期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 407, 期 17, 页码 5189-5198出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8585-6
关键词
Ceramides; Sphingomyelins; Sphingosine-1-phosphate; Sphinganine-1-phosphate; LC-MS/MS; Human plasma
资金
- National Institutes of Health [DK073955, DA012413]
We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid-liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified.
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