期刊
BIOPHYSICAL JOURNAL
卷 102, 期 8, 页码 L34-L36出版社
CELL PRESS
DOI: 10.1016/j.bpj.2012.03.011
关键词
-
类别
资金
- American Heart Association [0635125N, 11GRNT7270014]
- University at Albany, State University of New York
TWIK-1 two-pore domain K+ channels generally produce nonmeasurable or very low levels of K+ currents in heterologous expression systems under physiologically ionic conditions. Two controversial mechanisms have been proposed to account for this behavior: TWIK-1 K+ channels are expressed in the cell surface but silenced by sumoylation at a lysine residue (TWIK-1 K274); constitutive and rapid internalization of TWIK-1 causes TWIK-1 channel silencing. Here we report that TWIK-1 K+ channels heterologously expressed in Chinese hamster ovary cells, which are silent in physiological K+ gradients, are able to conduct large monovalent cation currents when extracellular ionic conditions change. These results support the hypothesis that TWIK-1 K+ channels are expressed in the cell surface but silent, and suggest that the TWIK-1 gating behavior rather than the lack of cell surface expression of TWIK-1 results in nondetectable TWIK-1 K+ currents in heterologous expression systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据