Article
Chemistry, Analytical
Ziwei Hu, Xia Zhou, Jun Duan, Xueqiang Wu, Jiamin Wu, Pengcheng Zhang, Wanzhen Liang, Jialiang Guo, Huaihong Cai, Pinghua Sun, Haibo Zhou, Zhengjin Jiang
Summary: A novel aptamer SERS biosensor for highly sensitive protein biomarker detection was developed, achieving a limit of detection of 10 fM for C-reactive protein. The biosensor exhibited excellent selectivity and specificity for CRP, and high accuracy in the detection of actual human serum samples, showing great promise for clinical diagnosis of diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Inyong Hwang, Mingyun Kim, Jaeyeon Yu, Jihye Lee, Jun-Hyuk Choi, Su A. Park, Won Seok Chang, Jongwon Lee, Joo-Yun Jung
Summary: SEIRA spectroscopy utilizes near-field effects in nanostructures for highly sensitive detection, with a 10 nm thick MA achieving a record-high reflection difference SEIRA signal in experiments. MAs can be mass-produced through nanoimprint lithography and dry etching, offering a promising technological pathway for sensing and detection applications.
Article
Chemistry, Analytical
Hemavathi Krishnan, Subash C. B. Gopinath, M. K. Md Arshad, H. I. Zulhaimi, Periasamy Anbu, Sreeramanan Subramaniam
Summary: This study developed a rapid and sensitive biomimetic biosensor for the early detection of bleeding disorders. By combining aptamer recognition mechanism with molecular imprinted polymer technology, the authors improved the sensor sensitivity. The aptamer embedded MIP sensor showed higher sensitivity compared to conventional aptasensors and demonstrated specificity for analogues.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Napatsakorn Kamkrua, Thitaphat Ngernsutivorakul, Saksorn Limwichean, Pitak Eiamchai, Chanonthorn Chananonnawathorn, Viyapol Pattanasetthakul, Raffaele Ricco, Kiattawee Choowongkomon, Mati Horprathum, Noppadon Nuntawong, Tanujjal Bora, Raju Botta
Summary: In this study, a SERS-based aptasensor platform was developed for the sensitive and selective detection of the herbicide paraquat (PQ) by modifying gold (Au) nanoparticle SERS substrates with a thiol-containing aptamer. The aptamer-modified SERS substrates showed improved sensitivity and selective detection of PQ, with a limit of detection estimated to be 0.10 ± 0.03 μM. The developed aptasensor platform demonstrated good stability and selectivity against interferences from other similar herbicides and insecticides in natural water samples.
ACS APPLIED NANO MATERIALS
(2023)
Review
Chemistry, Analytical
Beibei Lou, Yanfei Liu, Meilin Shi, Jun Chen, Ke Li, Yifu Tan, Liwei Chen, Yuwei Wu, Ting Wang, Xiaoqin Liu, Ting Jiang, Dongming Peng, Zhenbao Liu
Summary: Viruses pose a serious threat to human life and health. Accurate early diagnosis is crucial for controlling and treating virus infections. In this review, the application of aptasensors for virus detection in recent years is summarized. The common detection mechanisms and the use of aptamers are illustrated. The relationships between virus biomarkers, detection methods, and their performance are revealed. Furthermore, the challenges and future directions of aptasensors are discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Feiyang Wang, Na Na, Jin Ouyang
Summary: The catalytic performance of gold nanomaterials, specifically a particle-in-a-frame nanostructure (PIAF) with interior nanogaps, was studied in this work. The Au PIAF was found to accurately identify 7 proteins, 5 antioxidants, and 3 cell types through catalysing the TMB reaction.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Pablo C. Serrano, Gisele E. Nunes, Lindiomar B. Avila Jr, Carleane P. S. Reis, Aldo M. C. Gomes, Francoise T. Reis, Maria L. Sartorelli, Silvia Pedroso Melegari, William G. Matias, Ivan H. Bechtold
Summary: Saxitoxin, a harmful cyanotoxin, requires monitoring in water bodies. An electrochemical impedimetric biosensor using a specific aptamer was developed for its detection, showing high sensitivity and selectivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Maroua Hamami, Abdelmoneim Mars, Noureddine Raouafi
Summary: A new nanometric aptasensing platform was developed for detecting aflatoxin M1 in milk, showing good sensitivity, low LOD, and good metrological performances. The platform was applied to detect AFM1 in pasteurized cow milk with promising results.
MICROCHEMICAL JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Junhong Chen, Xiaoyan Zhuang, Jiyang Zheng, Ruofan Yang, Fei Wu, Aihui Zhang, Baishan Fang
Summary: Aptamers, as functional nucleic acids, offer advantages over antibodies in terms of stability and ease of modification, and an Aptamer-Based Cell-free Detection (ABCD) system combining aptamers with CRISPR-Cas12a showed promise in detecting target proteins with low abundance. This system demonstrated high specificity and signal amplification capability, paving the way for future advancements in portable detection devices.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Qian Wang, Ping Li, Hao-Ming Wen, Kai-Jie Hu, Zi-Yue Huang, Jing Chen
Summary: Hydrogen organic frameworks (HOFs) are porous crystalline materials with various applications, but HOFs-based electrochemical aptasensors have not been reported. In this study, an electrochemical aptasensor based on HOFs constructed by 1,3,6,8-tetrakis[p-benzoic acid]pyrene was successfully developed. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the electrochemical performance of the HOF-based aptasensor for sensitive and selective detection of oxytetracycline.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Engineering, Electrical & Electronic
Shimwe Dominique Niyonambaza, Anna Koudrina, Maria C. DeRosa, Mounir Boukadoum, Amine Miled, Elodie Boisselier
Summary: This article presents a new method for dopamine detection using ultrastable gold nanoparticles, modified with a dopamine-binding aptamer, to achieve sensitive and selective detection of different concentrations of dopamine without inducing nanoparticle aggregation. The method shows good linearity between dopamine concentration and nanoparticle plasmon shift, with the ability to filter out interfering molecules and permit nanoparticle reuse.
IEEE SENSORS JOURNAL
(2021)
Article
Food Science & Technology
Sa Dong, Qiuyun Shi, Kangli He, Jianwei Wu, Zixin Zhu, Jianguo Feng
Summary: Chlorpyrifos, an organophosphorus insecticide, can be quantitatively detected using a new method developed in this study. The method involves loading the signal molecule 4-ATP into nanoparticles and coating them with an aptamer for chlorpyrifos. The release of 4-ATP correlates with the concentration of chlorpyrifos, and a standard curve can be established for quantitative analysis. The method shows good performance in apple and tomato samples.
Article
Chemistry, Analytical
Michael R. Stoneman, Valerica Raicu
Summary: This study investigated the impact of the interaction between a GPCR and its agonist ligand on the dielectric properties of the plasma membrane in living yeast cells using radiofrequency dielectric spectroscopy. The results showed a significant increase in plasma membrane permittivity after exposure to the agonist ligand, while cells lacking the ability to express the specific receptor showed no change. Interestingly, the electrical properties of the cellular interior also underwent a large change after the addition of the agonist, regardless of Ste2 expression.
Article
Chemistry, Analytical
Saipriya Ramalingam, Christopher M. Collier, Ashutosh Singh
Summary: Antibiotics are widely used in veterinary science to promote animal health and feed efficiency, but concerns arise due to the potential residues in milk. Therefore, quick and reliable testing methods are essential to ensure the safety of the produced milk.
Review
Chemistry, Analytical
Joon-Ha Park, Yun-Sik Eom, Tae-Hyung Kim
Summary: In recent years, there has been an increasing demand for highly sensitive and selective biosensors for neurotransmitters due to advancements in science and technology. Real-time sensing plays a crucial role in preventing neurological and cardiovascular diseases effectively. This review summarizes the latest progress in aptamer-based biosensor technology, focusing on various biomaterials used to ensure optimal performance and high selectivity. Its aim is to further advance aptamer-based biosensor technology.