Article
Chemistry, Analytical
Emmanuel Berlin, Veronica Lizano-Fallas, Ana Carrasco del Amor, Olatz Fresnedo, Susana Cristobal
Summary: Membrane proteins are important for understanding cellular function, but the unbiased identification of these proteins is challenging. The use of nonionic surfactants to enrich the soluble proteome can affect protein solubility and thermal stability. The introduction of nonionic surfactants is not compatible with target identification by thermal proteome profiling methodologies. Finding surfactant-free strategies for identifying membrane protein targets is the next challenge.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Luis M. Real Hernandez, Ilya Levental
Summary: Synthetic copolymers can form nanodiscs by directly intercalating into intact membranes, but the nanodiscs do not completely retain the physical properties of intact membranes.
BIOPHYSICAL JOURNAL
(2023)
Article
Biophysics
Samantha T. Clark, Matthias M. L. Arras, Stephen A. Sarles, Paul D. Frymier
Summary: This study investigates the association of a small molecule with two different membrane types by modeling and experimentally observing the association of detergent Triton X-100 with egg phosphatidylcholine (ePC) liposomes and egg phosphatidic acid (ePA) liposomes at varying ratios. A saturation model was developed to predict the association of detergent with each liposome type when lipid bilayers are fully saturated, based on the detergent to lipid ratio and relative absorbance intensity for each pure liposome species at saturation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Curran G. Gahan, Reid C. Van Lehn, Helen E. Blackwell, David M. Lynn
Summary: This study found that the length and oxidation state of AHL acyl tail groups influence their interactions with model lipid membranes, with the presence of a 3-oxo group impacting membrane remodeling. AHLs with 3-oxo groups generally promote microtubule formation, while AHLs without 3-oxo groups tend to form hemispherical caps on lipid bilayers. Additionally, 3-oxo AHLs showed greater association with lipid bilayers and facilitated greater transport or leakage of cargo molecules across membranes compared to non-3-oxo AHLs, indicating increased bilayer disruption and destabilization.
Article
Biochemistry & Molecular Biology
Oleg Batishchev, Natalia Kuzmina, Andrey A. Mozhaev, Alexander S. Goryashchenko, Ekaterina D. Mileshina, Alexander N. Orsa, Eduard Bocharov, Igor E. Deyev, Alexander G. Petrenko
Summary: This study provides insights into the structural and activation mechanisms of IRR, identifying two conformations of inactive IRR in neutral media and two presumably active IRR conformations at pH 9.0, all showing formation of intracellular catalytic domains responsible for autophosphorylation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Rafael B. Lira, Fernanda S. C. Leomil, Renan J. Melo, Karin A. Riske, Rumiana Dimova
Summary: This study investigates the impact of membrane surface charge and medium composition on membrane stability, explores the destabilization responses of charged lipid vesicles under strong electric pulses, and identifies the role of membrane edge tension in pore closure. The findings are crucial for understanding mechanisms in cells to prevent total membrane collapse upon poration.
Review
Biochemistry & Molecular Biology
Johanna Detzner, Gottfried Pohlentz, Johannes Muthing
Summary: This review discusses the clinical impact of Enterohemorrhagic Escherichia coli (EHEC) infections, focusing on the vesicular packaging of Shiga toxin (Stx) in the intestine and blood stream, as well as the extraintestinal complications and therapeutic options. The role of epithelial cells of the colon and kidneys in the infection process is also addressed. The review highlights the importance of Stx-binding glycosphingolipids (Gb3Cer and Gb4Cer) and their distribution in primary human kidney and colon epithelial cells. The susceptibility of primary renal and colonic epithelial cells to Stx is discussed, suggesting a resilience of the intestinal epithelium against the human-pathogenic Stx subtypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Andressa R. Pereira, Flavio M. Shimizu, Osvaldo N. Oliveira Jr
Summary: The composition of Langmuir monolayers, specifically the relative concentration of cholesterol, plays a crucial role in modulating the effects of the antineoplastic drug paclitaxel. The dependence on cholesterol concentration for these monolayers simulating lipid rafts is non-monotonic, affecting surface pressure and compressibility modulus differently. The physiological action of paclitaxel may vary depending on its interaction with membrane penetration or membrane elasticity changes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Engineering, Chemical
John Chau, Kamalesh K. Sirkar, Kenneth J. Pennisi, Gazelle Vaseghi, Lotfi Derdour, Benjamin Cohen
Summary: Polymeric membranes for organic solvent nanofiltration in pharmaceutical applications need to be highly resistant to various organic solvents. By studying perfluoropolymers, it is possible to enhance the flux of polar aprotic solvents. Experimental results showed that these membranes can achieve high solute rejections up to 95% under certain conditions.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Physical
Jordan A. Doumanov, Kirilka Mladenova, Vesselina Moskova-Doumanova, Tonya D. Andreeva, Svetla D. Petrova
Summary: This article reviews the current understanding of hBest1 surface organization, interactions with membrane lipids in model membranes, and its association with microdomains of cellular membranes, which are important for modulation of channel activity in cells.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biology
Nipuni Mayadunna, Samantha C. Karunarathna, Suhail Asad, Steven L. Stephenson, Abdallah M. Elgorban, Salim Al-Rejaie, Jaturong Kumla, Neelamanie Yapa, Nakarin Suwannarach
Summary: The study aimed to evaluate the phosphate-solubilizing efficiency of phosphate-solubilizing microorganisms (PSMs) using tricalcium phosphate (TCP) and Eppawala rock phosphate (ERP). A series of identified isolates were compared in phosphate solubilization using TCP and ERP as a P source. Twelve isolates with higher efficiency were selected for biofertilizer production. The pot trial with Capsicum annuum L. showed that the application of native PSMs as biofertilizer effectively increased plant height, leaf length, and width.
Review
Cell Biology
Leticia Labat-de-Hoz, Armando Rubio-Ramos, Javier Casares-Arias, Miguel Bernabe-Rubio, Isabel Correas, Miguel A. Alonso
Summary: Primary cilia are microtubule-based protrusions surrounded by a ciliary membrane equipped with selected receptors that control important signaling pathways in cell growth and development. This review presents a model of primary ciliogenesis by an alternative route involving the midbody remnant from cytokinesis, which delivers membranes to the centrosome for ciliary membrane assembly. The involvement of the midbody remnant in primary cilium formation and its regulation by the ESCRT machinery are discussed, along with the emerging roles of membrane compartmentalization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ilya O. Aparin, Rui Yan, Remi Pelletier, Alexander A. Choi, Dmytro I. Danylchuk, Ke Xu, Andrey S. Klymchenko
Summary: This study proposes a concept of ON/OFF switchable probes for super-resolution fluorescence microscopy based on bright cyanine dyes. The new probes provide higher brightness and slower diffusion at the cell surface, improving the localization precision. The study validated the concept and demonstrated the observation of nanoscale cellular structures with enhanced dynamics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Kerrie A. Morrison, Laura Wood, Karen J. Edler, James Doutch, Gareth J. Price, Francoise Koumanov, Paul Whitley
Summary: Extraction of integral membrane proteins using poly(styrene-co-maleic acid) can retain the lipid bilayer surrounding the proteins. This study demonstrates efficient extraction of the mammalian insulin receptor with the copolymer but reveals a lack of functional response to insulin after extraction.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Bartholomaus Danielczak, Marie Rasche, Julia Lenz, Eugenio Perez Patallo, Sophie Weyrauch, Florian Mahler, Michael Tope Agbadaola, Annette Meister, Jonathan Oyebamiji Babalola, Carolyn Vargas, Cenek Kolar, Sandro Keller
Summary: Glyco-DIBMA, a novel glycopolymer, demonstrates enhanced efficiency in solubilizing lipid vesicles to form nanodiscs under near-physiological conditions, maintaining a bilayer structure and enabling rapid lipid exchange. Experimental results have shown the superior performance of Glyco-DIBMA in extracting cellular membrane proteins and analytical applications.
Correction
Multidisciplinary Sciences
Sreemoti Banerjee, Robert J. Andrew, Christopher J. Duff, Kate Fisher, Carolyn D. Jackson, Catherine B. Lawrence, Nobuyo Maeda, Daniel S. Greenspan, Katherine A. B. Kellett, Nigel M. Hooper
SCIENTIFIC REPORTS
(2020)
Article
Neurosciences
Barbara Kramarz, Rachael P. Huntley, Milagros Rodriguez-Lopez, Paola Roncaglia, Shirin C. C. Saverimuttu, Helen Parkinson, Rina Bandopadhyay, Maria-Jesus Martin, Sandra Orchard, Nigel M. Hooper, David Brough, Ruth C. Lovering
JOURNAL OF ALZHEIMERS DISEASE
(2020)
Article
Biochemistry & Molecular Biology
David A. Hicks, Alys C. Jones, Nicola J. Corbett, Kate Fisher, Stuart M. Pickering-Brown, Mark P. Ashe, Nigel M. Hooper
NEUROCHEMICAL RESEARCH
(2020)
Article
Polymer Science
Samuel R. Moxon, Miguel J. S. Ferreira, Patricia dos Santos, Bogdan Popa, Antonio Gloria, Ramaz Katsarava, David Tugushi, Armenio C. Serra, Nigel M. Hooper, Susan J. Kimber, Ana C. Fonseca, Marco A. N. Domingos
Review
Medicine, Research & Experimental
Nigel M. Hooper, Daniel W. Lambert, Anthony J. Turner
Article
Geriatrics & Gerontology
Ross A. Dunne, Dag Aarsland, John T. O'Brien, Clive Ballard, Sube Banerjee, Nick C. Fox, Jeremy D. Isaacs, Benjamin R. Underwood, Richard J. Perry, Dennis Chan, Tom Dening, Alan J. Thomas, Jeffrey Schryer, Anne-Marie Jones, Alison R. Evans, Charles Alessi, Elizabeth J. Coulthard, James Pickett, Peter Elton, Roy W. Jones, Susan Mitchell, Nigel Hooper, Chris Kalafatis, Jill G. C. Rasmussen, Helen Martin, Jonathan M. Schott, Alistair Burns
Summary: MCI is a clinically heterogeneous syndrome that can lead to further cognitive decline, but may also remain stable or resolve over time. Diagnostic criteria, including cognitive testing, neuroimaging, and fluid biomarkers, can improve accuracy of diagnosis and potentially guide prognosis. Large-scale studies are needed to determine the practical usefulness of combining multiple markers for clinical use.
Article
Geriatrics & Gerontology
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Stefano Patassini, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper
Summary: The study found alterations in levels of several metals across multiple regions of Parkinson's disease dementia (PDD) brain, with widespread decreases in copper being the most common, closely resembling those in Alzheimer's disease dementia (ADD) patients, suggesting similar disease mechanisms in both dementias.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Marilena Hadjidemetriou, Jack Rivers-Auty, Lana Papafilippou, James Eales, Katherine A. B. Kellett, Nigel M. Hooper, Catherine B. Lawrence, Kostas Kostarelos
Summary: Blood-circulating biomarkers have the potential to detect AD pathology early and improve clinical trial outcomes, but currently there are no validated blood-based biomarkers for AD. A nanoparticle-based proteomics enrichment approach was used to discover and track changes in the blood proteome in an AD mouse model, revealing multiple proteomic signals indicative of asymptomatic stages of AD. The potential of this nano-omics approach to enrich the identification of blood-circulating signals associated with neurodegeneration was demonstrated, emphasizing the importance of integrating nanotechnology with proteomic tools for predicting the progression of AD.
Article
Neurosciences
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Stefano Patassini, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper
Summary: Elevated brain urea levels have been reported in age-related dementias such as Alzheimer's disease and Huntington's disease. This study found substantively elevated urea concentrations in all nine regions of the brain in Parkinson's disease dementia, indicating a common metabolic disturbance shared with Alzheimer's disease and Huntington's disease.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Katherine A. B. Kellett, Kate Fisher, Harry Aldworth, Nigel M. M. Hooper
Summary: Bone morphogenetic protein 1 (BMP1), a member of the astacin family of zinc-metalloproteases, cleaves LDL receptor (LDLR) within its ligand-binding domain, reducing LDL-cholesterol binding and uptake. Only BMP1, among the other astacin proteases, was found to contribute to the cleavage of LDLR. Mutations at the P1' and P2 positions of the cleavage site rendered mouse LDLR susceptible to BMP1 cleavage. This study provides insight into the mechanisms regulating LDLR function.
Article
Neurosciences
Wenjun Zhang, Xiangjun Zhao, Xuewei Qi, Susan J. Kimber, Nigel M. Hooper, Tao Wang
Summary: In this study, induced pluripotent stem cell (iPSC) models were established from CADASIL patients to investigate the abnormal neurovascular interaction and blood-brain barrier (BBB) function. The impaired capability of vascular mural cells in CADASIL patients and the decreased barrier function of blood-brain barrier endothelial cells were observed. These findings provide new insights into early disease pathologies and have implications for future therapeutic development.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Rheumatology
A. M. Snedden, K. A. B. Kellett, J. B. Lilleker, N. M. Hooper, H. Chinoy
Summary: This review discusses the pathological effects of protein aggregation and the process of aggregation following immune attack in inclusion body myositis (IBM). Immune attack is likely to cause protein aggregation by impairing endoplasmic reticulum and mitochondrial function. Aggregated proteins may activate inflammatory responses or provoke immune reactions. Protein aggregates may impair cellular mechanisms and propagate along myocytes, resulting in muscle weakness and atrophy.
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
D. Merryweather, S. R. Moxon, A. J. Capel, N. M. Hooper, M. P. Lewis, P. Roach
Summary: Cellular metabolism and behaviour are closely linked to cytoskeletal tension and scaffold mechanics. In this study, a 3D culture system using diluted type I collagen hydrogel to produce variation in gel stiffness was explored. The results showed that hydrogel scaffold remodelling was linked to gel collagen concentration and different concentrations of gel led to significant changes in programmed cell behaviour and cell morphology.
Article
Biochemistry & Molecular Biology
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Andrew C. Robinson, Natalie J. Gardiner, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper
Article
Biochemistry & Molecular Biology
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Sarah Kassab, Natalie J. Gardiner, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper