The remaining biomass, such as fucoidan and laminaran, can also be utilized in the future for high value ingredients.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biotechnology & Applied Microbiology
Lanzeng Zhang, Xue Li, Xiyue Zhang, Yingjie Li, Lushan Wang
Summary: Recent research has focused on four aspects: alginate, alginate lyases, different alginate-degrading systems, and the application of alginate lyases and associated pathways, providing valuable insights for further application of alginate lyases and their associated pathways.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Nanna Rhein-Knudsen, Chengran Guan, Geir Mathiesen, Svein Jarle Horn
Summary: This study demonstrates that using culture supernatants directly as a source of alginate lyases is an efficient method for saccharification of seaweed biomass, improving the glucose concentration by 73% compared to using only cellulases.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Chemistry, Applied
Hylenne Bojorges, Amparo Lopez-Rubio, Maria Jose Fabra, Antonio Martinez-Abad
Summary: This study applied acid methanolysis and separation by anion exchange chromatography to determine the alginate content and M/G ratio. The results showed that methanolysis could be used to estimate the purity and M/G ratio of alginate-rich samples accurately in a single analysis.
CARBOHYDRATE POLYMERS
(2023)
Article
Biotechnology & Applied Microbiology
Katharina Nokling-Eide, Fangchang Tan, Shennan Wang, Qi Zhou, Mina Gravdahl, Anne-Mari Langeng, Vincent Bulone, Finn Lillelund Aachmann, Havard Sletta, Oystein Arlov
Summary: Cultivated brown algae are a potential source of carbohydrate polymers for biobased materials, but limited by a short harvest season and lack of cost-effective preservation methods. In this study, seaweed biomass were preserved with formic acid at low temperatures, resulting in increased yield of alginate and cellulose. The preservation had no effect on cellulose quality, while degraded the molecular weight of alginate. Acid preservation at low temperatures effectively stabilized seaweed biomass and preserved the quality of alginate and cellulose for biomaterials and other applications.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Review
Chemistry, Multidisciplinary
Zhiguo Zheng, Ali Dai, Yonggui Liu, Tingting Li
Summary: This article provides a brief introduction to the classification, source, and application of alginate lyase. Alginate lyase can catalytically degrade alginate and produce alginate oligosaccharide, which has a wide range of biological activities and is therefore of great importance in various fields.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuzuki Tanaka, Yoshihiro Murase, Toshiyuki Shibata, Reiji Tanaka, Tetsushi Mori, Hideo Miyake
Summary: In this study, recombinant enzymes were prepared and immobilized to efficiently convert alginate into DEH. The immobilized enzymes showed high activity even after repeated use, resulting in a relatively high DEH yield.
Article
Biochemistry & Molecular Biology
Fengchao Zhang, Zheng Fu, Luyao Tang, Zhelun Zhang, Feng Han, Wengong Yu
Summary: The novel exo-type alginate lyase VsAly7D isolated from brown algae showed the highest activity at 35 degrees C, with considerable activity at 20 and 30 degrees C, while exhibiting optimal activity under alkaline conditions. VsAly7D demonstrated efficient degradation of alginate, polyM, and polyG, making it a promising tool enzyme for bioethanol production from brown algae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Justyna M. Dobruchowska, Bryndis Bjornsdottir, Olafur H. Fridjonsson, Josef Altenbuchner, Hildegard Watzlawick, Gerrit J. Gerwig, Lubbert Dijkhuizen, Johannis P. Kamerling, Gudmundur O. Hreggvidsson
Summary: Alginate lyases are enzymes that catalyze the depolymerization of alginate, a linear polysaccharide. In this study, two alginate lyase genes were cloned and expressed from a marine thermophilic bacterium. The recombinant enzymes showed high thermostability and activity at elevated temperatures. The NMR analysis revealed that one of the enzymes is an endolytic lyase, while the other is an exolytic lyase.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Medicinal
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: Seaweeds, considered as third-generation renewable biomasses, have been gaining increasing attention for their comprehensive utilization. Researchers identified a new cold-active alginate lyase from Vibrio fortis and studied its biochemical characteristics for brown seaweed utilization. They also developed a bioconversion strategy for the utilization of brown seaweed.
Article
Microbiology
Bo Pilgaard, Marlene Vuillemin, Jesper Hoick, Casper Wilkens, Anne S. Meyer
Summary: Alginate is an anionic polysaccharide found in brown macroalgae, with enzymatic depolymerization mainly carried out by alginate lyases. A marine fungus, Paradendryphiella salina, was found to have four alginate lyases, including one PL8 and three PL7 enzymes. These enzymes showed different substrate specificities and activities in the depolymerization of alginate and polymannuronic acid.
Article
Polymer Science
Catalina Filote, Elhafnaoui Lanez, Valentin Popa, Touhami Lanez, Irina Volf
Summary: This study successfully separated and characterized fucoidan and alginate from brown macroalgae Fucus spiralis using an integrated processing method. The bioactivity and DNA binding abilities of the isolated polysaccharides were evaluated. The results showed that both fucoidan and alginate exhibited strong antioxidant activity and were able to interact with DNA.
Review
Biochemistry & Molecular Biology
Noora Barzkar, Ruilong Sheng, Muhammad Sohail, Saeid Tamadoni Jahromi, Olga Babich, Stanislav Sukhikh, Reza Nahavandi
Summary: The cell wall of brown algae contains a major constituent called alginate, which has various commercial applications. Alginate lyases play a significant role in marine carbon recycling and have potential applications in the food industry.
Article
Biochemistry & Molecular Biology
Hyeong Min Han, In Jung Kim, Eun Ju Yun, Jae Won Lee, Yoonho Cho, Yong-Su Jin, Kyoung Heon Kim
Summary: This study optimized the cultivation conditions of Escherichia coli for increased production of colanic acid. By statistically optimizing the culture media, glucose and tryptone were identified as the optimal carbon and nitrogen sources, leading to a significant enhancement in CA production. Through further optimization, a maximum CA production of 1910.0 mg/L was achieved, demonstrating the successful media optimization for large-scale production of CA.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Jungyeon Kim, Inho Jung, Yu Eun Cheong, Kyoung Heon Kim
Summary: Cofactors are crucial in the metabolism of living organisms, and understanding their concentration and extraction methods is essential. This study systematically compares and suggests optimal LC/MS methods for cofactor analysis and extraction methods from Saccharomyces cerevisiae.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Medicinal
Sora Yu, So Young Park, Dong Hyun Kim, Eun Ju Yun, Kyoung Heon Kim
Summary: 2-keto-3-deoxy sugar acids, including 2-keto-3-deoxy-l-galactonate (KDGal), can be biologically produced from plant-derived pectin and agarose. In this study, KDGal was enzymatically produced from agarose using the catabolic pathway of 3,6-anhydro-l-galactose (AHG), and purified by size-exclusion chromatography. This research demonstrates the potential of enzymatic production and purification of KDGal from agarose for high-value product synthesis.
Article
Chemistry, Multidisciplinary
In Jung Kim, Kyoung Heon Kim
Summary: This study reports a l-FucI enzyme with both thermophilic and halophilic properties, which exhibited optimal activity at 50-60 degrees C, pH 7, in the presence of 0.5-1 M NaCl and 1 mM Mn2+ as a cofactor. This unique enzyme has potential applications in the biotechnological production of l-fucose.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Ting Bu, Ming Zhang, Sun-Hee Lee, Yu Eun Cheong, Yukyung Park, Kyoung Heon Kim, Dongwon Kim, Sooah Kim
Summary: Skin has heterogenous identities on different body sites. The study used metabolomics analysis to investigate the metabolic differences between volar and non-volar skin in mice. It identified various metabolites associated with amino acid metabolism, sugar metabolism, and nucleotide metabolism. These findings provide valuable information for understanding the metabolic differences between volar and non-volar skin.
Article
Biochemistry & Molecular Biology
Jinkyung Lee, Suhyun Kim, Yoon Hwan Kim, Uiyeol Park, Junghee Lee, Ann C. McKee, Kyoung Heon Kim, Hoon Ryu, Jeongae Lee
Summary: This study utilized metabolomics to uncover the metabolic differences associated with cognitive dysfunction, amyloid-beta deposition, and neuroinflammation in the brains of patients with chronic traumatic encephalopathy (CTE). These findings provide new insights into the pathogenesis of CTE and suggest potential targets for diagnosis and treatment.
Article
Biotechnology & Applied Microbiology
Jungyeon Kim, Yu Eun Cheong, Sora Yu, Yong-Su Jin, Kyoung Heon Kim
Summary: The study constructed a yeast strain capable of metabolizing l-fucose in the gut, enhancing its metabolic activity. Through gene expression and metabolic modeling analysis, it was found that the yeast consumes l-fucose via a specific pathway. This study is significant for enhancing the metabolic activity of yeast and other probiotics in the gut.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Jungyeon Kim, Yong-Su Jin, Kyoung Heon Kim
Summary: L-fucose is an important metabolite in human-gut microbiome interactions. It is continuously produced by humans and delivered to the gut, where gut microorganisms metabolize it and produce short-chain fatty acids. Recent studies have shown that the carbon flux in L-fucose metabolism by gut microorganisms is different from other sugar metabolisms due to cofactor imbalance and low energy synthesis efficiency. The large amounts of short-chain fatty acids produced during microbial L-fucose metabolism are used by epithelial cells to recover the energy used up during L-fucose synthesis. This review provides an overview of microbial L-fucose metabolism and discusses the potential use of genetically engineered probiotics for disease treatment and prevention by modulating fucose metabolism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Sun-Hee Lee, Eun Ju Yun, Na Ree Han, Inho Jung, Jeffrey G. Pelton, Jae-Eun Lee, Nam Joo Kang, Yong-Su Jin, Kyoung Heon Kim
Summary: This study synthesized ethyl-agarobioside (ethyl-AB) through acid-catalyzed alcoholysis of agarose, which exhibited higher thermal and pH stability than AB, with similar skin-moisturizing activity. Ethyl-AB is a novel cosmetic ingredient with potential due to its high chemical stability.
Article
Biotechnology & Applied Microbiology
Eun Ju Yun, Sora Yu, Dong Hyun Kim, Na Jung Park, Jing-Jing Liu, Yong-Su Jin, Kyoung Heon Kim
Summary: A new metabolic pathway of 3,6-anhydro-l-galactose (l-AHG) in red macroalgae was discovered, and the enantiomeric nature of 2-keto-3-deoxy-galactonate (KDGal), a product of l-AHG catabolism, was identified. The study also demonstrated the utilization of l-KDGal by Escherichia coli. These findings have implications in determining the absolute configuration of compounds and constructing engineered microbial platforms for utilizing l-AHG or l-KDGal as substrates.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Young Seo Jang, Jungwoo Yang, Jae Kyun Kim, Tae In Kim, Yong-Cheol Park, In Jung Kim, Kyoung Heon Kim
Summary: In this study, isobutanol-tolerant mutants were isolated from two E. coli parental strains, E. coli BL21(DE3) and MG1655(DE3), through adaptive laboratory evolution (ALE) under high isobutanol concentrations. Subsequently, 16 putative genes responsible for isobutanol tolerance were identified by transcriptomic analysis. Overexpression of four genes (fadB, dppC, acs, and csiD) in E. coli conferred isobutanol tolerance and improved isobutanol titers. These findings are important for understanding microbial isobutanol tolerance and facilitating industrial isobutanol production.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Nutrition & Dietetics
Sun-Hee Lee, Tae-Joong Lim, Eun Ju Yun, Kyoung Heon Kim, Sanghyun Lim
Summary: This study investigated the effects of a combination of soybean germ extract and a probiotic on menopausal conditions and found that they can improve body weight, uterine and bone health, lipid profile, liver function, vascular markers, and neurotransmitter levels.
Article
Microbiology
Jungyeon Kim, Christine Atkinson, Michael J. Miller, Kyoung Heon Kim, Yong-Su Jin
Summary: Our study identified changes in the microbiome by administering wild-type S. boulardii in mice based on long-read sequencing and demonstrated the ability of engineered S. boulardii to secrete a recombinant protein that alters the microbiome. These findings provide valuable insights for the development of therapeutics using engineered S. boulardii that can modify the gut microbiome and host physiology.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Ye-Gi Lee, Hae-Yong Jo, Do-Haeng Lee, Jong-Won Yoon, Young-Ha Song, Dae-Hyuk Kweon, Kyoung Heon Kim, Yong-Cheol Park, Jin-Ho Seo
Summary: 2'-Fucosyllactose (2'-FL), a human milk oligosaccharide, was successfully synthesized using engineered Corynebacterium glutamicum. The optimized fed-batch cultivation strategy significantly increased the production and productivity of 2'-FL.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Endocrinology & Metabolism
Sooah Kim, Jiwon Hwang, Jungyeon Kim, Sun-Hee Lee, Yu Eun Cheong, Seulkee Lee, Kyoung Heon Kim, Hoon-Suk Cha
Summary: This study assessed the feasibility of using metabolomics to differentiate the metabolite profiles of synovial fluid between rheumatoid arthritis (RA) and osteoarthritis (OA). Results showed clear differences in metabolomic changes between RA and OA, with 28 crucial metabolites identified to play a role in distinguishing the two conditions.
