Article
Biochemistry & Molecular Biology
Zekai Gou, Zhibo Peng, Shiyu Wang, Liuyang Chen, Zhicong Ma, Yifan Kang, Liang Sun, Rui Wang, Hong Xu, Yian Gu, Dafeng Sun, Peng Lei
Summary: In this study, SPG-producing strains of S. commune from different provinces in China were screened. A candidate strain (NTU-1) with a high SPG yield was chosen, and the fermentation conditions were optimized. The highest reported SPG concentration of 21.3 g/L was obtained in a 5-L bioreactor with a reduced fermentation cycle of five days.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Qi Meng, ShiChen Chuai, Lei Chen, Lingling Wang, Guolin Cai, Jinsheng Mao, Zhenghua Gu, Guiyang Shi, Zhongyang Ding
Summary: This study investigated the production of polysaccharides through submerged fermentation of S. commune with the addition of Tween 80 as a surfactant, which resulted in increased polysaccharide production and shortened production cycle. The addition of Tween 80 led to polysaccharides with similar monosaccharide composition but lower molecular weight, and also elevated the transcription levels of genes involved in polysaccharide synthesis pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Xiaotian Chen, Jianrong Wu, Ziyun Shen, Song Xu, Xiaobei Zhan
Summary: In this study, ScOG with branched chain structures was obtained through co-culture of Schizophyllum commune and Trichoderma harzianum, and its sulfated derivatives (SScOG) were also prepared. The results showed that both ScOG and S-ScOG were resistant to digestion and had strong antioxidative activity. In vitro fermentation experiments demonstrated that ScOG and its derivatives increased the abundance of beneficial bacteria and the production of short-chain fatty acids. Overall, ScOG has good prebiotic potential and sulfation modification can enhance its prebiotic effects.
Article
Horticulture
Shweta Singh, Chandramani Raj, Harvinder Kumar Singh, Ravi Kant Avasthe, Prashant Said, Arumugam Balusamy, Susheel Kumar Sharma, Sangay Chuzem Lepcha, Varsha Kerketta
Summary: The study investigated the morphological and anatomical features of the common split-gilled mushroom, along with its cultural characteristics and potential substrates for large scale production. The research confirmed the best substrate for growing the mushroom is paddy straw, achieving the highest fresh weight yield of 91.9 gm/bag.
SCIENTIA HORTICULTURAE
(2021)
Article
Multidisciplinary Sciences
Sophia Wirth, Katrin Krause, Maritta Kunert, Selina Broska, Christian Paetz, Wilhelm Boland, Erika Kothe
Summary: Wood inhabiting fungi release volatiles that play an important role in wood decomposition and have activity against other fungi and bacteria. The volatiles of Schizophyllum commune inhibit fungal growth and modify bacterial motility. The microbial community in the mycosphere of S. commune includes Actinobacteria, Proteobacteria, Pseudomonadaceae, Sphingomonadaceae, Erwiniaceae, Yersiniaceae and Mariprofundacea as the dominating families, as well as ascomycetes and basidiomycetes in the fungal community.
Article
Microbiology
Xuan Liu, Muhammad Zain ul Arifeen, Yarong Xue, Changhong Liu
Summary: This study identified and classified the laccase genes in S. commune, revealing differences in intron-exon structures, conserved motifs, and cis-acting elements. Analysis of resequencing data found significant differences in laccase genes between S. commune from different environments, possibly related to different environmental pressures and substrate inductions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yunan Ma, Mengshi Zhao, Fan Zhou, Xuan Liu, Changhong Liu
Summary: This study presents the first evidence of anaerobic exopolysaccharide (EPS) synthesis by the fungus Schizophyllum commune 20R-7-F01, isolated from coal-bearing sediments 2.0 km below the seafloor. The fungus exhibited significantly higher specific EPS production under anaerobic conditions compared to aerobic conditions. Transcriptomic analysis revealed differentially expressed genes associated with carbon metabolism pathways, highlighting the oxygen-dependent regulation of EPS synthesis and suggesting its importance for fungal adaptation to anaerobic environments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Te-Kai Sun, Wen-Chin Huang, Yu-Wen Sun, Jeng-Shyan Deng, Liang-Hsuan Chien, Ya-Ni Chou, Wen-Ping Jiang, Jaung-Geng Lin, Guan-Jhong Huang
Summary: The outbreak of COVID-19 caused by SARS-CoV-2 has had a significant global impact, and there are still uncertainties regarding the virus's impact on human health. However, research has found that Schizophyllum commune can reduce the expression of both ACE2 and TMPRSS2 proteins, potentially inhibiting SARS-CoV-2 infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Shima Dabaghi, Seyed Ahmad Ataei, Ali Taheri
Summary: This study identified and optimized the key process parameters for the synthesis of rhamnolipids using soybean meal in solid state fermentation. The optimized conditions resulted in a rhamnolipid production of 19.68 g/kg dry substrate, with low surface tension and good emulsification activity.
Article
Engineering, Environmental
Lea Traxler, Anne Wollenberg, Georg Steinhauser, Ihor Chyzhevskyi, Sergiy Dubchak, Sina Grossmann, Alix Guenther, Dharmendra Kumar Gupta, Karl-Heinz Iwannek, Serhii Kirieiev, Falk Lehmann, Wolfgang Schulz, Clemens Walther, Johannes Raff, Erika Kothe
Summary: Research conducted in the Chernobyl exclusion zone demonstrates that the basidiomycete fungus Schizophyllum commune can survive and spread rapidly in soil contaminated with radiation and heavy metals.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Lea Traxler, Jenny Shrestha, Martin Richter, Katrin Krause, Thorsten Schafer, Erika Kothe
Summary: Fungi living in heavy metals and radionuclides contaminated environments, such as the Chernobyl Exclusion Zone, have developed mechanisms to cope with these pollutants. The wood-rot fungus Schizophyllum commune was studied for its metal tolerance mechanisms and ability to transport metals through its hyphae. Adapted strains showed specific metal transport and a lack of changes in morphology, indicating metal stress avoidance mechanisms. Transporter genes were downregulated while genes involved in the secretory pathway were upregulated under metal stress.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Applied
Chuencheewit Thongsiri, Yoshie Nagai-Yoshioka, Ryota Yamasaki, Yoshiyuki Adachi, Michihiko Usui, Keisuke Nakashima, Tatsuji Nishihara, Wataru Ariyoshi
Summary: During this study, the biological effect of Schizophyllan, a beta-glucan from Schizophyllum commune, on IL-10 expression in murine macrophages was investigated. It was found that SPG enhances IL-10 expression by prolonging the nuclear translocation activity of the NF-kappa B alpha pathway induced by LPS, and by stimulating phosphorylation of MSK1 and CREB, thus promoting an anti-inflammatory response in murine macrophages.
CARBOHYDRATE POLYMERS
(2021)
Article
Food Science & Technology
Adwin Ong, Chi-Lik Ken Lee
Summary: This study investigated the effects of solid-state fermentation using okara and BSG as substrates with pure and mixed cultures of Aspergillus oryzae and Bacillus subtilis. The results showed that a mixed culture fermentation enhanced the nutritional profile of the substrates, particularly in terms of total phenolic content and total dietary fibre. However, challenges remain in accurately determining crude protein content, suggesting the need for more specific methods and sample preparation optimization in future research.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Jovana Miskovic, Maja Karaman, Milena Raseta, Nenad Krsmanovic, Sanja Berezni, Dragica Jakovljevic, Federica Piattoni, Alessandra Zambonelli, Maria Letizia Gargano, Giuseppe Venturella
Summary: In this study, different strains of Schizophyllum commune from Serbia and Italy were examined for their AChE inhibitory, antioxidative, and antibacterial activities. The polysaccharide (PSH) extracts showed the highest AChE inhibitory and antioxidative activities, making them promising candidates for Alzheimer's disease prevention. LC-MS/MS analysis identified p-hydroxybenzoic and gallic acids as the most abundant phenolic compounds in the extracts.
Article
Microbiology
Takahito Toyotome, Masahiko Takino, Masahiro Takaya, Maki Yahiro, Katsuhiko Kamei
Summary: The study revealed that volatile sulfur compounds produced by Schizophyllum commune could be potential targets for non-invasive diagnosis, with applications in identifying pathogens and infectious diseases.
Article
Agricultural Engineering
Ariane Fatima Murawski de Mello, Luciana Porto de Souza Vandenberghe, Clara Matte Borges Machado, Kim Kley Valladares-Diestra, Julio Cesar de Carvalho, Carlos Ricardo Soccol
Summary: The high costs of bioplastics' production may hinder their commercialization. Development of new processes with high yields and in biorefineries can enhance diffusion of these materials. This study evaluated the production of polyhydroxybutyrate (PHB) from corn starchy fraction hydrolysate and crude glycerol by Cupriavidus necator LPB 1421. The optimized process achieved a maximum accumulation of 62% PHB, with a productivity of 0.162 g.Lh-1. The potential application of corn as substrate for PHB production was confirmed, and the developed bioplastic process could be coupled to a bioethanol producing unit creating the opportunity of a sustainable and economic biorefinery.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Cristiano E. Rodrigues Reis, Nelson Libardi Junior, Heitor B. S. Bento, Ana Karine F. de Carvalho, Luciana Porto de Souza Vandenberghe, Carlos Ricardo Soccol, Tejraj M. Aminabhavi, Anuj K. Chandel
Summary: This review provides a comprehensive analysis of specific strategies, such as applying extraneous proteins and additives, enzyme recycling, and the effect of washing pre-treated biomass on the production of 2G sugar at large scales in lignocellulose biorefineries.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Plant Sciences
Amanda O. Rodrigues, Louise L. May L. De Mio, Carlos R. Soccol
Summary: Molecular studies have revealed Trichoderma's biocontrol mechanisms, making it an alternative to pesticides for disease control. Trichoderma-treated plants benefit from disease control and growth promotion through gene expression modulation. The commercialization and industrialization of Trichoderma technologies are increasing worldwide.
Article
Food Science & Technology
Alexander da Silva Vale, Gabriel Balla, Luiz Roberto Saldanha Rodrigues, Dao Pedro de Carvalho Neto, Carlos Ricardo Soccol, Gilberto Vinicius de Melo Pereira
Summary: This study investigated the microbial community structure and chemical changes in different layers of a static coffee beans fermentation tank over different time periods (24, 48, and 72 h). The results showed that extending the fermentation time resulted in increased production of key metabolites and diversification of sensory profiles of coffee beverages.
Article
Agricultural Engineering
Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Veronica Sayuri Nishida, Carlos Ricardo Soccol
Summary: This study evaluated the effects of imidazole pretreatment on the chemical composition and enzymatic hydrolysis efficiency of lignocellulosic biomasses. The results showed that the pretreatment increased the percentage of cellulose and improved enzymatic conversion, with a delignification level of up to 72%. Imidazole could be a potential catalyst for the valorisation of agro-industrial by-products.
BIORESOURCE TECHNOLOGY
(2023)
Editorial Material
Agricultural Engineering
Thallada Bhaskar, S. Venkata Mohan, Siming You, Sang-Hyoun Kim, Luciana Porto de Souza Vandenberghe
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Walter J. Martinez-Burgos, Luciana Porto de Souza Vandenberghe, Susan Grace Karp, Ariane Fatima Murawski de Mello, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Soybean hulls, a lignocellulosic residue from soybean processing, can be valorized as a carbon source for biolipid production. This study optimized the process for producing lipids from soybean hulls using Lipomyces starkeyi LPB 53 strain. The maximum cell biomass concentration reached was 26.5 g/L with 42.5% lipids, and a significant portion of xylose was consumed. The obtained oil was mainly composed of oleic, palmitic, palmitoleic, linoleic, and stearic fatty acids.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Sabrina Mores, Luciana Porto de Souza Vandenberghe, Walter Jose Martinez-Burgos, Cristine Rodrigues, Carlos Ricardo Soccol
Summary: This study evaluated the feasibility of using sugar-sweetened beverages (SSB) for citric acid (CA) production and its impact on the chemical oxygen demand (COD) of SSB. Five types of SSB were tested as carbon sources, with all showing suitability for CA production and effective reduction of COD. The use of SSB as a substrate provides a low-cost and readily available alternative to traditional feedstocks, while also reducing the environmental impact of the beverage industry through effective waste treatment and reuse.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Review
Multidisciplinary Sciences
Gustavo Amaro Bittencourt, Luciana Porto de Souza Vandenberghe, Walter Jose Martinez-Burgos, Kim Kley Valladares-Diestra, Ariane Fatima Murawski de Mello, Bruna Leal Maske, Satinder Kaur Brar, Sunita Varjani, Gilberto Vinicius de Melo Pereira, Carlos Ricardo Soccol
Summary: Emerging contaminants (ECs) have become a significant concern due to their widespread occurrence and inadequate removal efficiency in conventional wastewater treatment plants. Current research focuses on physical, chemical, and biological methods to minimize long-term risks to ecosystems. Enzyme-based processes, particularly oxidoreductases and hydrolases, offer higher efficiency and reduced toxic by-product generation. This review highlights recent advances in enzymatic processes for EC wastewater treatment, including immobilization techniques, genetic engineering, and the development of nanozymes. Future trends in enzyme immobilization techniques and the incorporation of enzymatic treatment in conventional wastewater treatment plants are discussed.
Review
Biotechnology & Applied Microbiology
Julio Cesar de Carvalho, Luciana Porto de Souza Vandenberghe, Eduardo Bittencourt Sydney, Susan Grace Karp, Antonio Irineudo Magalhaes Jr, Walter Jose Martinez-Burgos, Adriane Bianchi Pedroni Medeiros, Vanete Thomaz-Soccol, Sabrina Vieira, Luiz Alberto Junior Letti, Cristine Rodrigues, Adenise Lorenci Woiciechowski, Carlos Ricardo Soccol
Summary: Sugarcane ethanol production generates a large amount of vinasse, which can be used as a suitable substrate for anaerobic digestion to improve energy output. This review discusses the current technology for ethanol production from sugarcane and describes the state of the art in vinasse anaerobic digestion.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Alexander da Silva Vale, Barbara Cogo Venturim, Andre Ricardo Ferreira da Silva Rocha, Jose Guilherme Prado Martin, Bruna Leal Maske, Gabriel Balla, Juliano De Dea Lindner, Carlos Ricardo Soccol, Gilberto Vinicius de Melo Pereira
Summary: In recent years, the development of non-dairy probiotic beverages has been driven by the increasing number of people with milk protein allergies, lactose intolerance, and vegetarian and vegan diets. These functional foods have health benefits and combine plant properties with probiotic effects. However, the limited availability of suitable microbial strains with probiotic characteristics that also meet sensory requirements poses a challenge in formulating these beverages. This review provides an overview of traditional non-dairy fermented beverages worldwide and highlights the biotechnological potential of these products as a source of probiotic strains. It also discusses recent developments in using lactic acid bacteria, Bifidobacterium, and yeast species to create new probiotic beverages from fruits and cereals. Additionally, food safety issues are addressed.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Alexander da Silva Vale, Gilberto Vinicius de Melo Pereira, Ana Caroline de Oliveira, Dao Pedro de Carvalho Neto, Leonardo Wedderhoff Herrmann, Susan Grace Karp, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: The skin microbiome, consisting of bacteria, fungi, and viruses, plays a critical role in preventing the overgrowth of harmful microorganisms. Probiotic-derived metabolites, known as postbiotics, have emerged as promising bioactive compounds for skin health. Postbiotics, produced by lactic acid bacteria and yeast, possess antimicrobial, immunomodulatory, antioxidant, and anti-inflammatory activities. They also offer advantages in terms of stability and safety compared to probiotics. This article reviews the large-scale production of postbiotics and their applications in cosmetic formulations, as well as discussing their efficacy in treating various skin conditions. Technological advancements in the field of postbiotics are also highlighted through a survey of patents.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
Renato L. Binati, Nicola Ferremi Leali, Michele Avesani, Elisa Salvetti, Giovanna E. Felis, Francesca Monti, Sandra Torriani
Summary: This study characterized and compared the cell wall composition of different industrial and native wine yeast strains using various microscopy techniques and ATR-FTIR microspectroscopy. The findings revealed significant differences among the strains in terms of cell wall thickness, cell diameter, and relative concentration of beta-glucans and mannans. ATR-FTIR microspectroscopy proved to be a valuable tool for identifying polysaccharide-rich yeast strains, which can be useful for selecting starter cultures in the wine and food industry.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Review
Food Science & Technology
Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Sabrina Vieira, Luis Daniel Goyzueta-Mamani, Patricia Beatriz Gruening de Mattos, Maria Clara Manzoki, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Concerns about a good-quality diet have risen in recent years, leading to an increased interest in food supplements like prebiotics that offer great nutritional and health benefits. Xylooligosaccharides (XOs) show high potential among various prebiotics for preventing systemic disorders. While XOs can be found in natural sources, their production is limited. Lignocellulosic biomasses have been identified as a promising source for XOs production, using agro-industrial by-products. However, physicochemical pretreatments are required to obtain XOs, and different methods are discussed to increase their production and limit the production of toxic compounds.
Article
Biotechnology & Applied Microbiology
Priscilla Zwiercheczewski de Oliveira, Luciana Porto de Souza Vandenberghe, Carlos Ricardo Soccol
Summary: This study presents the production of lactic acid from sugarcane juice and explores the use of ion-exchange resins for its purification. The fermentation kinetics showed the highest lactic acid production with a productivity of 1.18 g LA/L center dot h. The combined use of Amberlite IR120 and IRA-67 resins in a stirred tank resulted in a final lactic acid concentration of 189.11 g/L with 95% mass recovery. This simplified approach demonstrates the viability of using ion-exchange resins in a controlled environment and for scale-up processes.
FERMENTATION-BASEL
(2023)