Article
Multidisciplinary Sciences
So-Jin Park-Holohan, Elisabetta Brunello, Thomas Kampourakis, Martin Rees, Malcolm Irving, Luca Fusi
Summary: The regulation of myosin activity in heart muscle involves the modulation of myosin motors available for interaction with thin filaments. Stretch or increased RLC phosphorylation do not have a direct effect on the activation of folded myosin motors at physiological conditions, but can lead to delayed activation and increased force production at higher calcium concentrations. This suggests a calcium-dependent interfilament signaling pathway involving thin filament sensitization and thick filament mechanosensing in the increased contractility induced by RLC phosphorylation and stretch in the heart.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Ashley A. Martin, Brian R. Thompson, Dongwoo Hahn, Addeli Bez Batti Angulski, Nora Hosny, Houda Cohen, Joseph M. Metzger
Summary: This review emphasizes the signaling components and regulatory mechanisms that impact cardiac sarcomere function, with a focus on the roles of the thick and thin filaments. The emerging field of inter-myofilament signaling and its important mediators are also discussed. Additionally, recent methods for studying the sarcomere under intact, physiologically relevant conditions are reviewed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Shengjie Sun, Chitra Karki, Bruce Z. Gao, Lin Li
Summary: This study reveals the importance of the binding and dissociation processes in the kinetic cycle of cardiac myosin, providing a new direction for future genetic disease studies.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Cardiac & Cardiovascular Systems
Nikola Kozhuharov, Desiree Wussler, Thomas Kaier, Ivo Strebel, Samyut Shrestha, Dayana Flores, Albina Nowak, Zaid Sabti, Thomas Nestelberger, Tobias Zimmermann, Joan Walter, Maria Belkin, Eleni Michou, Pedro Lopez Ayala, Danielle M. Gualandro, Dagmar Keller, Assen Goudev, Tobias Breidthardt, Christian Mueller, Michael Marber
Summary: In a study of patients with acute dyspnea in the emergency department, cardiac myosin-binding protein C (cMyC) showed diagnostic and prognostic value in relation to acute heart failure (AHF), with diagnostic accuracy higher than high-sensitivity cardiac troponin T (hs-cTnT) and prognostic accuracy comparable to NT-proBNP.
EUROPEAN JOURNAL OF HEART FAILURE
(2021)
Review
Cardiac & Cardiovascular Systems
Emna Riguene, Maria Theodoridou, Laila Barrak, Mohamed A. Elrayess, Michail Nomikos
Summary: Athletic performance is influenced by environmental and genetic factors. Tools like molecular methods, personalized training, and dietary requirements can help improve performance. Investigating genetic markers and phenotypes is crucial for monitoring elite endurance. Metabolomics can provide insights into physiological states and exercise-related disorders. SNPs in the MYBPC3 gene have been found to be associated with endurance athlete status.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Roua Hassoun, Constanze Erdmann, Sebastian Schmitt, Setsuko Fujita-Becker, Andreas Muegge, Rasmus R. Schroeder, Matthias Geyer, Mina Borbor, Kornelia Jaquet, Nazha Hamdani, Hans Georg Mannherz
Summary: Expression of cardiac alpha-actin and its mutants using the insect cell system revealed differences in filament length and morphology, as well as varying Ca2+-sensitivity of myosin-S1 ATPase stimulation. The addition of cardiac tropomyosin and troponin affected the enzyme activity differently in wild type and mutant actins. Overall, hypertrophic and dilated cardiomyopathy-related mutants showed distinct responses to Ca2+-sensitivity compared to the wild type.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Bertrand C. W. Tanner, Michael J. Previs, Yuan Wang, Jeffrey Robbins, Bradley M. Palmer
Summary: The thick filament protein cMyBP-C influences sarcomere stiffness and modulates cardiac contraction-relaxation through its phosphorylation. Studies have shown that phosphorylation or ablation of cMyBP-C can increase the rate of MgADP release and accelerate myosin detachment. Additionally, cMyBP-C inhibits Pi-dependent reversal of the power stroke, and its phosphorylation or absence can release this inhibition, speeding up myosin detachment.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Biology
Matthew H. Doran, William Lehman
Summary: Actin, a highly conserved protein, plays diverse roles in cellular processes by interacting with actin-binding proteins. The ATP-dependent cycle of myosin attachment and detachment drives muscle contraction and cellular transport. The variations in actin function are influenced by myosin isoforms and other actin-binding proteins.
Article
Physiology
Andrielle E. Capote, Ashley Batra, Chad M. Warren, Shamim A. K. Chowdhury, Beata M. Wolska, R. John Solaro, Paola C. Rosas
Summary: Experiments in this study looked at the role of beta-arrestin-2 in preserving cardiac function during aging, finding that beta-arrestin-2 signaling may play a significant role in protecting cardiac function in aging.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Niclas Olsson, Wei Jiang, Lital N. Adler, Elizabeth D. Mellins, Joshua E. Elias
Summary: This study investigates the effects of DO:DM ratios on peptide presentation by creating a panel of HLA-DR4-expressing APC lines and analyzing over 10,000 unique DR4-presented peptides. The findings provide insights into the characteristics that influence peptide presentation and have implications for HLA-II prediction algorithms and research strategies.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Pietro Ridone, Daniel L. Winter, Matthew A. B. Baker
Summary: This study focused on the role of the central MotB dimer in the bacterial flagellar motor and used modeling and energy calculations to identify stable states. The findings provide insights into the stability hierarchy and coordinated rotation of the MotB dimer.
Article
Medical Laboratory Technology
Bashir Alaour, Torbjorn Omland, Janniche Torsvik, Thomas E. Kaier, Marit S. Sylte, Heidi Strand, Jasmine Quraishi, Sam McGrath, Luke Williams, Steven Meex, Simon Redwood, Michael Marber, Kristin M. Aakre
Summary: Research on weekly biological variation of cardiac myosin-binding protein C (cMyC) showed that it is suitable for disease monitoring, risk stratification, and prognostication, with analytical quality specifications comparable to cardiac troponin.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Cell Biology
Khairun Nisaa, Anat Ben-Zvi
Summary: Muscle proteostasis is regulated by the myogenic transcription factor MyoD. This study demonstrates that MyoD can modulate chaperone expression in terminally differentiated muscle cells, indicating its role in muscle maintenance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Cao Wang, Shupei Qiao, Yufang Zhao, Hui Tian, Wei Yan, Xiaolu Hou, Ruiqi Wang, Bosong Zhang, Chaofan Yang, Fuxing Zhu, Yanwen Jiao, Jiaming Jin, Yue Chen, Weiming Tian
Summary: The main characteristic of myocardial substrate metabolism in cardiac hypertrophy or heart failure is a shift from fatty acid oxidation to glycolysis. The correlation between glycolysis and fatty acid oxidation and the underlying mechanism of cardiac pathological remodeling are not clear. We confirm that KLF7 targets both the rate-limiting enzyme of glycolysis, phosphofructokinase-1, liver, and the key enzyme for fatty acid oxidation, long-chain acyl-CoA dehydrogenase. The KLF7/PFKL/ACADL axis is a critical regulatory mechanism that may provide insight into therapeutic approaches for modulating cardiac metabolic balance in hypertrophied and failing heart.
NATURE COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Chrishan J. A. Ramachandra, Myu Mai Ja Kp, Jasper Chua, Sauri Hernandez-Resendiz, Elisa A. Liehn, Ralph Knoll, Li-Ming Gan, Erik Michaelsson, Malin K. B. Jonsson, Katarina Ryden-Markinhuhta, Ratan Bhat, Regina Fritsche-Danielson, Ying-Hsi Lin, Sakthivel Sadayappan, Hak Chiaw Tang, Philip Wong, Winston Shim, Derek J. Hausenloy
Summary: This study demonstrates that MPO inhibition can alleviate the relaxation defect in hypertrophic iPSC-CMs through MYBPC3 phosphorylation, highlighting cardiomyocyte MPO as a novel therapeutic target for improving myocardial relaxation associated with HCM.
CARDIOVASCULAR RESEARCH
(2022)
Article
Infectious Diseases
Francois Dolveck, Alessio Strazzulla, Coralie Noel, Sylvie Aufaure, Paul Tarteret, Astrid de Pontfarcy, Nicolas Briole, Nicolas Vignier, Sylvain Diamantis
Summary: An urgent pre-hospital intervention in eight French nursing homes resulted in a significant decrease in deaths and new COVID-19 cases, demonstrating the effectiveness of a multidisciplinary task force during the pandemic outbreak. The intervention included daily notifications of deaths, audits by infectious diseases and hygiene specialists, and emergency procedures performed by an emergency team. The reduction in mortality highlights the potential of pre-hospital interventions as an alternative to hospitalization during times of healthcare system saturation.
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Pathology
Alexandre Alanio, Maud Gits-Muselli, Fanny Lanternier, Aude Sturny-Leclere, Marion Benazra, Samia Hamane, Anderson Messias Rodrigues, Dea Garcia-Hermoso, Olivier Lortholary, Francoise Dromer, Stephane Bretagne
Summary: The laboratory diagnosis of histoplasmosis relies on various methods, such as microscopy, culture, antigen, and DNA detection. A new RT-qPCR assay was developed to amplify whole nucleic acids of Histoplasma spp., showing high sensitivity in diagnosing histoplasmosis from blood and bronchoalveolar lavage fluid. The study demonstrated the effectiveness of the assay in detecting Histoplasma capsulatum var. capsulatum and Histoplasma capsulatum var. duboisii infections with high specificity and sensitivity rates.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2021)
Review
Cell Biology
Saline Jabre, Walid Hleihel, Catherine Coirault
Summary: This review provides insights into the mechanisms of nuclear force transmission in skeletal muscle, emphasizing the critical role of nuclear mechanotransduction in muscle plasticity and discussing how it influences myogenic cell fate. It also suggests potential applications of these findings in treating muscular disorders and highlights current gaps in knowledge for further research in the field.
Article
Medicine, General & Internal
Mathieu Nacher, Mayka Mergeay-Fabre, Denis Blanchet, Orelie Benoit, Tristan Pozl, Pauline Mesphoule, Vincent Sainte-Rose, Veronique Vialette, Bruno Toulet, Aurelie Moua, Mona Saout, Stephane Simon, Manon Guidarelli, Muriel Galindo, Barbara Biche, William Faurous, Laurie Chaizemartin, Aniza Fahrasmane, Devi Rochemont, Nicolas Vignier, Astrid Vabret, Magalie Demar
Summary: Saliva testing for COVID-19 shows potential for use, especially in patients with low Ct values. Sensitivity is higher in patients with mild symptoms or sampled within 10 days of onset, while lower in asymptomatic patients.
FRONTIERS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Leticia Bertuzzi, Tarik El Aarbaoui, Megane Heron, Anne Gosselin, Laurine Roy-de-Lachaise, Larissa Fossi, Francesco Della Corte, Nicolas Vignier, Maria Melchior, Merritt Schreiber, Stephanie Vandentorren, Cecile Vuillermoz
Summary: This study aims to assess the mental health of healthcare professionals working with COVID-19 patients and identify social determinants that may increase the risk of negative outcomes. It also tests the effectiveness of an intervention to improve healthcare professionals' resilience in France.
Article
Public, Environmental & Occupational Health
Helene Duplan, Sebastien Rabier, Christine Sudre, Leila Adriouch, Aude Lucarelli, Florence Huber, Louise Mutricy, Jean Marc Wojcik, Nicolas Vignier, Etienne Pascolini, Antoine Adenis, Mathieu Nacher
Summary: Although hospital outpatient care is still the most common mode of patient care for HIV patients, there is a trend towards an increasing number of patients being exclusively followed by private practitioners in French Guiana.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Microbiology
Mathieu Nacher, Kinan Drak Alsibai, Loic Epelboin, Philippe Abboud, Fredegonde About, Magalie Demar, Felix Djossou, Romain Blaizot, Maylis Douine, Nadia Sabbah, Nicolas Vignier, Leila Adriouch, Aude Lucarelli, Mathilde Boutrou, Pierre Couppie, Antoine Adenis
Summary: This study conducted a retrospective analysis in French Guiana to compare disseminated histoplasmosis and tuberculosis, and constructed a predictive model to differentiate these two diseases.
Article
Microbiology
Alice Raffetin, Julien Schemoul, Amal Chahour, Steve Nguala, Pauline Caraux-Paz, Giulia Paoletti, Anna Belkacem, Fernanda Medina, Catherine Fabre, Sebastien Gallien, Nicolas Vignier, Yoann Madec
Summary: By comparing the clinical features of patients with different diagnoses and identifying factors associated with recovery, our study found that a multidisciplinary team dedicated to suspicion of LB can provide more accurate diagnosis and better medical support, leading to clinical improvement and avoiding inappropriate antibiotic prescription.
Review
Microbiology
Olivier Paccoud, Nicolas Vignier, Mohammed Boui, Melanie Migaud, Pierre Vironneau, Romain Kania, Frederic Mechai, Sophie Brun, Alexandre Alanio, Arnault Tauziede-Espariat, Homa Adle-Biassette, Elise Ouedraogo, Jacinta Bustamante, Olivier Bouchaud, Jean-Laurent Casanova, Anne Puel, Fanny Lanternier
Summary: Phaeohyphomycoses are a group of fungal infections caused by dematiaceous fungi and primarily occur in immunodeficient patients. This study reports a case of invasive rhinosinusitis caused by Alternaria infectoria in a previously healthy woman with CARD9 deficiency. In addition, 17 other cases of phaeohyphomycosis associated with CARD9 deficiency were reviewed.
Article
Chemistry, Multidisciplinary
Muhammad Haseeb Iqbal, Faratiana Jeanne Rosine Revana, Emeline Pradel, Varvara Gribova, Kamel Mamchaoui, Catherine Coirault, Florent Meyer, Fouzia Boulmedais
Summary: The engineering of skeletal muscle tissue is a promising approach for the treatment of muscle injuries and diseases. In this study, a hydrogen-bonded nanofilm was developed using a brushing method to promote muscle tissue development. The nanofilm successfully guided the alignment and differentiation of muscle cells, resulting in the formation of aligned myofibers.
Article
Cell Biology
Mark R. Viggars, Daniel J. Owens, Claire Stewart, Catherine Coirault, Abigail L. Mackey, Jonathan C. Jarvis
Summary: PCM1 is detected in single isolated myofibers and cross sections from mice and humans, playing an important role in the subcellular location of myonuclei. It is found in the nuclear envelope of mature myofibers and newly formed myotubes, as well as in the centrosomes of proliferating myogenic precursors. Although not completely specific to myonuclei, PCM1 may be useful as a marker of satellite cell dynamics.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Gabriel Brun-Rambaud, Leslie Alcouffe, Marc-Alexandre Tareau, Antoine Adenis, Nicolas Vignier
Summary: This study explores the barriers and facilitators to accessing health care for migrants in French Guiana in 2022. The research finds that migrants face various obstacles such as administrative complexity, language barriers, financial barriers, and mobility issues. In such situations, associative support and health mediation play a crucial role in providing close support.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Infectious Diseases
F. Mechai, D. Bachelet, L. Han, M. Dubert, M. Parisey, H. Cordel, A. Bourgarit, C. Bertrac, S. Chauveau, T. Billard-Pomares, E. Carbonnelle, O. Bouchaud, Y. Yazdanpanah, N. Vignier, C. Laouenan
Summary: This study aims to assess tuberculosis treatment outcomes and its determinants in the region of highest incidence in France. It found that socioeconomic conditions were not associated with treatment outcomes, while history of tuberculosis and young age played a role. Increased monitoring is required for these patients.
INFECTIOUS DISEASES NOW
(2022)
Letter
Infectious Diseases
P. Pugliese, C. Arvieux, T. Huleux, G. Pialoux, N. Vignier
INFECTIOUS DISEASES NOW
(2022)
Article
Infectious Diseases
N. Vignier, V. Halley des Fontaines, A. Billette de Villemeur, F. Cazenave-Roblot, B. Hoen, F. Chauvin, D. Lepelletier, C. Chidiac, E. Billaud
Summary: Given the increase in the number of people leaving the war zone in Ukraine and arriving in France, the French high council for public health has recommended prioritizing initial reception, implementing vaccination measures, and providing medical support.
INFECTIOUS DISEASES NOW
(2022)
