期刊
BIOPHYSICAL JOURNAL
卷 95, 期 4, 页码 1627-1638出版社
CELL PRESS
DOI: 10.1529/biophysj.108.130096
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资金
- NCRR NIH HHS [P41 RR013186, P41 RR13186] Funding Source: Medline
- NIGMS NIH HHS [U54 GM64346, U54 GM064346] Funding Source: Medline
Cell migration is based on an actin treadmill, which in turn depends on recycling of G-actin across the cell, from the rear where F-actin disassembles, to the front, where F-actin polymerizes. To analyze the rates of the actin transport, we used the Virtual Cell software to solve the diffusion-drift-reaction equations for the G-actin concentration in a realistic three-dimensional geometry of the motile cell. Numerical solutions demonstrate that F-actin disassembly at the cell rear and assembly at the front, along with diffusion, establish a G-actin gradient that transports G-actin forward globally'' across the lamellipod. Alternatively, if the F-actin assembly and disassembly are distributed throughout the lamellipod, F-/G-actin turnover is local, and diffusion plays little role. Chemical reactions and/or convective flow of cytoplasm of plausible magnitude affect the transport very little. Spatial distribution of G-actin is smooth and not sensitive to F-actin density fluctuations. Finally, we conclude that the cell body volume slows characteristic diffusion-related relaxation time in motile cell from similar to 10 to similar to 100 s. We discuss biological implications of the local and global regimes of the G-actin transport.
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