Article
Materials Science, Multidisciplinary
Tun Naw Sut, Bo Kyeong Yoon, Won-Yong Jeon, Joshua A. Jackman, Nam-Joon Cho
Summary: Supported lipid bilayer (SLB) coatings, versatile cell-membrane-mimicking biointerfaces, have been expanded for medical and biotechnology applications, facilitating various studies and applications like biosensors, diagnostics, and antifouling surfaces.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Multidisciplinary
Rashad Kariuki, Rowan Penman, Saffron J. Bryant, Rebecca Orrell-Trigg, Nastaran Meftahi, Russell J. Crawford, Chris F. McConville, Gary Bryant, Kislon Voitchovsky, Charlotte E. Conn, Andrew J. Christofferson, Aaron Elbourne
Summary: Nanomaterials have the potential to revolutionize biological and biomedical research, and this study provides detailed insights into how gold nanoparticles interact with phospholipid membranes. The study shows that the nanoparticles can be absorbed and internalized by fluid-phase membranes, and partially embed into gel-phase membranes, causing perturbations throughout the bilayers.
Article
Nanoscience & Nanotechnology
Nicolo Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. . Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marite Cardenas
Summary: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We investigated the structure of lipid bilayers formed on nanoparticle arrays through vesicle fusion using scattering techniques and direct imaging. The presence of both flat and curved supported lipid bilayers on the same substrate, combined with the accuracy of grazing incidence neutron scattering, offers a promising approach to studying curvature-dependent membrane phenomena.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Emmanuelle Bignon, Marco Marazzi, Stephanie Grandemange, Antonio Monari
Summary: This study investigates the structural properties of the non-structural protein 6 (Nsp6) of SARS-CoV-2 using long-timescale molecular dynamics simulations and machine learning analysis. The findings reveal the interaction patterns of Nsp6 with the lipid membrane and suggest that the mutation brought by the Omicron variant may alter specific interactions, particularly aiding in anchoring the viral protein to the lipid bilayer interface.
Article
Nanoscience & Nanotechnology
Guilherme B. Berselli, Aurelien Gimenez, Alexandra O'Connor, Tia E. Keyes
Summary: A biomolecular photoactive device based on light-activated proton pump bR reconstituted into highly fluidic microcavity-supported lipid bilayers (MSLBs) has been developed, demonstrating efficient photoelectrical properties. The optimal conditions for a self-sustaining photoelectrical switch were determined, showing significant increase in transient current for lipid bilayers containing approximately 0.3 mol % bR with a measured photo-current of 250 nA/cm(2).
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Surajit Ghosh, Zeinab Mohamed, Jung-Ho Shin, Samavi Farnush Bint E. Naser, Karan Bali, Tobias Dorr, Roisin M. Owens, Alberto Salleo, Susan Daniel
Summary: Antibiotic resistance is a global health concern due to the decreasing number of available antibiotics and the development of drug-resistant bacteria. Understanding the interactions between the bacterial membrane and antibiotics is crucial for designing novel antimicrobial drugs. By creating a surface-supported planar bacterial outer membrane model, researchers can study these interactions and gain insights into membrane properties for drug design.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Olivia D. Walsh, Leona Choi, Krishna P. Sigdel
Summary: Antimicrobial peptides play a crucial role in the immune system and can affect membrane integrity. This study used atomic force microscopy to investigate the effect of a synthetic hybrid peptide CM15 on the lipid bilayer of E. coli. It was found that CM15 quickly formed membrane-spanning defects and disrupted membrane integrity at high concentrations.
Article
Biochemical Research Methods
Mohtadin Hashemi, Yuri L. Lyubchenko
Summary: A hybrid resolution molecular dynamics simulation method has been developed to investigate the interaction of amyloid proteins with lipid membranes, accelerating the aggregation propensity of amyloid proteins. This method allows for routine and accurate simulation of multi-protein and complex membrane systems, mimicking biologically relevant lipid membranes on microsecond time scales. It has been successfully applied to model the interactions of alpha-synuclein protein with a mixed lipid bilayer.
Article
Biochemical Research Methods
Siddhartha Banerjee, Yuri L. Lyubchenko
Summary: The article discusses the preparation of smooth, stable, and structurally homogeneous lipid bilayers without trapped vesicles, for probing the time-dependent early stages of aggregation of monomeric amyloid proteins. This methodology development allows for monitoring amyloid protein aggregation at low protein concentrations, and for studying the specific role of different lipids in aggregation kinetics and disease development mechanisms.
Article
Biochemistry & Molecular Biology
Kathleen W. Swana, Terri A. Camesano, Ramanathan Nagarajan
Summary: Supported lipid bilayers (SLBs) on quartz crystals are versatile model systems for studying cell membrane behavior. This study successfully formed entirely anionic SLBs by treating the quartz crystal, which provides valuable model systems for studying interactions with Gram-positive bacterial membranes.
Article
Chemistry, Physical
Lorena Ruano, Gustavo Cardenas, Juan J. Nogueira
Summary: This study investigates the intermolecular interactions between platinum-based anticancer drugs and lipid bilayers, finding that the permeation of cisplatin through a model membrane involves complex interactions with the polar and non-polar regions of the lipid bilayer, influenced by long-range electrostatic and hydrogen bond interactions. The permeation free-energy profile is determined by a balance between drug/lipid interactions, dehydration of the drug, and changes in membrane ordering along the permeation pathway.
Article
Biochemistry & Molecular Biology
Georgina Csizmadia, Gabor Erdos, Hedvig Tordai, Rita Padanyi, Silvio Tosatto, Zsuzsanna Dosztanyi, Tamas Hegedus
Summary: Protein and lipid membrane interactions are vital for cellular processes, where Intrinsically Disordered Protein Regions (IDRs) play key roles. MemMoRFs are IDRs involved in disorder-to-order transitions induced by membrane lipids. Characterization of the dynamics of MemMoRFs involves secondary structure propensity and flexibility calculated from nuclear magnetic resonance chemical shifts.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Analytical
Karutha Pandian Divya, Shanmuganathan Keerthana, Chinnuswamy Viswanathan, Nagamony Ponpandian
Summary: This paper reports a biomimetic bilayer lipid membrane supported MXene based biosensor for electrochemical hybridization detection of the most prevalent and potential breast cancer biomarker BRCA1. A 2D MXene nanosheet-anchored gold nanoparticle-decorated biomimetic bilayer lipid membrane (AuNP@BLM) biosensor is used for the attachment of thiolated single-stranded DNA (HS-ssDNA) targeting hybridization detection. The synergistic combination of MXene and AuNP@BLM has proven to efficiently improve the detection signal to several folds.
Article
Nanoscience & Nanotechnology
Hyunhyuk Tae, Soohyun Park, Gamaliel Junren Ma, Nam-Joon Cho
Summary: In this study, an air-stable supported lipid bilayer (SLB) that is laterally mobile upon rehydration was fabricated using a sucrose-bicelle complex system. The sucrose fraction in the system was found to be an important factor, with 20 wt% sucrose yielding SLBs that fully recovered lateral mobility after the dehydration-rehydration process. This research provides potential for a wide range of biointerfacial science applications.
Article
Chemistry, Multidisciplinary
Anurag Sharma, Geetanjali Negi, Monika Chaudhary, Nagma Parveen
Summary: In this study, we investigated how ganglioside composition affects the kinetics of supported lipid bilayer (SLB) formation using the vesicle rupturing method on a solid surface. We observed the attachment of vesicles and the subsequent SLB formation using time-lapse total internal reflection fluorescence microscopy. The type and concentration of gangliosides were found to influence the adsorption kinetics of vesicles and their lifetime on the surface before rupturing. Additionally, the simultaneous rupturing of surface-adsorbed vesicles formed microscopic lipid patches on the surface, which was triggered by a critical coverage of vesicles independent of their composition.
Article
Chemistry, Multidisciplinary
Can Gao, Atul Shukla, Haikuo Gao, Zhagen Miao, Yihan Zhang, Pu Wang, Guiwen Luo, Yi Zeng, Wallace W. H. Wong, Trevor A. Smith, Shih-Chun Lo, Wenping Hu, Ebinazar B. Namdas, Huanli Dong
Summary: Organic light-emitting transistors (OLETs) have demonstrated great potential applications in various fields as highly integrated and minimized optoelectronic devices. However, the construction of high-performance OLETs remains a significant long-term challenge, especially for single component active layer OLETs. In this study, the successful harvesting of triplet excitons in a high mobility emissive molecule, 2,6-diphenylanthracene (DPA), through the triplet-triplet annihilation process is demonstrated.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Anjay Manian, Rohan J. Hudson, Pria Ramkissoon, Trevor A. Smith, Salvy P. Russo
Summary: This benchmarking study investigates non adiabatic matrix coupling elements (NACMEs) using different density functionals, with a focus on the photophysical properties of perylene in toluene. The study compares theoretical calculations with experimental results, deriving relations between TDDFT and DFT/MRCI properties and identifying the most promising data sets.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2023)
Article
Physics, Multidisciplinary
Subhajit Chakraborty, Yang Xu, Ann Roberts, Debabrata Goswami, Trevor A. Smith
Summary: Evanescent wave-induced fluorescence spectroscopy (EWIFS) is a widely used technique for studying complex media in physical, chemical, and biological sciences. It utilizes the evanescent wave generated by total internal reflection to probe the photoluminescence characteristics of the sample. However, it faces limitations in imaging samples with higher refractive indices.
Article
Chemistry, Multidisciplinary
Michael Wilms, Lesly V. Melendez, Rohan J. Hudson, Christopher R. Hall, Samantha Prabath Ratnayake, Trevor Smith, Enrico Della Gaspera, Gary Bryant, Timothy U. Connell, Daniel E. Gomez
Summary: We introduce a novel composite material composed of silver nanoparticles and three-dimensional molecular organic cages based on light-absorbing porphyrins. The porphyrin cages not only stabilize the particles but also enable diffusion and trapping of small molecules near the metallic surface. The combination of these two photoactive components leads to a Fano-resonant interaction, resulting in increased energy transfer and enhanced photocurrent for water-splitting applications. This composite structure demonstrates the potential of advanced photosensitizer systems with intrinsic porosity for photocatalytic and sensing applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Medicine, Research & Experimental
Fariha Alam, Tracey A. Gaspari, Barbara K. Kemp-Harper, Edward Low, Aaron Aw, Dorota Ferens, Iresha Spizzo, Ann-Maree Jefferis, Praveen Praveen, Robert E. Widdop, Ross A. D. Bathgate, Mohammed Akhter Hossain, Chrishan S. Samuel
Summary: This study found that B7-33 can retain the cardioprotective effects of RLX and has a better therapeutic effect compared to the ACE inhibitor perindopril. Therefore, B7-33 could be considered as a cost-effective cardioprotective therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Medicinal
Mohammed Akhter Hossain, Praveen Praveen, Nurhayati Ahmad Noorzi, Hongkang Wu, Ian P. Harrison, Thomas Handley, Stavros Selemidis, Chrishan S. Samuel, Ross A. D. Bathgate
Summary: H2 relaxin is a peptide hormone that acts through the RXFP1 receptor and has important biological functions. Overexpression of H2 relaxin and RXFP1 in prostate cancer suggests their potential as targets for inhibiting tumor growth. In this study, a novel high-affinity RXFP1 antagonist, H2 B-R13HR, was developed and shown to inhibit relaxin-mediated tumor growth in a mouse model. This compound provides insights into relaxin actions through RXFP1 and may have therapeutic potential for prostate cancer.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Rong Li, Thomas N. G. Handley, Wenyi Li, Neil M. O'Brien-Simpson, Mohammed Akhter Hossain, John D. Wade
Summary: Antimicrobial resistance is increasing and causing 700,000 deaths worldwide in 2020. Antimicrobial peptides are effective against drug-resistant pathogens and have a lower risk of developing resistance. Dimerisation of antimicrobial peptides through disulfide bonds can enhance antimicrobial activity and reduce toxicity.
AUSTRALIAN JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Physical
Anjay Manian, Francesco Campaioli, Rohan J. Hudson, Jared H. Cole, Timothy W. Schmidt, Igor Lyskov, Trevor A. Smith, Salvy P. Russo
Summary: The role of charge transfer states in multi-exciton mechanisms is difficult to model accurately, but the intermolecular packing has been shown to heavily influence these mechanisms. By studying a gas phase perylene dimer, it was found that displaced geometries yield large charge transfer contributions for singlet fission, while triplet-triplet annihilation charge transfer couplings are weak. Slipping of the dimer cofacial geometry is beneficial to both charge transfer-mediated processes within a wavefunction overlap scheme. The results provide insights into singlet fission and can guide further investigations in this field.
CHEMISTRY OF MATERIALS
(2023)
Article
Chemistry, Physical
Trevor A. Smith, Rohan J. Hudson, Anjay Manian, Christopher R. Hall, Timothy W. Schmidt, Salvy P. Russo, Kenneth P. Ghiggino
Summary: Gating logical operations through high-lying excited states of perylene has potential for the development of ultrafast, subnanometer computational devices. This study uses femtosecond spectroscopy and quantum chemical calculations to investigate the relaxation dynamics of monomeric perylene's higher electronic excited states. The obtained lifetimes of the 2(1)Ag and 2(1)B(2u) states suggest that these states could be useful for logical operations.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Yixiong Ji, Wangxian Chen, Di Yan, James Bullock, Yang Xu, Zhenghua Su, Wentong Yang, Jamie Stuart Laird, Tian Zheng, Na Wu, Wusong Zha, Qun Luo, Chang-Qi Ma, Trevor A. Smith, Fangyang Liu, Paul Mulvaney
Summary: Photovoltaic thin film solar cells based on CZTSSe have limited efficiency due to the deficit in open circuit voltage. The proposed ZAZA window structure offers improved optoelectronic properties and carrier collection efficiency, resulting in a significant enhancement of VOC. These advances enable the fabrication of high-efficiency CZTSSe solar cells without the need for an anti-reflective coating.
Article
Nanoscience & Nanotechnology
Nikolina Kalcec, Nikolina Peranic, Ivan Mamic, Maja Beus, Christopher R. Hall, Trevor A. Smith, Marc Antoine Sani, Petra Turcic, Frances Separovic, Ivana Vinkovoic Vrcek
Summary: This study aims to improve the delivery of drugs for Parkinson's disease treatment by designing two different types of selenium nanoparticles (SeNPs). The results show that PVP-SeNPs have strong binding capacity with L-DOPA and dopamine. Additionally, the formation of protein corona plays a crucial role in the design of drug nanodelivery systems.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Mengjie Liu, Qingyang Li, Carlie Delaine, Hongkang Wu, Yanni Arsenakis, Barbara F. White, Briony E. Forbes, Chaitra Chandrashekar, Mohammed Akhter Hossain
Summary: Commercially available insulins for diabetes treatment are obtained through recombinant methods. By replacing the LysB29 residue in insulin with the CysB29 residue, we chemically synthesized a thiol-insulin scaffold (CysB29-insulin II) and conjugated it with palmitic acid using a highly efficient and selective thiol-maleimide reaction. The resulting palmitoyl-insulin, which is structurally similar to insulin, exhibits slow and long-acting properties in vitro and in vivo without significant byproduct formation.
Article
Chemistry, Multidisciplinary
Bruce Lin, Andrew Hung, William Singleton, Kevion K. Darmawan, Rachael Moses, Bicheng Yao, Hongkang Wu, Anders Barlow, Sani Marc-Antoine, Alastair J. Sloan, Mohammed Akhter Hossain, John D. Wade, Yuning Hong, Neil M. O'Brien-Simpson, Wenyi Li
Summary: Antimicrobial peptides (AMPs) are potential alternatives to antibiotics, and their attachment to lipid carbon chains can enhance their functionality in membrane interactions and antimicrobial activity. This study investigates the antimicrobial activity, oligomerization propensity, and lipid-membrane binding interactions of N-terminal lipidated analogs of MSI-78 (4-20) and pardaxin (1-22). Molecular modeling suggests that lipidation may confer greater structural stability and depth of membrane insertion to the AMPs. Experimental and computational findings shed light on how lipidation alters the conformation and functional properties of AMPs.
Article
Chemistry, Organic
Joses G. G. Nathanael, Bing Yuan, Christopher R. R. Hall, Trevor A. A. Smith, Uta Wille
Summary: Absolute second-order rate coefficients for the reaction of N- and C-protected amino acids with triethylamine-derived aliphatic peroxyl radical TEAOO were determined using laser flash photolysis. The reactions proceeded via radical hydrogen atom transfer (HAT) from the C alpha position, and the rate was determined by the exothermicity of the reaction. The preferred pathway for Ac-Tyr-OMe was HAT from the phenolic OH group, which shut down when hydrogen bonding with an amine occurred.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
