期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 407, 期 23, 页码 7221-7227出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8886-9
关键词
Lignin; UHPLC-MS; UPLC-MS; S/G ratio
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2008/58035-6, 2011/51949-5, 2013/21306-0, 2012/21395-0]
- Conselho Nacional de Pesquisa-Brazil (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/21395-0] Funding Source: FAPESP
A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 mu g/mL, respectively. In addition, the method combines reliability and a fast and direct quantification.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据