Article
Biochemistry & Molecular Biology
Alexandra Primikyri, Georgios Papamokos, Themistoklis Venianakis, Marianna Sakka, Vassiliki G. Kontogianni, Ioannis P. Gerothanassis
Summary: This study investigates the structural basis of the interaction between artemisinin and human and bovine serum albumin using a combined strategy of experiments and calculations. The results suggest that artemisinin competes for binding sites with other drugs and interacts with bovine serum albumin in the presence of A. annua extract.
Review
Chemistry, Multidisciplinary
Ioannis P. Gerothanassis
Summary: Natural product-observed NMR methods provide significant advantages for in-tube NMR monitoring of enzyme biotransformations and investigating protein-natural product interactions in living cells. The use of a NMR bioreactor allows for investigating natural products as enzyme substrates, monitoring multiple biotransformation products, and studying interactions with protein targets. This method, along with computational techniques, shows promise in protein-natural product recognition research. A rating of: 6/10.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Cristina Di Carluccio, Maria Concetta Forgione, Silvia Martini, Francesco Berti, Antonio Molinaro, Roberta Marchetti, Alba Silipo
Summary: Molecular recognition is crucial in biology, and the combination of NMR techniques with computational methods allows for the accurate determination of protein-ligand complex 3D models, providing key structural insights into ligand-receptor interactions.
CARBOHYDRATE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Rossella Di Guida, Angela Casillo, Juan M. Tomas, Susana Merino, Maria Michela Corsaro
Summary: Aeromonas species are pathogenic bacteria found in various environments and foods. In this study, the structure of the O-chain repeating unit of the LPS from Aeromonas bivalvium strain 868 E-T was determined, providing insights into the pathogenicity of this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Shanmugam Vanithamani, Charles Solomon Akino Mercy, Murugesan Kanagavel, Krishnamoorthi Sumaiya, Palanisamy Bothammal, Perumal Saranya, Muthu Prasad, Karuppiah Ponmurugan, Gangatharan Muralitharan, Naif Abdullah Al-Dhabi, Ashutosh Verma, Paluru Vijayachari, Kalimuthusamy Natarajaseenivasan
Summary: This study analyzed and compared the genes involved in Leptospira lipopolysaccharide biosynthesis and performed chemical composition analysis of lipopolysaccharides from different serovars. The results showed significantly higher endotoxicity levels in pathogenic serovars compared to nonpathogenic ones. Specific components identified in the different lipopolysaccharides may be significant factors contributing to pathogen-associated predisposition.
MICROBIAL PATHOGENESIS
(2021)
Review
Chemistry, Physical
Pedro Lameiras, Jean-Marc Nuzillard
Summary: The nuclear Overhauser effect (NOE) is a result of cross-relaxation between nuclear spins mediated by dipolar coupling, becoming increasingly important in determining through-space atom proximity relationships within molecules. The efficiency of NOE depends on the molecular rotational correlation time, and increasing solution viscosity can reveal otherwise unobservable NOEs.
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
(2021)
Article
Biochemical Research Methods
Kurt Wuthrich
Summary: The development of protein structure determination by NMR in solution from 1968 to 1992 was driven by identifying NMR-accessible parameters and developing new experimental methods. Breakthroughs during the years 1976-1984 include the development of NOE experiments, solving the phase problem, generating distance geometry algorithms, and introducing two-dimensional NMR for improved spectral resolution.
JOURNAL OF MAGNETIC RESONANCE
(2021)
Article
Chemistry, Medicinal
Gopal Pandit, Nabarupa Chowdhury, Sk. Abdul Mohid, Anil P. Bidkar, Anirban Bhunia, Sunanda Chatterjee
Summary: This study reports the efficacy and toxicity of three de novo designed cationic antimicrobial peptides (AMPs) against a range of pathogens, highlighting the importance of optimal hydrophobic interactions in generating highly potent non-cytotoxic AMPs. Among the AMPs tested, VV-14 was found to be non-cytotoxic and highly potent under physiological salt concentrations, able to form amphipathic helical conformations in the presence of microbial membrane mimics. The activity of these AMPs is related to their ability to interact with cell membrane lipids through hydrophobic interactions, determining their antimicrobial potency and cytotoxicity.
Article
Biochemistry & Molecular Biology
Dipita Bhattacharyya, Anirban Bhunia
Summary: Recent studies suggest that Parkinson's disease may begin in the gastrointestinal tract decades before symptoms manifest in the central nervous system. The gut-brain axis, involving the unique microbial composition of the enteric nervous system, plays a critical role in neurodegenerative diseases. Compromised gut health can lead to the translocation of gut microbiota and their metabolites into the body, affecting homeostasis.
CHEMISTRY AND PHYSICS OF LIPIDS
(2021)
Editorial Material
Chemistry, Multidisciplinary
Haribabu Arthanari, Kandala V. R. Chary, Thomas Szyperski, Krishna V. Ramanathan, Ramakrishna V. Hosur, Nagarajarao Suryaprakash, Srinivasarao Raghothama, Naranamangalam R. Jagannathan, Raymond S. Norton, Erode N. Prabhakaran, Paturu Kondaish, Sulakshana P. Mukherjee, Satish Kumar Adiga, RamaKrishna Vadrevu, Anirban Bhunia, David Joseph
MAGNETIC RESONANCE IN CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Li Jin, Chunhong Liu, Ning Zhang, Ruiyan Zhang, Mingdi Yan, Anirban Bhunia, Qinxiu Zhang, Min Liu, Jun Han, Hans-Christian Siebert
Summary: Captopril inhibits human lysozyme fibrillation by reducing the beta-sheet structure and particle size of amyloid fibrils, and forming hydrogen bonds with specific amino acid residues of lysozyme. This inhibition mechanism helps prevent the formation of amyloid fibrils and protects cells from amyloid cytotoxicity.
Article
Biochemistry & Molecular Biology
Shruti Mukherjee, Amaravadhi Harikishore, Anirban Bhunia
Summary: Envelope (E) proteins play crucial roles in SARS-CoV-1 and NCOVID19 viruses, assembling into homopentamers and affecting viral replication through glycosylation motif and hydrophobic helical bundle regions. This study developed a SARS-2 E protein model based on the structure of SARS-CoV-1 E protein, focusing on potential drug targets for inhibiting viral replication.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Wen Gao, Li Jin, Chunhong Liu, Ning Zhang, Ruiyan Zhang, Zuzana Bednarikova, Zuzana Gazova, Anirban Bhunia, Hans-Christian Siebert, Huijun Dong
Summary: In this study, the inhibitory effects of Sennoside A and Sennoside C on amyloid fibrillation were evaluated using human lysozyme as a model. Both compounds were found to inhibit the fibrillation of lysozyme in a dose-dependent manner, with interactions mainly through hydrogen bonds, vander-Waals forces, and hydrophobic interactions. Transmission electron microscopy and atomic force microscopy confirmed the reduction in fibrillation, supporting the conclusion that both Sennoside A and Sennoside C can significantly inhibit amyloid fibrillation of lysozyme.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biology
Ritobrita Chakraborty, Sandip Dey, Pallabi Sil, Simanta Sarani Paul, Dipita Bhattacharyya, Anirban Bhunia, Jayati Sengupta, Krishnananda Chattopadhyay
Summary: The study presents a novel mechanism of stabilizing alpha-synuclein oligomers by heme, which targets the His50 residue and alters the protein conformation to form non-toxic, non-fibrillating oligomers. This findings provide important insights into the structure of transient oligomers and offer a potential therapeutic strategy for preventing amyloid fibril formation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ipsita Chakraborty, Rajiv K. Kar, Dibakar Sarkar, Sourav Kumar, Nakul C. Maiti, Atin Kumar Mandal, Anirban Bhunia
Summary: The dynamics of solvents play a significant role in the fibrillation of amyloidogenic systems, and can be effectively studied through NMR methods. Validation with MD simulations has shown the pivotal role of solvent environment in linking aggregation properties of amyloid systems. Time-dependent modulation of solvent dynamics probed with NMR can provide insights into amyloidogenesis and toxicity profiles.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemical Research Methods
Humaira Ilyas, Mariena J. A. van der Plas, Monica Agnoletti, Sourav Kumar, Atin Kumar Mandal, Hanudatta S. Atreya, Anirban Bhunia, Martin Malmsten
Summary: The study investigated how PEGylation affects the interaction of host defense peptides with bacterial lipopolysaccharide and the suppression of LPS-induced cell activation. The results showed that PEGylation did not compromise the anti-inflammatory effects of the peptides and even improved the suppression of LPS-induced cell activation.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Kamal D. Patel, Sk Abdul Mohid, Arkajyoti Dutta, Shalini Arichthota, Anirban Bhunia, Devyani Haldar, Vijayalekshmi Sarojini
Summary: This study developed a novel substrate-based sirtuin inhibitor that successfully penetrated bacterial cell membranes and inhibited bacterial transcription activity, showing excellent antibacterial potency against both Gram-negative and Gram-positive pathogens. These peptides also demonstrated the ability to inhibit biofilm formation, disrupt preformed biofilms, and showed selectivity towards bacterial membranes, suggesting potential therapeutic applications for antimicrobial treatment.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Cell Biology
Akanksha Gandhi, Rupesh Kariyat, Amaravadhi Harikishore, Marzieh Ayati, Anirban Bhunia, Nirakar Sahoo
Summary: Plants and insect herbivores engage in a relentless battle, with plants evolving strategies to detect and defend against herbivores through physical, structural, and chemical barriers. Plants perceive herbivory through mechanical and chemical means, with ion channels acting as first responders in defense signaling pathways. Recent studies have focused on early plant defense signaling in response to herbivory, highlighting the crucial role of ion channels.
Article
Biochemistry & Molecular Biology
Kanti Kusum Yadav, Monalisha Ojha, Ranit Pariary, Manoranjan Arakha, Anirban Bhunia, Suman Jha
Summary: The study explores the effects of surface potential and functional groups on the fibrillation of bovine insulin using zinc oxide nanoparticles. It is found that zinc oxide nanoparticles with positive surface potential enhance insulin fibrillation, but the addition of amino acids as surface functional groups mitigates this effect. The change in interfacial functional groups also reverses the interface-mediated destabilization of insulin conformation. The interaction between the amino acid functional groups and key amyloidogenic sequences of insulin is believed to stabilize the conformation and reduce fibrillation and cytotoxicity.
Review
Biochemistry & Molecular Biology
Surajit Bhattacharjya, Sk Abdul Mohid, Anirban Bhunia
Summary: Antimicrobial peptides (AMPs) are effective therapeutic modalities against drug-resistant Gram-negative bacteria. This review article discusses the 3-D structures and mode of actions of several potent AMPs, as well as studies on human peptide LL37 and de novo-designed peptides. The challenges in clinical trials of AMPs are also summarized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hans-Christian Siebert, Thomas Eckert, Anirban Bhunia, Nele Klatte, Marzieh Mohri, Simone Siebert, Anna Kozarova, John W. Hudson, Ruiyan Zhang, Ning Zhang, Lan Li, Konstantinos Gousias, Dimitrios Kanakis, Mingdi Yan, Jesus Jimenez-Barbero, Tibor Kozar, Nikolay E. Nifantiev, Christian Vollmer, Timo Brandenburger, Detlef Kindgen-Milles, Thomas Haak, Athanasios K. Petridis
Summary: The global outbreak of SARS-CoV-2/COVID-19 has provided an opportunity and challenge to accumulate a large amount of biomedical data and test new concepts and strategies to combat the pandemic. This study correlated clinical biomedical data with structural biology and biophysical data to evaluate new diagnostic and therapeutic approaches against SARS-CoV-2. The methods used were effective in identifying potential peptide sequences and carbohydrate moieties to protect the blood-brain barrier and develop new therapeutic approaches against long-COVID symptoms.
Article
Chemistry, Multidisciplinary
Ranit Pariary, Sandip Dolui, Gourav Shome, Sk Abdul Mohid, Achintya Saha, Bhisma N. Ratha, Amaravadhi Harikishore, Kuladip Jana, Atin K. Mandal, Anirban Bhunia, Nakul C. Maiti
Summary: Our studies demonstrate that Coomassie Brilliant Blue G-250 shows promise as a chemical chaperone for stabilising the alpha-helical native human insulin conformers, preventing their aggregation and increasing insulin secretion. This multipolar effect, along with its non-toxic nature, could be valuable for the development of highly bioactive, targeted, and biostable therapeutic insulin.
CHEMICAL COMMUNICATIONS
(2023)