Article
Immunology
Lina S. Silva-Bermudez, Tatyana N. Sevastyanova, Christina Schmuttermaier, Carolina De La Torre, Leonie Schumacher, Harald Klueter, Julia Kzhyshkowska
Summary: Titanium nanoparticles (TiNPs) have a dual effect on the GDF-15/stabilin-1 interaction in macrophage system, by increasing the production of GDF-15 and suppressing stabilin-1-mediated clearance function. This can lead to unbalanced pro-fibrotic reactions and implant complications.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Ying Zhang, Jing Zhu, Hao Xu, Qin Yi, Liang Yan, Liang Ye, Xinyuan Zhang, Min Xie, Bin Tan
Summary: Using real-time confocal microscopy and structured illumination microscopy, we observed the time-dependent internalization of fluorescently labeled S100B protein (S100B-Alexa488) into MSCs and its transport through endolysosomal pathways. Inhibitors and RNA interference approaches further demonstrated that clathrin and lipid rafts play important roles in the internalization of S100B-Alexa488, providing potential interventions for the safe application of MSCs in glioma therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Catherine A. Tindall, Kevin Moehlis, Inka Rapoehn, Sebastian Dommel, Veronika Riedl, Michael Schneekoenig, Corinna Hoefling, Steffen Rossner, Jan Stichel, Annette G. Beck-Sickinger, Juliane Weiner, John T. Heiker
Summary: This study reveals the uptake and degradation mechanism of Vaspin in adipocytes, and proposes the Vaspin-LRP1 axis as an important mediator of Vaspin effects not only in adipose tissue but also in other LRP1-expressing cells.
Article
Chemistry, Physical
Laura I. FitzGerald, Angus P. R. Johnston
Summary: The review discusses current approaches for measuring the cellular uptake of nanoparticles and proteins, with a focus on recent advances in sensor technology. It highlights the requirements for quantifying internalization and potential pitfalls in data analysis. The importance of understanding recycling of internalized material back to the cell surface and methods for quantifying this process are also explored.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mai Itagaki, Yoshinori Nasu, Chiaki Sugiyama, Ikuhiko Nakase, Noriyasu Kamei
Summary: The study developed a method to evaluate cellular endocytosis through three main pathways. It was found that common endocytosis inhibitors had no specific inhibitory effect or were cytotoxic. The researchers successfully established alternative methods using RNA interference and rottlerin to specifically analyze clathrin- and caveolae-mediated endocytosis and macropinocytosis, respectively. The proposed methods were validated using biological molecules and carriers.
Article
Chemistry, Physical
Abash Sharma, Yu Zhu, Eric J. Spangler, Jan-Michael Y. Carrillo, Mohamed Laradji
Summary: This study investigates the adhesion and endocytosis modes of two spherocylindrical nanoparticles (SCNPs) on planar and tensionless lipid membranes through systematic molecular dynamics simulations. The results show that under weak adhesion energy, the SCNPs adhere to the membrane individually in a parallel mode. As the adhesion energy increases, the SCNPs form wedged dimers with an obtuse angle between their major axes. The final adhesion state and endocytosis mode of the SCNPs are strongly influenced by their initial distance and the value of adhesion energy.
Article
Multidisciplinary Sciences
Maria Romanova-Michaelides, Zena Hadjivasiliou, Daniel Aguilar-Hidalgo, Dimitris Basagiannis, Carole Seum, Marine Dubois, Frank Juelicher, Marcos Gonzalez-Gaitan
Summary: Research has found that scaling of the Dpp gradient in the Drosophila wing disc is achieved by increasing the contribution of internalized Dpp molecules to Dpp transport, regulated by the extracellular factor Pentagone. This evolutionary mechanism may act on endocytic trafficking to regulate the range and scaling of the gradient, allowing adaptation of shape and pattern to different sizes of organs in different species.
Article
Multidisciplinary Sciences
Hayley Muendlein, Wilson M. Connolly, Zoie Magri, Irina Smirnova, Vladimir Ilyukha, Avishekh Gautam, Alexei Degterev, Alexander Poltorak
Summary: In this study, the TRIF-dependent complex plays a crucial role in cell death and inflammation response, particularly in the context of bacterial infection. The constitutive binding between ZBP1 and RIPK1 is essential for TRIFosome interactions, caspase-8-mediated cell death, and inflammasome activation. This research provides an alternative model of pro-death complex formation reliant on TRIF signaling, demonstrating a non-canonical cell death pathway controlled by a complex of FADD, RIPK1, caspase-8, and ZBP1.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Akkaranunt Supakijsilp, Jing He, Xubo Lin, Jian Ye
Summary: This study investigates the interaction between nanoparticles and the pulmonary surfactant monolayer, focusing on the effects of nanoparticle elasticity on translocation across the monolayer. Molecular dynamics simulations reveal that increasing stiffness enhances the penetrability of nanoparticles, while soft nanoparticles remain trapped within the monolayer. Active cellular uptake pathways exhibit longer penetration times, greater penetration distances, and more contacts compared to passive pathways, due to ligand-receptor interactions.
Article
Medicine, Research & Experimental
Ryan C. Gimple, Guoxin Zhang, Shuai Wang, Tengfei Huang, Jina Lee, Suchet Taori, Deguan Lv, Deobrat Dixit, Matthew E. Halbert, Andrew R. Morton, Reilly L. Kidwell, Zhen Dong, Briana C. Prager, Leo J. Y. Kim, Zhixin Qiu, Linjie Zhao, Qi Xie, Qiulian Wu, Sameer Agnihotri, Jeremy N. Rich
Summary: In this study, the researchers found that SNX10 was selectively expressed in glioblastoma stem cells (GSCs) compared with non-neoplastic neural stem cells (NSCs). SNX10 was essential for GSC survival and promoted GSC proliferation and stem cell signaling pathways through posttranscriptional regulation of PDGFR tyrosine kinase. Targeting SNX10 expression decreased GSC viability and self-renewal capacity. High expression of SNX10 correlated with poor prognosis in glioblastoma patients.
Article
Virology
Zhaoyang Ji, Hui Dong, Ruixue Jiao, Xiaoyuan Zhu, Hongyan Shi, Jianfei Chen, Da Shi, Jianbo Liu, Zhaoyang Jing, Jialin Zhang, Xiaobo Wang, Dandan Ye, Jiyu Zhang, Xin Zhang, Li Feng
Summary: The coronavirus membrane protein plays a central role in the viral life cycle, and this study reveals a new role for the M protein in the initial stages of TGEV replication. The interaction between HSC70 and M protein mediates the internalization of TGEV through clathrin-mediated endocytosis. These findings provide new insights into the life cycle of coronaviruses and may aid in the development of therapeutic agents for porcine diarrhea.
JOURNAL OF VIROLOGY
(2023)
Article
Microbiology
Xidian Tang, Yanfei Xie, Guanhua Li, Zhannur Niyazbekova, Shaofei Li, Jianjun Chang, Dekun Chen, Wentao Ma
Summary: The entry of ORFV into target cells is mediated by clathrin-mediated endocytosis and macropinocytosis, while caveolae-dependent endocytosis has minor effects. Additionally, ORFV entry into cells requires an acidic environment and the involvement of dynamin.
VETERINARY MICROBIOLOGY
(2023)
Review
Crystallography
Xiaohuan Wang, Long Li, Fan Song
Summary: NPs are widely used as drug carriers in drug delivery due to their unique physical and structural properties. The interplay of NP properties, such as size, shape, and surface functionality, plays a crucial role in determining the final drug delivery efficacy through receptor-mediated endocytosis. Understanding these interactions can greatly enhance the design of NP platforms for drug delivery.
Article
Multidisciplinary Sciences
Masataka Michigami, Kentaro Takahashi, Haruna Yamashita, Zhengmao Ye, Ikuhiko Nakase, Ikuo Fujii
Summary: The generation of ligand-targeting peptidedrug conjugates (PDCs) provides a new approach for targeted intracellular drug delivery utilizing receptor-mediated endocytosis. The designed helix-loop-helix (HLH) peptide acts as the drug carrier, forming a ternary complex with VEGF and its receptor, leading to internalization into cells via VEGF receptor-mediated endocytosis. These HLH peptides and their PDCs show great potential as a new modality for targeted molecular therapy.
Article
Pharmacology & Pharmacy
Qiang Wu, Song Chen, Hanchen Zhu, Na Xu, Qianhua Yang, Wenbing Yao, Xiangdong Gao
Summary: This study focused on the characteristics and optimization of GLP-1 receptor endocytosis, using various methods to analyze the effects and screen potential agonists for treatment of type 2 diabetes mellitus. The results showed that endocytosis of GLP-1 receptor-mediated elimination was clathrin-dependent, and agonists biased towards the G protein pathway had less endocytosis. The identified analogue M4 showed prolonged hypoglycaemic activities and a long half-life, making it a potential lead compound for type 2 diabetes mellitus treatment.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
