Article
Multidisciplinary Sciences
Purnati Khuntia, Simran Rawal, Rituraj Marwaha, Tamal Das
Summary: The Golgi apparatus moves from an apical position to a basal position during epithelial cell migration, driven by actin cytoskeleton instead of microtubules. The interaction between Golgi and actin is mediated by actin projections, which is critical for cell migration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Kentaro Morikawa, Daichi Kuroda, Yasuhiro Inoue
Summary: Epithelial folding is a common phenomenon in morphogenesis, and the physical constraints imposed by the surrounding microenvironment play a critical role. This study investigates the effect of asymmetry on epithelial folding morphology using three-dimensional simulations and mechanical energy functional equations.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Julien Fierling, Alphy John, Barthelemy Delorme, Alexandre Torzynski, Guy Blanchard, Claire Lye, Gregoire Malandain, Benedicte Sanson, Jocelyn Etienne, Philippe Marmottant, Catherine Quilliet, Matteo Rauzi, Anna Popkova
Summary: Cell apical constriction driven by actomyosin contraction forces is a crucial mechanism in tissue folding during embryo development. This study challenges the idea that cell apical contraction forces alone are sufficient to drive tissue folding, and instead shows that a balance of forces at the tissue surface is necessary and responsible for initiating embryo gastrulation.
NATURE COMMUNICATIONS
(2022)
Article
Physics, Multidisciplinary
Surabhi Sonam, Lakshmi Balasubramaniam, Shao-Zhen Lin, Ying Ming Yow Ivan, Irina Pi-Jauma, Cecile Jebane, Marc Karnat, Yusuke Toyama, Philippe Marcq, Jacques Prost, Rene-Marc Mege, Jean-Francois Rupprecht, Benoit Ladoux
Summary: Epithelial cells are influenced by mechanical stress and abrasion, impacting their integrity. Culturing epithelial cells on two-dimensional hydrogels showed that soft substrates resulted in a loss of epithelial monolayer integrity through hole formation. This rupture was associated with cellular stretching and cell division events.
Article
Materials Science, Multidisciplinary
Bi-Cong Wang, Guang-Kui Xu
Summary: By considering the social interactions between adjacent cells and internal mechanical processes of the cell itself, we propose an active vertex model to study collective cell migration in geometrical constraints. Using this model, we can reproduce all reported motion modes of cells in circular constraints observed in existing experiments. Specifically, as the confinement size decreases, local swirling and global rotation modes successively appear in cell groups with finite correlation length, while eccentric rotation and coherent oscillation modes successively emerge in cell groups with scale-free correlations. These findings shed light on the spatiotemporal dynamics of active matter.
JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
(2023)
Article
Biochemical Research Methods
Sijie M. Tong, Navreeta K. Singh, Rastko M. Sknepnek, Andrej Kosmrlj
Summary: Epithelial tissues exhibit complex viscoelastic rheological behavior, where cells can actively tune their mechanical properties to change the overall response of the tissue. This study systematically investigated the shear and bulk responses of epithelia in both fluid and solid phases using small oscillatory shear and bulk deformations. The findings suggest that the solid-fluid transition can be controlled by applying pre-deformation to the system.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jie Bai, Xiaowei Zeng
Summary: Wounds in the epithelium can be self-repaired by the movement of epithelial cells, but the role of mechanics in wound closure is not well explained. A computational model considering mechanical properties of cells and intercellular interactions was developed to investigate the role of mechanics in wound closure. The model revealed that closure efficiency depends on the initial gap size and the intensity of lamellipodial protrusion. It also showed that cells at the wound edge experience higher stress and cellular normal stress dominates over shear stress. This model provides insights into the mechanical principles of wound closure.
SCIENTIFIC REPORTS
(2023)
Article
Biophysics
Evan C. Thomas, Sevan Hopyan
Summary: This study investigates the interplay between cell-level and tissue-level properties in influencing tissue behaviors, and proposes a phase diagram model with curvature and shape index to predict the behaviors of tissues under different conditions.
BIOPHYSICAL JOURNAL
(2023)
Article
Developmental Biology
Travis T. Parsons, Sheila Mosallaei, Laurel A. Raftery
Summary: During Drosophila oogenesis, somatic follicle cells differentiate and secrete components of the eggshell. A timeline of reproducible milestones for centripetal migration, which encloses the oocyte after secretion, is defined using time-lapse imaging of egg chamber explants. Inward migration occurs in two phases, with leading centripetal follicle cells ingressing over the anterior oocyte first and then followed by collective movement of the following cells towards the anterior. E-cadherin is required for normal ingression of the leading cells.
Review
Cell Biology
Ulla-Maj Fiuza, Patrick Lemaire
Summary: Gastrulation is a universal developmental process involving internalization of mesodermal and endodermal tissues and spreading of the ectoderm. Recent research highlights the importance of tissue rheological properties in regulating gastrulation. Ascidians, with invariant cell lineages and solid-like rheological behavior, provide insights into the mechanical and genetic control of gastrulation.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Adrien Mery, Artur Ruppel, Jean Revilloud, Martial Balland, Giovanni Cappello, Thomas Boudou
Summary: The authors engineer 3D microtissues from optogenetically modified fibroblasts and utilize light to induce local contractions and measure tissue stress and strain. They investigate the regulation and spatio-temporal distribution of these contractions, as well as the potential of light to create and map local anisotropies in mechanically heterogeneous microtissues. This study offers a new approach to guide tissue formation and non-destructively chart their rheology in real time using their own constituent cells as internal actuators.
NATURE COMMUNICATIONS
(2023)
Article
Physics, Multidisciplinary
Amit Das, Srikanth Sastry, Dapeng Bi
Summary: Cell neighbor exchanges play a crucial role in tissue rearrangements in biology, but these processes are rate limited in biological tissues compared to nonliving materials. This study shows how this time constraint affects the mechanics and collective behavior of cells in a tissue, leading to the development of spatially correlated populations of fast and slow cells. The predictions of this work are validated by existing in vivo experiments in Drosophila pupal development.
Article
Physics, Fluids & Plasmas
Souvik Sadhukhan, Saroj Kumar Nandi
Summary: Glassy dynamics in a confluent monolayer plays a crucial role in morphogenesis, wound healing, and other biological processes. Studying the theoretical framework for such systems is important, and a comprehensive understanding has been developed using numerical simulations and analytical studies in the cellular Potts model. The study shows that geometric constraints and target perimeter variations lead to distinct regimes in dynamics, revealing the unique characteristics of dynamics within the system.
Article
Multidisciplinary Sciences
Margherita De Marzio, Ayse Kilic, Enrico Maiorino, Jennifer Mitchel, Chimwemwe Mwase, Michael J. O'Sullivan, Maureen McGill, Robert Chase, Jeffrey J. Fredberg, Jin-Ah Park, Kimberly Glass, Scott T. Weiss
Summary: By conducting compression experiments on bronchial epithelial cells, the genome-wide analysis of UJT revealed different activation stages of intracellular and extracellular molecular networks induced by compression. Furthermore, the study found common genomic patterns associated with ECM remodeling in nonasthmatic and asthmatic bronchial epithelial cells.
Article
Engineering, Biomedical
Adrian Cervero-Varona, Angelo Canciello, Alessia Peserico, Arlette Alina Haidar Montes, Maria Rita Citeroni, Annunziata Mauro, Valentina Russo, Samanta Moffa, Serena Pilato, Stefano Di Giacomo, Beatrice Dufrusine, Enrico Dainese, Antonella Fontana, Barbara Barboni
Summary: The use of biomaterials in immune regenerative strategies for unresolved pathologies is gaining attention in tissue engineering. Graphene oxide (GO) has been proposed as an immune-mimetic material for stem cell-based regenerative therapies, influencing stem cell behavior and immune response. Amniotic epithelial stem cells (AECs) are also of interest due to their plasticity and immunomodulatory paracrine activities, and the effects of GO on AECs are explored in this study. GO-coating induced and accelerated the Epithelial-Mesenchymal Transition (EMT) in AECs, leading to changes in cytokine secretion and enhanced activation of macrophages. Adhesion of AECs on GO-functionalized surfaces could create a tailored microenvironment for the regenerative process.
MATERIALS TODAY BIO
(2023)
Article
Immunology
Baubak Bajoghli, Paola Kuri, Daigo Inoue, Narges Aghaallaei, Marleen Hanelt, Thomas Thumberger, Matteo Rauzi, Joachim Wittbrodt, Maria Leptin
JOURNAL OF IMMUNOLOGY
(2015)
Article
Multidisciplinary Sciences
Joichiro Nakakura, Primoz Ziherl, Junichi Matsuzawa, Tomonari Dotera
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Gustavo de Medeiros, Dimitri Kromm, Balint Balazs, Nils Norlin, Stefan Guenther, Emiliano Izquierdo, Paolo Ronchi, Shinya Komoto, Uros Krzic, Yannick Schwab, Francesca Peri, Stefano de Renzis, Maria Leptin, Matteo Rauzi, Lars Hufnagel
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Anna Popkova, Orrin J. Stone, Lin Chen, Xiang Qin, Chang Liu, Jiaying Liu, Karine Belguise, Denise J. Montell, Klaus M. Hahn, Matteo Rauzi, Xiaobo Wang
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Jan Rozman, Matej Krajnc, Primoz Ziherl
NATURE COMMUNICATIONS
(2020)
Review
Biology
Matteo Rauzi
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Review
Cell Biology
Anna Popkova, Matteo Rauzi, Xiaobo Wang
Summary: Tissue elongation is driven by tissue rotation and actomyosin contractions in Drosophila egg chamber, where supracellular networks constrain egg growth in a polarized manner. Both cellular and supracellular planar polarity play critical roles in controlling tissue extension.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Alphy John, Matteo Rauzi
Summary: In the Drosophila embryo, mesoderm cells fold and extend simultaneously under the synergistic control of anterior-posterior and dorsal-ventral gene patterning, driving tissue growth and shape changes.
DEVELOPMENTAL CELL
(2021)
Article
Chemistry, Physical
Jan Rozman, Matej Krajnc, Primoz Ziherl
Summary: Using a three-dimensional active vertex model, this study numerically investigates the shapes of strained unsupported epithelial monolayers subject to active junctional noise. The research reveals that different types of compressive strains lead to different fold morphologies, and villus morphology appears only when junctional tension fluctuations are strong enough to fluidize the tissue. Additionally, fluidized epithelium can form villi even in the absence of compressive strain under large apico-basal differential surface tension.
EUROPEAN PHYSICAL JOURNAL E
(2021)
Review
Cell Biology
Alphy John, Matteo Rauzi
Summary: Morphogenesis drives the formation of living shapes through gene expression patterns and signaling pathways. While previous studies have focused on simple morphogenetic processes, recent research has begun to explore how tissues can undergo multiple simultaneous changes in shape, particularly in the context of tissue folding and extension during embryonic development. Pioneering studies have highlighted the role of spatially coordinated cell mechanisms and gene expression patterns in driving composite morphogenesis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Julien Fierling, Alphy John, Barthelemy Delorme, Alexandre Torzynski, Guy Blanchard, Claire Lye, Gregoire Malandain, Benedicte Sanson, Jocelyn Etienne, Philippe Marmottant, Catherine Quilliet, Matteo Rauzi, Anna Popkova
Summary: Cell apical constriction driven by actomyosin contraction forces is a crucial mechanism in tissue folding during embryo development. This study challenges the idea that cell apical contraction forces alone are sufficient to drive tissue folding, and instead shows that a balance of forces at the tissue surface is necessary and responsible for initiating embryo gastrulation.
NATURE COMMUNICATIONS
(2022)
Article
Physics, Multidisciplinary
Urska Andrensek, Primoz Ziherl, Matej Krajnc
Summary: We investigate the elasticity of an unsupported epithelial monolayer and discover that it may wrinkle even in the absence of the underlying substrate. We derive an exact elasticity theory from a cell-based model and find that wrinkling is driven by the differential apico-basal surface tension. By introducing a phantom substrate with finite stiffness beyond a critical tension, our theory is mapped onto that for supported plates, suggesting a new mechanism for tissues to control their surface patterns autonomously.
PHYSICAL REVIEW LETTERS
(2023)
Article
Chemistry, Physical
Toshikaze Chiba, Yuka Sakuma, Masayuki Imai, Primoz Ziherl
Summary: We studied the morphological changes of adhering vesicle triplets under different adhesion regimes and analyzed their dependence on volume-to-area ratio. In the strong adhesion regime, the triplet morphology varied based on the arrangement of vesicles. Decreasing the volume-to-area ratio transformed a triangular triplet into a compact spherical shape with a sigmoidal contact zone, while a linear vesicle triplet deformed into a compact spherical shape with biconvex interfaces. The morphologies of triplets with flat contact zones were explained by the two-tension model, but when the interface deformed, the cavity model was more appropriate. In the weak adhesion regime, both triangular and linear vesicle triplets transformed into prolate-based triplets by reducing the volume-to-area ratio.
Article
Physics, Fluids & Plasmas
M. Kokalj Ladan, P. Ziherl, A. Siber
Article
Chemistry, Physical
B. Kavcic, A. Sakashita, H. Noguchi, P. Ziherl