期刊
BIOPHYSICAL JOURNAL
卷 106, 期 10, 页码 2096-2104出版社
CELL PRESS
DOI: 10.1016/j.bpj.2014.03.044
关键词
-
类别
资金
- Inserm
- CNRS
- Fondation pour la Recherche Medicale (FRM) [FRM-DEQ-20090515412]
- Agence Nationale de la Recherche (ANR) [ANR-10-BLAN-1214, ANR-10-INBS-04, ANR-11-LABX-0054, ANR-11-IDEX-0001-02, ANR-12-BSV3-0001]
- European Research Council [ERC-2010-AdG 20100317]
- Ligue Nationale Contre le Cancer
- National Institutes of Health (NIH) Office of Research Infrastructure Programs [P40 OD010440]
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV3-0001, ANR-10-BLAN-1214] Funding Source: Agence Nationale de la Recherche (ANR)
To investigate the early stages of cell-cell interactions occurring between living biological samples, imaging methods with appropriate spatiotemporal resolution are required. Among the techniques currently available, those based on optical trapping are promising. Methods to image trapped objects, however, in general suffer from a lack of three-dimensional resolution, due to technical constraints. Here, we have developed an original setup comprising two independent modules: holographic optical tweezers, which offer a versatile and precise way to move multiple objects simultaneously but independently, and a confocal microscope that provides fast three-dimensional image acquisition. The optical decoupling of these two modules through the same objective gives users the possibility to easily investigate very early steps in biological interactions. We illustrate the potential of this setup with an analysis of infection by the fungus Drechmeria coniospora of different developmental stages of Caenorhabditis elegans. This has allowed us to identify specific areas on the nematode's surface where fungal spores adhere preferentially. We also quantified this adhesion process for different mutant nematode strains, and thereby derive insights into the host factors that mediate fungal spore adhesion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据