期刊
BIOPOLYMERS
卷 95, 期 5, 页码 290-297出版社
WILEY
DOI: 10.1002/bip.21574
关键词
super-resolution fluorescence microscopy; DNA intercalators; photophysics; single-molecule methods
资金
- EPSRC [EP/F042248/1]
- The Royal Society [UF090182]
- EPSRC [EP/F042248/1] Funding Source: UKRI
- Royal Society [UF090182] Funding Source: Royal Society
- Engineering and Physical Sciences Research Council [EP/F042248/1] Funding Source: researchfish
With the expansion of super-resolution fluorescence microscopy methods, it is now possible to access the organization of cells and materials at the nanoscale by optical means. This review discusses recent progress in super-resolution imaging of isolated and cell DNA using single-molecule localization methods. A high labeling density of photoswitchable fluorophores is crucial for these techniques, which can be provided by sequence independent DNA stains in which photoblinking reactions can be induced. In particular, unsymmetrical cyanine intercalating dyes in combination with special buffers can be used to image isolated DNA with a spatial resolution of 30-40 nm. For super-resolution imaging of chromatin, cell permeant cyanine dyes that bind the minor groove of DNA have the potential to become a useful alternative to the labeling of histones and other DNA-associated proteins. Other recent developments that are interesting in this context such as high density labeling methods or new DNA probes with photoswitching functionalities are also surveyed. Progress in labeling, optics, and single-molecule localization algorithms is being rapid, and it is likely to provide real insight into DNA structuring in cells and materials. (C) 2010 Wiley Periodicals, Inc. Biopolymers 95: 290-297, 2011.
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