期刊
BIOPHYSICAL JOURNAL
卷 100, 期 5, 页码 L28-L30出版社
CELL PRESS
DOI: 10.1016/j.bpj.2011.01.043
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类别
资金
- National Institutes of Health [GM030376]
The charge versus voltage relation of voltage-sensor domains shifts in the voltage axis depending on the initial voltage. Here we show that in nonconducting W434F Shaker K+ channels, a large portion of this charge-voltage shift is apparent due to a dramatic slowing of the deactivation gating currents, Ig(D) (with tau up to 80 ms), which develops with a time course of similar to 1.8 s. This slowing in Ig(D) adds up to the slowing due to pore opening and is absent in the presence of 4-aminopyridine, a compound that prevents the last gating step that leads to pore opening. A remaining 10-15 mV negative shift in the voltage dependence of both the kinetics and the charge movement persists independently of the depolarizing prepulse duration and remains in the presence of 4-aminopyridine, suggesting the existence of an intrinsic offset in the local electric field seen by activated channels. We propose a new (to our knowledge) kinetic model that accounts for these observations.
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