Article
Nanoscience & Nanotechnology
Lukasz Bijoch, Urszula Wlodkowska, Rafal Kasztelanic, Monika Pawlowska, Dariusz Pysz, Leszek Kaczmarek, Radek Lapkiewicz, Ryszard Buczynski, Rafal Czajkowski
Summary: In this study, we provide experimental verification for the use of short-section imaging bundles in two-photon microscopy imaging of the mouse brain. The bundle, made of heavy-metal oxide glasses, has a high numerical aperture and allows for successful imaging with high resolution. The system can be used for minimal-invasive in vivo imaging of various brain areas and is cost-effective and easy to operate.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Guanghan Meng, Jian Zhong, Qinrong Zhang, Justin S. J. Wong, Jianglai Wu, Kevin K. Tsia, Na Ji
Summary: Understanding blood flow dynamics is crucial for studying the function of blood vessels. Current methods lack the resolution to monitor blood flow at a cellular level in large vessels. However, using a fast line-scanning module, researchers were able to achieve high-resolution imaging of blood flow in the cortex of awake mice. This breakthrough allowed them to measure cerebral blood flow and observe pulsatile blood flow patterns.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Yi Chen
Summary: This review highlights the use of A beta-specific fluorescent probes for two-photon imaging of A beta plaques in vivo. The synthesis and detection mechanism of these probes, as well as their application in imaging A beta plaques in vivo, are discussed.
Article
Chemistry, Multidisciplinary
M. Marini, A. Nardini, R. Martinez Vazquez, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, M. T. Raimondi, G. Chirico
Summary: Non-linear excitation microscopy has advantages over conventional confocal techniques for in-vivo imaging, but tissue penetration can still be problematic. This study develops and tests fibroblast cell culture plano-convex microlenses for non-linear imaging of biological tissue. The microlenses can be used individually or in an array, and they provide magnified fluorescence images without substantially affecting the signal-to-noise ratio. These results pave the way for optical in-vivo inspection of biological processes using implanted micro-optics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Xueyang Xiao, Hao Cai, Qiaorong Huang, Bing Wang, Xiaoming Wang, Qiang Luo, Yinggang Li, Hu Zhang, Qiyong Gong, Xuelei Ma, Zhongwei Gu, Kui Luo
Summary: In this study, a dual-modality contrast agent, Gd-DOTA-TPBP, was successfully synthesized using an amphiphilic block polymer as a carrier. The contrast agent exhibited high resolution and sensitivity, making it a promising tool for early-stage tumor diagnosis.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Huifang Su, Tao Xie, Yong U. Liu, Yuhan Cui, Wei Wen, Ben Zhong Tang, Wei Qin
Summary: The application of fluorescent probes for in vivo retinal imaging is crucial for understanding eye diseases. A group of organic luminogens with electron-donating and electron-accepting structures were synthesized and found to be efficient emitters. Their resulting fluorescent nanoparticles showed excellent performance as lipid droplets targeting probes. Utilizing these nanoparticles, accurate and detailed imaging of live zebrafish retina was achieved for the first time using two-photon fluorescence microscopic technique.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Shaowei Wang, Xueqi Li, Suet Yen Chong, Xiaoyuan Wang, Huan Chen, Chengjian Chen, Lai Guan Ng, Jiong-Wei Wang, Bin Liu
Summary: Organic dots with aggregation-induced emission (AIEgen) have been developed for in vivo labeling and imaging of lipid-rich tissues, showing high targeting specificity and stability, as well as strong three-photon fluorescence imaging in deep tissues under NIR-II laser excitation. This study will inspire the development of lipid-targeting fluorophores for in vivo applications.
ADVANCED MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Nuzhet I. Kilic, Giovanni M. Saladino, Sofia Johansson, Rickard Shen, Cacie Mcdorman, Muhammet S. Toprak, Stefan Johansson
Summary: Two-photon polymerization (2PP) is an efficient technique for high-resolution, three-dimensional printing of complex structures. However, it is limited by the choice of photocurable monomers. This study proposes a solution by integrating metallic Rh NPs, which have low laser-induced interactions and increased dispersibility threshold, into the photopolymer resin. The Rh NPs also have potential applications as contrast agents for X-ray fluorescence bioimaging. The results demonstrate the ability to achieve higher concentrations and highlight the potential use of NP-loaded 3D-printed nanostructures for medical devices.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Instruments & Instrumentation
Jintao Xia, Afei Ding, Pan Wang, Hang Wang, Yinwei Gu, Weidong Tao, Gang Wang
Summary: This paper proposes a planar micro-assembly technology to fabricate delicate 3D structures and assemble fluorescent powder and graphene powder into microstructures. The properties of the powders remain unchanged, and the experimental device allows for the formation of complex hybrid microstructure systems with different materials and characteristics.
Article
Nanoscience & Nanotechnology
Wenxuan Liang, Defu Chen, Honghua Guan, Hyeon-Cheol Park, Kaiyan Li, Ang Li, Ming-Jun Li, Israel Gannot, Xingde Li
Summary: This article introduces a newly developed two-photon fluorescence lifetime imaging endomicroscope that can extract NADH lifetime parameters of living tissues and organs, track changes in cellular metabolic status and tumor models, and promises real-time dynamic imaging in clinical diagnosis and therapeutic applications.
Article
Chemistry, Multidisciplinary
Shen Tong, Jincheng Zhong, Xinlin Chen, Xiangquan Deng, Jie Huang, Yingxian Zhang, Mengyuan Qin, Zhenhui Li, Hui Cheng, Wanjian Zhang, Lei Zheng, Weixin Xie, Ping Qiu, Ke Wang
Summary: Multiphoton microscopy (MPM) is a powerful technique for visualizing deep-brain structures at high resolution. This study demonstrates the measurement technology for the multiphoton excitation and emission properties of fluorescent labels at the 2200 nm window, and successfully applies it to deep-brain vasculature imaging in vivo.
Article
Biochemical Research Methods
Po-Yen Lin, Sheng-Ping L. Hwang, Chi-Hon Lee, Bi-Chang Chen
Summary: By incorporating axicon imaging into LSFM, the depth of field is extended, allowing for faster three-dimensional imaging and capturing dynamic changes in physiological processes.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Multidisciplinary
Ruizhe Chen, Yonghui Pan, Zizi Wu, Mingxuan Jia, Xiaofei Miao, Xiaomei Lu, Quli Fan, Wenbo Hu
Summary: Developing photostable two-photon dyes with high brightness and negligible photocytotoxicity is crucial but challenging for fluorescence imaging.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Polymer Science
Martyna Durko-Maciag, Gilles Ulrich, Julien Massue, Jaroslaw Mysliwiec, Konrad Cyprych
Summary: This work demonstrates the successful application of Excited-State Intramolecular Proton Transfer (ESIPT) fluorophores as photoinitiators for microfabrication. The influence of chemical structure on this application was investigated, followed by analysis of two-photon polymerization. The study determined the quality of fabricated structures and polymerization thresholds, with one of the chromophores having an impressively low value of 11 nJ.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Spectroscopy
Feng-Tao Yu, Ze Huang, Jia-Xiang Yang, Long-Mei Yang, Xian-Yun Xu, Jian-Yan Huang, Lin Kong
Summary: Two viscosity-sensitive fluorescent probes were designed and synthesized to localize in lysosome and mitochondria in living HeLa cells, respectively. The fluorescence quantum yield and two-photon absorption cross section of the probes were significantly increased with the increase of viscosity. Fluorescence lifetime imaging of the cells stained with the probes revealed the viscosity range of lysosome and mitochondria. This type of probe is of great importance for monitoring diseases associated with abnormal viscosity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Neurosciences
Arnau Busquets-Garcia, Jose F. Oliveira da Cruz, Geoffrey Terral, Antonio C. Pagano Zottola, Edgar Soria-Gomez, Andrea Contini, Hugo Martin, Bastien Redon, Marjorie Varilh, Christina Ioannidou, Filippo Drago, Federico Massa, Xavier Fioramonti, Pierre Trifilieff, Guillaume Ferreira, Giovanni Marsicano
Review
Neurosciences
Andry Andrianarivelo, Estefani Saint-Jour, Roman Walle, Pierre Trifilieff, Peter Vanhoutte
Article
Endocrinology & Metabolism
Fabien Ducrocq, Alexia Hyde, Hortense Fanet, Asma Oummadi, Roman Walle, Veronique De Smedt-Peyrusse, Sophie Laye, Guillaume Ferreira, Pierre Trifilieff, Sylvie Vancassel
Article
Chemistry, Analytical
Roberto Fernandez, Jone Garate, Tarson Tolentino-Cortez, Ainara Herraiz, Laura Lombardero, Fabien Ducrocq, Rafael Rodriguez-Puertas, Pierre Trifilieff, Egoitz Astigarraga, Gabriel Barreda-Gomez, Jose A. Fernandez
ANALYTICAL CHEMISTRY
(2019)
Review
Neurosciences
Marine Salery, Pierre Trifilieff, Jocelyne Caboche, Peter Vanhoutte
BIOLOGICAL PSYCHIATRY
(2020)
Article
Behavioral Sciences
H. Fanet, F. Ducrocq, M. Tournissac, A. Oummadi, A. Lo, P. Bourrassa, V De Smedt-Peyrusse, B. Azzougen, L. Capuron, S. Laye, F. Moussa, P. Trifilieff, F. Calon, S. Vancassel
BEHAVIOURAL BRAIN RESEARCH
(2020)
Article
Biochemical Research Methods
Ying Zhu, Jozsef Meszaros, Roman Walle, Rongxi Fan, Ziyi Sun, Andrew J. Dwork, Pierre Trifilieff, Jonathan A. Javitch
Meeting Abstract
Clinical Neurology
H. Martin, S. Bullich, F. Ducrocq, P. Trifilieff, S. Laye, B. Guiard, X. Fioramonti
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2019)
Article
Cell Biology
Fabien Ducrocq, Roman Walle, Andrea Contini, Asma Oummadi, Baptiste Caraballo, Suzanne van der Veldt, Marie-Lou Boyer, Frank Aby, Tarson Tolentino-Cortez, Jean-Christophe Helbling, Lucy Martine, Stephane Gregoire, Stephanie Cabaret, Sylvie Vancassel, Sophie Laye, Jing Xuan Kang, Xavier Fioramonti, Olivier Berdeaux, Gabriel Barreda-Gomez, Elodie Masson, Guillaume Ferreira, David W. L. Ma, Clementine Bosch-Bouju, Veronique De Smedt-Peyrusse, Pierre Trifilieff
Article
Cell Biology
Alice Shaam Al Abed, Azza Sellami, Mylene Potier, Eva-Gunnel Ducourneau, Pauline Gerbeaud-Lassau, Laurent Brayda-Bruno, Valerie Lamothe, Nathalie Sans, Aline Desmedt, Peter Vanhoutte, Catherine Bennetau-Pelissero, Pierre Trifilieff, Aline Marighetto
Editorial Material
Medicine, Research & Experimental
Fabien Ducrocq, Pierre Trifilieff
M S-MEDECINE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Andry Andrianarivelo, Estefani Saint-Jour, Paula Pousinha, Sebastian P. Fernandez, Anna Petitbon, Veronique De Smedt-Peyrusse, Nicolas Heck, Vanesa Ortiz, Marie-Charlotte Allichon, Vincent Kappes, Sandrine Betuing, Roman Walle, Ying Zhu, Charlene Josephine, Alexis-Pierre Bemelmans, Gustavo Turecki, Naguib Mechawar, Jonathan A. Javitch, Jocelyne Caboche, Pierre Trifilieff, Jacques Barik, Peter Vanhoutte
Summary: The study reveals that addictive drugs induce heteromerization of dopamine receptors with glutamate NMDA receptors, playing a key role in cocaine-induced adaptive changes. Blocking the formation of heteromers between dopamine receptor D2R and NMDA receptor can prevent the persistence of these adaptive changes.
Review
Neurosciences
Marie-Charlotte Allichon, Vanesa Ortiz, Paula Pousinha, Andry Andrianarivelo, Anna Petitbon, Nicolas Heck, Pierre Trifilieff, Jacques Barik, Peter Vanhoutte
Summary: Drug addiction is characterized by compulsive drug-seeking and drug-taking behavior, with recurrent cycles of abstinence and relapse despite negative consequences. Dopamine and glutamate play crucial roles in shaping the neural circuits involved in reward processing and addiction. Research on how dopamine and glutamate modulate specific inputs onto different neuronal populations within the reward circuitry sheds light on the molecular mechanisms underlying pathological behaviors associated with drug addiction. Such cell-type-specific molecular studies hold potential for developing innovative strategies for addiction treatment.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Vincent Paget-Blanc, Marlene E. Pfeffer, Marie Pronot, Paul Lapios, Maria-Florencia Angelo, Roman Walle, Fabrice P. Cordelieres, Florian Levet, Stephane Claverol, Sabrina Lacomme, Melina Petrel, Christelle Martin, Vincent Pitard, Veronique De Smedt Peyrusse, Thomas Biederer, David Perrais, Pierre Trifilieff, Etienne Herzog
Summary: Dopamine is an important regulator of brain function, and its transmission at dopamine hub synapses may synergize with other neurotransmitters. This study used targeted labeling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes and identified 57 specifically enriched proteins. Additionally, the adhesion of dopaminergic synapses to glutamatergic, GABAergic, or cholinergic synapses was observed, supporting local dopaminergic signaling.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Marie-Lise Jobin, Veronique De Smedt-Peyrusse, Fabien Ducrocq, Rim Baccouch, Asma Oummadi, Maria Hauge Pedersen, Brian Medel-Lacruz, Maria-Florencia Angelo, Sandrine Villette, Pierre Van Delft, Laetitia Fouillen, Sebastien Mongrand, Jana Selent, Tarson Tolentino-Cortez, Gabriel Barreda-Gomez, Stephane Gregoire, Elodie Masson, Thierry Durroux, Jonathan A. Javitch, Ramon Guixa-Gonzalez, Isabel D. Alves, Pierre Trifilieff
Summary: Increasing evidence suggests a connection between lipid metabolism and mental health, particularly in relation to polyunsaturated fatty acids (PUFAs). Recent findings show that PUFA levels in the brain are linked to dopamine transmission, a key system involved in psychiatric symptoms. However, the underlying mechanisms of this relationship are still unclear.
MOLECULAR PSYCHIATRY
(2023)
