Article
Multidisciplinary Sciences
Liam P. Dow, Guido Gaietta, Yair Kaufman, Mark F. Swift, Moara Lemos, Kerry Lane, Matthew Hopcroft, Armel Bezault, Cecile Sauvanet, Niels Volkmann, Beth L. Pruitt, Dorit Hanein
Summary: The authors developed a micropatterning workflow that allows control of cell positioning and morphology. By characterizing the nanoscale details of protein micropatterns on electron microscopy grids, they were able to study the structural changes governing cell-cell signaling.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Karen G. Soans, Ana Patricia Ramos, Jaydeep Sidhaye, Abhijeet Krishna, Anastasia Solomatina, Karl B. Hoffmann, Raimund Schluessler, Jochen Guck, Ivo F. Sbalzarini, Carl D. Modes, Caren Norden
Summary: Cell migration is crucial for organismal development, but the influence of physical properties on migration phenomena in vivo remains unclear. In this study, the researchers used zebrafish optic cup formation to investigate the role of extracellular matrix (ECM) properties in cell migration. They found that rim cells migrate over an immobile ECM and require cryptic lamellipodia for movement. The topology of the ECM changes along the migration path and is accompanied by changes in cell-matrix interactions. Matrix porosity is linked to efficient migration.
Article
Nanoscience & Nanotechnology
Cong Wu, Chriss S. M. Chin, Qingyun Huang, Ho-Yin Chan, Xinge Yu, Vellaisamy A. L. Roy, Wen J. Li
Summary: By culturing muscle cells on substrates with nanogrooves and polymer-based materials, we can study their response to biophysical cues. The results demonstrate that nanotopography and substrate stiffness independently regulate myoblast proliferation and morphology, with nanotopographical cues having a stronger effect.
MICROSYSTEMS & NANOENGINEERING
(2021)
Review
Cell Biology
Danielle Vahala, Yu Suk Choi
Summary: Breast cancer is a significant burden, and metastasis poses a challenge for patient survival. Disease progression involves remodeling of the extracellular matrix (ECM). The stiffness of breast cancer tissue increases due to collagen production by cancer-associated fibroblasts (CAFs), which creates a favorable environment for tumor progression. Researchers have developed biomaterials to mimic in vivo tissue and explore how ECM remodeling and cell-ECM adhesions regulate tumor growth and cancer cell plasticity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Gabriel Beltran, Daniel Navajas, Jose Manuel Garcia-Aznar
Summary: The mechanical signals sensed by alveolar cells are determined by the local matrix stiffness of the extracellular matrix (ECM). This study developed a finite element (FE) model based on Voronoi tessellation to accurately reproduce the mechanical behavior of a decellularized rat lung tissue strip. The position of cells within the alveoli greatly influences the local stiffness perceived by the cells, with corner cells perceiving higher stiffness compared to flat area cells.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Shuyi Qian, Yixiao Dong, Ju Qu, Xuechun Wang, Wang Zhang, Jia Chen, Fan Xu, Meihua Cui, Monica Giomo, Chenhan Liao, Manli Hu, Juan Xu, Ganlu Hu, Jie Zheng, Xianmin Zhu, Anna Urciuolo, Guoping Fan, Nicola Elvassore
Summary: In this study, a facile strategy was proposed to produce precise channel-like hydrogel confinements on a microfluidic device for investigating cancer cell migration and mechanical interactions. The results demonstrated that breast cancer cells migrated faster in stiffer confinements with an adapted migratory phenotype, and the activation of YAP was involved in this process.
ACS MATERIALS LETTERS
(2023)
Article
Multidisciplinary Sciences
Celine Labouesse, Bao Xiu Tan, Chibeza C. Agley, Moritz Hofer, Alexander K. Winkel, Giuliano G. Stirparo, Hannah T. Stuart, Christophe M. Verstreken, Carla Mulas, William Mansfield, Paul Bertone, Kristian Franze, Jose C. R. Silva, Kevin J. Chalut
Summary: The study explores the independent control of substrate stiffness and extracellular matrix tethering by developing StemBond hydrogels. The hydrogels are validated to provide an optimal system for culturing mouse and human pluripotent stem cells, and they modulate stem cell function, partly through stiffness-sensitive ERK signaling. Ultimately, the findings emphasize the importance of optimizing the complete mechanical microenvironment for greater control over stem cell fate specification.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yongbum Cho, JangHyuk Kim, Jeehun Park, Junsang Doh
Summary: Natural killer (NK) cells are innate cytotoxic lymphocytes that target virally infected cells and tumor cells. In this study, the effects of surface topography and tumor cell morphology on NK cell cytotoxicity were investigated. The results suggest that the biophysical environments of tumor cells regulate NK cell cytotoxicity.
MATERIALS HORIZONS
(2023)
Article
Chemistry, Analytical
Guocheng Zhang, Yufang Chang, Na Fan, Bin Yan, Xianmeng Li, Zihan Yang, Zhenyang Yu
Summary: This study examines the relationship between cell stiffness and the insertion efficiency of atomic force microscope (AFM) tips into cell membranes. The results show that cell stiffness is determined by the prestress of the cell membrane, and higher stiffness leads to greater insertion efficiency. Finite element analysis confirms that prestress increases the bending stiffness of the cytoskeleton, enabling better support for the cell membrane and resulting in stress concentration at the contact area between AFM tips and cell membranes. Increasing prestress of cell membranes could significantly improve insertion efficiency.
Article
Multidisciplinary Sciences
Xunwu Hu, Sona Rani Roy, Chengzhi Jin, Guanying Li, Qizheng Zhang, Natsuko Asano, Shunsuke Asahina, Tomoko Kajiwara, Atsushi Takahara, Bolu Feng, Kazuhiro Aoki, Chenjie Xu, Ye Zhang
Summary: Engineering peptide assembly that controls integrin ligand presentation on the molecular level is of great significance, expanding the perspective of ligand-density-dependent modulation and providing valuable insights for therapeutic innovations. The bottom-up nanofabrication strategy allows for precise control of ligand presentation and the production of multifunctional nanofilaments.
NATURE COMMUNICATIONS
(2022)
Review
Physiology
Natalia A. Osna, Akiko Eguchi, Ariel E. Feldstein, Hidekazu Tsukamoto, Raghubendra S. Dagur, Murali Ganesan, Moses New-Aaron, Madan Kumar Arumugam, Srinivas Chava, Marcelle Ribeiro, Gyongyi Szabo, Sebastian Mueller, Shijin Wang, Cheng Chen, Steven A. Weinman, Kusum K. Kharbanda
Summary: This review focuses on the communication between liver parenchymal and non-parenchymal cells induced by alcohol, which plays a significant role in the development of liver injury. Various mechanisms are involved in the information exchange between different cell types, and this exchange may promote the progression of end-stage liver disease. The review highlights the activation of non-parenchymal cells, liver macrophages, and hepatic stellate cells by extracellular vesicles derived from alcohol-exposed hepatocytes. Additionally, it discusses the crosstalk between cell death pathways and inflammasome activation in alcohol-activated cells, as well as the role of non-inflammatory factors, sinusoidal pressure, and hepatic arterialization in alcohol-induced hepatic fibrogenesis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physics, Multidisciplinary
Macia Esteve Pallares, Irina Pi-Jauma, Isabela Corina Fortunato, Valeria Grazu, Manuel Gomez-Gonzalez, Pere Roca-Cusachs, Jesus M. de la Fuente, Ricard Alert, Raimon Sunyer, Jaume Casademunt, Xavier Trepat
Summary: The migratation of cellular clusters is important for various biological processes, but the mechanisms underlying collective durotaxis are not fully understood. This study reveals a connection between collective durotaxis and the wetting properties of cellular clusters. The findings show that the wetting behavior of cancer cells on substrates is dependent on the stiffness of the substrates. The study also demonstrates a physical mechanism of collective durotaxis based on the wetting properties of active droplets.
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Namrata Ramani, C. Adrian Figg, Alex J. Anderson, Peter H. Winegar, EunBi Oh, Sasha B. Ebrahimi, Devleena Samanta, Chad A. Mirkin
Summary: A method to pattern multiple oligonucleotide sequences in hydrogels using thiol-yne photochemistry is introduced, enabling the study of cell behavior and signaling in complex spatial environments.
ADVANCED MATERIALS
(2023)
Review
Cell Biology
Andrea Ravasio, Eugenia Morselli, Cristina Bertocchi
Summary: Cells respond to mechanical forces and physical cues from their environment, with autophagy playing a major role in maintaining cellular processes and regulating interactions with the environment. This review summarizes the interaction between autophagy and mechanotransduction machinery associated with cell adhesions, termed as "Mechanoautophagy".
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Letter
Anesthesiology
Claude Guerin, Martin Cour, Florian Degivry, Francois Charbon, Bruno Louis, Laurent Argaud, Nicolas Terzi
EUROPEAN JOURNAL OF ANAESTHESIOLOGY
(2022)
Article
Biophysics
Marion Tardieu, Najat Salameh, Line Souris, David Rousseau, Laurene Jourdain, Hanadi Skeif, Francois Prevot, Ludovic de Rochefort, Denis Ducreux, Bruno Louis, Philippe Garteiser, Ralph Sinkus, Luc Darrasse, Marie Poirier-Quinot, Xavier Maitre
Summary: Magnetic resonance elastography is a non-invasive and remote technique to characterize the mechanical properties of living tissues. By guiding pressure waves through the buccal cavity and bypassing the skull, quantitative measurement of mechanical properties in the human brain can be obtained.
NMR IN BIOMEDICINE
(2022)
Editorial Material
Otorhinolaryngology
Margaux Petitjean, Emilie Bequignon, Maxime Fieux, Bruno Louis, Francoise Zerah, Andre Coste, Sophie Bartier
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2022)
Article
Cell Biology
Alexander Kyumurkov, Anne-Pascale Bouin, Mathieu Boissan, Sandra Manet, Francesco Baschieri, Mathilde Proponnet-Guerault, Martial Balland, Olivier Destaing, Myriam Regent-Kloeckner, Claire Calmel, Alice Nicolas, Francois Waharte, Philippe Chavrier, Guillaume Montagnac, Emmanuelle Planus, Corinne Albiges-Rizo
Summary: ICAP-1 regulates integrin endocytosis by interacting with NME2, a key regulator of dynamin-dependent clathrin-coated pits fission. The control of clathrin-mediated integrin endocytosis by an integrin inhibitor is a unique mechanism to finely tune physical forces at focal adhesions. The impact of internalization on cellular mechanics and its mechanism remains elusive.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Pediatrics
Claude Danan, Manon Tauzin, Camille Jung, Xavier Durrmeyer, Laurence Caeymaex, Charles Treussart, Fabrice Decobert, Bruno Louis
Summary: The humidity during HFOV was significantly lower than during IPPV, both in vitro and in vivo. The type of heater-humidifier also influenced humidification. More systematic measurements of humidity of inspired gas, especially during HFOV, should be considered to optimize humidification and consequently lung protection in ELBW infants.
PEDIATRIC PULMONOLOGY
(2023)
Article
Critical Care Medicine
Mathieu Delorme, Karl Leroux, Antoine Leotard, Ghilas Boussaid, Helene Prigent, Bruno Louis, Frederic Lofaso
Summary: A study found that current noninvasive ventilation devices have varied responses and scoring of sleep-related respiratory events. Further improvements in algorithms are needed to enable more widespread initiation and follow-up of domiciliary noninvasive ventilation.
Article
Medicine, General & Internal
Martin Cour, Claude Guerin, Florian Degivry, Laurent Argaud, Bruno Louis
Summary: This study compared the work of breathing (WOB) using a tracheal cannula (TC) and a nasal cannula (NC) during high flow oxygen therapy (HFOT), and found that the WOB was lower with the TC than with the NC. However, these differences were very small and may not be clinically relevant.
FRONTIERS IN MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Florent Carsuzaa, Emilie Bequignon, Sophie Bartier, Andre Coste, Xavier Dufour, Matthieu Bainaud, Jean Claude Lecron, Bruno Louis, Stephane Tringali, Laure Favot, Maxime Fieux
Summary: This study investigated the presence of OSM and IL-6, as well as the expression pattern of tight junctions, in CRSwNP patients and controls. The results showed that OSM and IL-6 were overexpressed, and tight junction (ZO-1 and occludin) expression was decreased in nasal polyps compared to control mucosa. In vitro experiments further demonstrated that OSM could significantly reduce tight junction expression, transepithelial electric resistance, and ciliary beating efficiency, while promoting cell repair mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Amene Majidipur, Margot Morin-Dewaele, Jeanne Gaspar Lopes, Francois Berry, Julien Fouchet, Sophie Bartier, Anais Dufros Duval, Pascale Soyeux, Eric Huet, Bruno Louis, Andre Coste, Emilie Bequignon, Carolina Saldana, Philippe Le Corvoisier, Damien Destouches, Jean-Michel Pawlotsky, Alexandre de la Taille, Francis Vacherot, Patrice Bruscella, Virginie Firlej
Summary: In early 2020, the emergence of SARS-CoV-2 in Wuhan, China caused a global health emergency. The virus enters cells through the ACE2 protein and TMPRSS2 facilitates membrane fusion. TMPRSS2 is regulated by AR signaling, and inhibiting it with apalutamide reduces SARS-CoV-2 entry and infection in lung and nasal cells. These findings suggest that apalutamide could be a treatment option for PCa patients at risk for severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Critical Care Medicine
Nicolas Terzi, Isabelle Vaugier, Claude Guerin, Helene Prigent, Ghilas Boussaid, Karl Leroux, Mathieu Delorme, Frederic Lofaso, Bruno Louis
Summary: This study compares the measurement of CPF and ECV between 4 MI-E devices under simulated conditions. The results show significant differences in CPF values and ECV values among different devices, suggesting limitations in using only CPF values to determine cough effectiveness.
Article
Multidisciplinary Sciences
Adrien Mery, Artur Ruppel, Jean Revilloud, Martial Balland, Giovanni Cappello, Thomas Boudou
Summary: The authors engineer 3D microtissues from optogenetically modified fibroblasts and utilize light to induce local contractions and measure tissue stress and strain. They investigate the regulation and spatio-temporal distribution of these contractions, as well as the potential of light to create and map local anisotropies in mechanically heterogeneous microtissues. This study offers a new approach to guide tissue formation and non-destructively chart their rheology in real time using their own constituent cells as internal actuators.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Nioosha Nekooie Marnany, Redouane Fodil, Sophie Fereol, Alwyn Dady, Marine Depp, Frederic Relaix, Roberto Motterlini, Roberta Foresti, Jean-Loup Duband, Sylvie Dufour
Summary: Glucose metabolism plays a crucial role in the development of avian trunk neural crest cells (NCCs). Trunk NCCs prefer glucose oxidation, while cranial NCCs rely on aerobic glycolysis. Multiple pathways, including glycolysis, mitochondrial respiration, and the pentose phosphate pathway, are mobilized and integrated for the coordinated execution of diverse cellular programs in trunk NCCs.
JOURNAL OF CELL SCIENCE
(2023)
Article
Medicine, General & Internal
Noemie Bricmont, Romane Bonhiver, Lionel Benchimol, Bruno Louis, Jean-Francois Papon, Justine Monseur, Anne-Francoise Donneau, Catherine Moermans, Florence Schleich, Doriane Calmes, Anne-Lise Poirrier, Renaud Louis, Marie-Christine Seghaye, Celine Kempeneers
Summary: In this study, we evaluated the effects of delay after sampling and the temperature for conservation of respiratory ciliated samples on assessments of ciliary beating. We found that ciliary beating can be evaluated at 37 degrees Celsius up to 9 hours after nasal brushing, but the storage temperature can modify ciliary beating.
Article
Critical Care Medicine
Anne-Fleur Haudebourg, Elsa Moncomble, Arnaud Lesimple, Flora Delamaire, Bruno Louis, Armand Mekontso Dessap, Alain Mercat, Jean-Christophe Richard, Francois Beloncle, Guillaume Carteaux
Summary: This study proposes a novel method for assessing airway opening pressure (AOP) during volume assist control ventilation at a constant-flow rate of 60 L/min. The P-cond method, which compares the airway pressure waveform to detect and measure AOP, was validated and showed good respiratory and hemodynamic tolerance compared to the standard low-flow method.