Article
Chemistry, Physical
Cecilia Zaza, German Chiarelli, Ludovit P. Zweifel, Mauricio Pilo-Pais, Evangelos Sisamakis, Fabio Barachati, Fernando D. Stefani, Guillermo P. Acuna
Summary: Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited volume, which limits the spatial information, accuracy, and dynamic range of the observed signals. Here, an approach to obtain super-resolved FRET imaging based on single-molecule localization microscopy using an early prototype of a commercial time-resolved confocal microscope is demonstrated. DNA Points Accumulation for Imaging in Nanoscale Topography with fluorogenic probes provides a suitable combination of background reduction and binding kinetics compatible with the scanning speed of usual confocal microscopes. A single laser is used to excite the donor, a broad detection band is employed to retrieve both donor and acceptor emission, and FRET events are detected from lifetime information.
Article
Multidisciplinary Sciences
Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J. R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini
Summary: The feasibility of super-resolution microscopy for live and thick sample imaging is limited. We propose a method that combines ISM and STED techniques to achieve high-resolution imaging without introducing photodamage and background degradation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Eileen S. Hwang, Denise J. Morgan, Jieliyue Sun, M. Elizabeth Hartnett, Kimani C. Toussaint, Brittany Coats
Summary: Confocal reflectance microscopy was used to evaluate the collagen structure of the vitreous. The study successfully imaged the collagen network using intrinsic reflectance and optical sectioning, revealing a higher density of fibers in the anterior region near the vitreous base.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Cardiac & Cardiovascular Systems
Aditya K. Kaza, Robert Hitchcock, Abhijit Mondal, Stephanie Tuttle, Brian Cottle, Frank B. Sachse
Summary: This study introduces a new technique for identifying regions of conduction tissue in the heart using fiberoptic confocal microscopy. Evaluation of the technique on patients with congenital heart defects revealed substantial variability in the location of the conduction system. The clockface schematics were used to create surgical repair plans based on this variability.
ANNALS OF THORACIC SURGERY
(2022)
Article
Optics
Tristan C. Paul, Guy Hagen
Summary: A new technique called Spinning Disk Structured Illumination Microscopy (SD-SIM) was investigated, which combines Spinning Disk microscopy with Structured Illumination Microscopy to achieve better background removal and detect cellular structures that were previously undetectable with either method alone.
Article
Urology & Nephrology
Jesus Z. Villarreal, Javiera Perez-Anker, Susana Puig, Marc Xipell, Gerard Espinosa, Esther Barnadas, Ana B. Larque, J. Malvehy, Ricard Cervera, Arturo Pereira, Antonio Martinez-Pozo, Luis F. Quintana, Adriana Garcia-Herrera
Summary: This study demonstrates the feasibility of using ex vivo confocal microscopy for the analysis of basic patterns of lesion in nephropathology. FuCM proves to be as effective as conventional techniques in evaluating acute tubular necrosis and interstitial fibrosis changes, facilitating rapid evaluation of kidney biopsies and restructuring the clinical workflow in renal histopathology.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Engineering, Chemical
Dharshana Nalatamby, Florence Gibouin, Javier Ordonez-Hernandez, Julien Renaudeau, Geirald Clisson, Norberto Farfan, Pierre Lidon, Yaocihuatl Medina-Gonzalez
Summary: Viscosity plays a determinant role in various industrial fluid processes and is especially important at the micro- and nanoscale. This study focuses on the use of fluorescent molecular rotors to quantitatively measure viscosity in confined and complex microfluidic flows. The technique is validated in controlled situations and then applied to passive micromixers to characterize and quantify mixing efficiency. This methodology provides a new approach for viscosity characterization in heterogeneous and complex systems.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Geochemistry & Geophysics
J. T. Sliwinski, H. M. Stoll
Summary: By analyzing stalagmites from Asturias, Spain using laser ablation inductively-coupled-plasma mass spectrometry and confocal laser scanning microscopy, seasonal banding and variations in trace elements and fluorescence were observed. Lateral fluorescence variations in saw-tooth patterns indicate disruptions in seasonal banding, which are corroborated by trace element differences such as Mg and Na. The findings suggest that caution should be taken when obtaining trace element information without prior reconnaissance mapping. Traditional drilling may homogenize lateral variations and provide more reliable trace element estimates.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Multidisciplinary Sciences
Ana-Maria Pena, Etienne Decenciere, Sebastien Brizion, Peggy Sextius, Serge Koudoro, Therese Baldeweck, Emmanuelle Tancrede-Bohin
Summary: This study proposes an approach combining multiphoton FLIM and Pseudo-FLIM to quantify the 3D melanin distribution in vivo. By comparing the analyses of synthetic melanin, melanocytes/keratinocytes coculture, and in vivo human skin, new insights into melanin modulation are provided.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Paulina Koziol, Karolina Kosowska, Danuta Liberda, Ferenc Borondics, Tomasz P. Wrobel
Summary: Infrared and Raman spectroscopies are commonly used to characterize the anisotropic structure of samples. By simultaneously analyzing two vibration bands, the orientation ordering in three dimensions can be determined.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemical Research Methods
Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M'Saad, Hannahmariam T. Mekbib, Fabian F. Voigt, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Joerg Bewersdorf, Reto Fiolka
Summary: The protocol provides detailed instructions for assembling and operating a versatile variant of light-sheet fluorescence microscopy called axially swept light-sheet microscopy (ASLM), which offers an unparalleled combination of field of view, optical resolution, and optical sectioning. It includes information on the working principle, applications, practical tips, part lists, schematics, and software for instrument control of ASLM.
Article
Multidisciplinary Sciences
Ayana Sugizaki, Keisuke Sato, Kazuyoshi Chiba, Kenta Saito, Masahiko Kawagishi, Yuri Tomabechi, Shalin B. Mehta, Hirokazu Ishii, Naoki Sakai, Mikako Shirouzu, Tomomi Tani, Sumio Terada
Summary: Biomolecular assemblies play a crucial role in cell physiology, with their function depending on the changes in molecular positions and orientations. Fluorescence polarization microscopy offers a promising solution by reporting the orientation of emission dipoles, providing insights into monitoring molecular orientation in real time within living cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Guankui Wang, Markella Zannikou, Laren Lofchy, Yue Li, Hanmant Gaikwad, Irina Balyasnikova, Dmitri Simberg
Summary: The type of fluorescent label on liposomes significantly impacts their extravasation and accumulation in tumors. In animal experiments, indocarbocyanine lipids (ICLs) were found to spread and accumulate in tumor tissue more easily compared to fluorescent phospholipids (FPLs).
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Article
Engineering, Biomedical
Julien Duboisset, Patrick Ferrand, Arthur Baroni, Tilman A. Grunewald, Hamadou Dicko, Olivier Grauby, Jeremie Vidal-Dupiol, Denis Saulnier, Le Moullac Gilles, Martin Rosenthal, Manfred Burghammer, Julius Nouet, Corinne Chevallard, Alain Baronnet, Virginie Chamard
Summary: Biomineralization is a complex process bio-controlled by living organisms, involving physical and chemical processes such as ionic concentration regulation and organic molecules production. This study investigates the early-mineralized calcareous units from two bivalve species using advanced optical and X-ray microscopy methods, revealing chemical and crystallographic insights. The observed ring-like structural features in calcite units are correlated with the absence of calcite and an increase in amorphous calcium carbonate and proteins content. Based on these findings, a temporal biomineralization cycle is proposed, providing biomimetic perspectives for designing crystalline materials.
ACTA BIOMATERIALIA
(2022)
Article
Parasitology
Hend H. A. M. Abdulla, Dina Aboelsoued, Tarek K. Fang, Sobhy Abdel-Shaft, Kadria N. Abdel Megeed, Philippe Parola, Didier Raoult, Oleg Mediannikov
Summary: This study investigated equine vector-borne diseases (EVBDs) in Egypt using molecular analyses, leading to the discovery of new pathogens such as Candidatus Ehrlichia rustica and a new Ehrlichia species. The study also identified a new Theileria species Africa, along with other pathogens in donkeys and horses.
Article
Biochemistry & Molecular Biology
Hamadou Dicko, Tilman A. Grunewald, Patrick Ferrand, Jeremie Vidal-Dupiol, Vaihiti Teaniniuraitemoana, Manaari Sham Koua, Gilles le Moullac, Jeremy Le Luyer, Denis Saulnier, Virginie Chamard, Julien Duboisset
Summary: In this study, a highly sensitive coherent Raman microscopy approach is used to image the molecular bond concentrations in the shell border. A novel method is presented to compare the amount of amorphous carbonate with its crystalline counterpart. The feasibility and reproducibility of the microscopy technique for imaging in vivo the shell border is demonstrated, opening new perspectives for chemical imaging in the study of biogenic and bio-inspired crystals.
JOURNAL OF STRUCTURAL BIOLOGY
(2022)
Article
Optics
David R. Smith, Siddarth Shivkumar, Jeff Field, Jesse W. Wilson, Herve Rigneault, Randy A. Bartels
Summary: Impulsive stimulated Raman scattering (ISRS) is a powerful technique for studying low-frequency Raman vibrational modes, and this study introduces a simple strategy for ISRS spectroscopy and hyperspectral imaging using spectral filters.
Article
Chemistry, Analytical
Clement Grand, Camille Scotte, Herve Rigneault
Summary: This study successfully applies compressive Raman imaging technology in the field of pharmacy. By incorporating a digital micromirror device and a single-pixel detector, fast chemometric analysis and microscopic imaging are achieved. The results demonstrate the potential of this technology for fast pharmaceutical tablet quality control imaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Sandro Heuke, Xavier Audier, Herve Rigneault
Summary: A double modulation scheme is proposed for stimulated Raman microscopy, where both the pump and Stokes beams are modulated at fm and 2fm. By exploiting aliasing and reduction of repetition rate, the proposed scheme amplifies the signal amplitude by a factor of 1.5, 2, and 4 for different modulation frequencies and experimental realizations at the same average power. The derived noise power for different sources shows that the double modulation scheme can achieve up to 16-fold speed improvement in stimulated Raman scattering imaging compared to single beam modulation.
Article
Chemistry, Physical
Romain Appay, Barbara Sarri, Sandro Heuke, Sebastien Boissonneau, Chang Liu, Etienne Dougy, Laurent Daniel, Didier Scavarda, Henry Dufour, Dominique Figarella-Branger, Herve Rigneault
Summary: Central nervous system tumors are a diverse group of neoplasms with different outcomes and treatment strategies. The classification of these tumors is now based on molecular parameters in addition to histopathology. Genomic characterization is crucial for identifying targeted therapies. Stimulated Raman histology (SRH) is a non-destructive imaging technique that allows for rapid examination of tissue samples without labeling or sectioning.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Optics
Zhouping Lyu, Ksenia Abrashitova, Johannes F. de Boer, Esben R. Andresen, Herve Rigneault, Lyubov Amitonova
Summary: In this study, a flexible probe based on a multicore-multimode fiber is proposed and experimentally demonstrated for sub-diffraction imaging. The probe consists of a multicore part with 120 single-mode cores and a multimode part for structured light illumination. The results show perturbation-resilient fast sub-diffraction fiber imaging by computational compressive sensing.
Article
Optics
Jeremy Saucourt, Antonin Moreau, Julien Lumeau, Herve Rigneault, Thomas Chaigne
Summary: Optical detection of ultrasound for photoacoustic imaging using Fabry-Perot cavity sensors allows for higher spatial resolutions compared to conventional piezoelectric detection. However, precise control of the interrogation beam wavelength is required for optimal sensitivity. This study proposes the use of a broadband source and a fast-tunable acousto-optic filter to adjust the interrogation wavelength at each pixel within microseconds, overcoming fabrication constraints and improving acquisition speed.
Article
Optics
Naveen Gajendra Kumar, Siddharth Sivankutty, Victor Tsvirkun, Andy Cassez, Damien Labat, Rosa Cossart, Geraud Bouwmans, Esben Ravn Andresen, Herve Rigneault
Summary: We present a modified fiber geometry for a bending-insensitive multi-core fiber (MCF) that allows optimal light coupling in and out of the individual cores, addressing the coupling complexity and potential degradation issues of previously reported twisted MCFs. By introducing a section with straight and parallel cores at the ends of the MCF, we rectify the coupling and output light problems, enabling the development of bend-insensitive lensless endoscopes.
Article
Physics, Applied
Siddarth Shivkumar, Dekel Ranann, Samuel Metais, Sisira Suresh, Nicolas Forget, Randy Bartels, Dan Oron, Herve Rigneault
Summary: Impulsive stimulated Raman scattering (ISRS) is a promising technique for vibrational imaging below 200 cm(-1) by using a single short femtosecond pump pulse. However, ISRS lacks vibrational selectivity as all vibrational bonds within the pump-pulse bandwidth are excited. In this work, we propose two pulse-shaping strategies for selective vibrational detection by shaping the probe pulse in time.
PHYSICAL REVIEW APPLIED
(2023)
Article
Optics
Camille Scotte, Frederic Galland, Herve Rigneault
Summary: In a single-pixel camera, the object is illuminated by intensity patterns and reconstructed computationally from the total reflected or transmitted intensity measured by a single-pixel detector. We investigate whether a single-pixel camera performs better than point raster scanning in the presence of photon-noise, and find that positive intensity modulation based on Hadamard or Cosine patterns does not necessarily improve the single-to-noise ratio (SNR) of single-pixel cameras. However, the SNR is improved on object pixels at least k times brighter than the object mean signal, where k depends on the modulation scheme.
JOURNAL OF PHYSICS-PHOTONICS
(2023)
Article
Engineering, Electrical & Electronic
D. Septier, D. Labat, A. Pastre, R. Bernard, G. Brevalle-Wasilewski, H. Rigneault, G. Bouwmans, A. Kudlinski
Summary: We designed, fabricated, and characterized the first hollow core double-clad fiber coupler for nonlinear micro-endoscopy. The coupler consists of a pure silica negative curvature hollow core double-clad fiber and a highly multimode fiber fused together. The use of a low fusion temperature soft (borosilicate) glass multimode fiber enabled the fabrication of this coupler without altering the geometry of the negative curvature fiber. A power transfer of 60% from the double-clad fiber to the multimode fiber was measured at 500 nm. The applicability of the coupler was demonstrated through two-photon fluorescence and second harmonic generation imaging of unlabeled biological tissues using an endoscopic head mounted on the hollow core double-clad fiber coupler. This device greatly simplifies the integration of nonlinear micro-endoscopes based on hollow core double clad fibers.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2023)
Article
Optics
Sandro Heuke, Carla Silva Martins, Remi Andre, Loic Legoff, Herve Rigneault
Summary: Two-photon excited fluorescence (2PEF) microscopy is a popular nonlinear imaging method for biomedical samples. It uses multiple detectors and spectral filter sets to discriminate different fluorophores based on their emission behavior. However, 2PEF suffers from the loss of fluorescence photons outside the filter transmission range and may fail to discriminate fluorophores with similar emission profiles. Here, we propose an alternative 2PEF method that discriminates fluorophores based on their excitation spectra.
Meeting Abstract
Chemistry, Multidisciplinary
C. Colas, T. Grunewald, F. Gobeaux, A. Campos, M. Burghammer, A. Baroni, P. Ferrand, V. Chamard, C. Chevallard
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2022)