Article
Biochemistry & Molecular Biology
Akira Naito, Izuru Kawamura
Summary: Glucagon, a peptide hormone secreted by pancreatic alpha-cells, interacts with specific receptors in various organs. It can form cytotoxic gel-like fibril aggregates. Understanding the interaction of glucagon with cell membranes is crucial for studying its activity and cytotoxic effects.
BIOPHYSICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Oxana V. Galzitskaya, Olga M. Selivanova, Elena Y. Gorbunova, Leila G. Mustaeva, Viacheslav N. Azev, Alexey K. Surin
Summary: Under certain conditions, proteins and peptides can self-assemble into various supramolecular structures with potential pathological or functional implications. Fibrils, films, and amyloid gels are promising objects for nanobiotechnology research. Understanding the mechanism of structure formation and its influencing factors is crucial for developing nanobiomaterials with desired properties.
Article
Multidisciplinary Sciences
Shreya Ghosh, Vitali Tugarinov, G. Marius Clore
Summary: The chaperone Hsp104 prevents fibril formation of amyloidogenic peptides in a substochiometric manner. By studying its interaction with the A(342) peptide, it was found that Hsp104 binds reversibly to sparsely populated nuclei of A(342), completely inhibiting fibril formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Torsten John, Juliane Adler, Christian Elsner, Johannes Petzold, Martin Krueger, Lisandra L. Martin, Daniel Huster, Herre Jelger Risselada, Bernd Abel
Summary: The aggregation of peptides into amyloid fibrils is associated with ageing-related diseases. The size and curvature of nanoparticles can influence the kinetics of peptide aggregation, and different peptides may exhibit different responses to nanoparticles of different sizes. Experimental findings suggest that the surface binding affinity of nanoparticles and the physical properties of peptides play a role in peptide aggregation. Additional experiments support the size-dependent effects of nanoparticles on peptide aggregation and suggest a structural influence on the formed fibrils.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jacob Fritzsch, Alexander Korn, Dayana Surendran, Martin Krueger, Holger A. Scheidt, Kaustubh R. Mote, Perunthiruthy K. Madhu, Sudipta Maiti, Daniel Huster
Summary: Amyloid beta (A beta) is a peptide known to form amyloid fibrils in the brain of Alzheimer's disease patients. Mutations at positions Phe(20) or Gly(33) can lead to alterations in the fibrillation kinetics of A beta, as well as a severe decrease in cell toxicity compared to wildtype A beta(40). The region around the hydrophobic contact between Phe(19) and Leu(34) provides a promising site for designing small molecules to interfere with the A beta fibrillation pathway.
Article
Multidisciplinary Sciences
Daniel Horvath, Zsolt Durvanger, Dora K. Menyhard, Mate Sulyok-Eiler, Fruzsina Bencs, Gergo Gyulai, Peter Horvath, Nora Taricska, Andras Perczel
Summary: In this study, the authors reveal the dual nature of conserved receptor binding segments in class B GPCR ligands, which can undergo a pH-dependent conversion between secretory amyloid fibrils and functional folded form. They identify a hexapeptide sequence, xFxxWL, as the major aggregation-prone region (APR) in gastrointestinal peptides of the glucagon family. Through crystal structures and various assays, they propose that the protonation states of glutamate/aspartate residues in APRs play a crucial role in switching between amyloid and folded forms. Their findings emphasize the importance of short aggregation core motifs in reversible amyloid formation and receptor binding.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yui Nakata, Yuuto Kitazaki, Hitomi Kanaoka, Erika Shingen, Rina Uehara, Kunihiro Hongo, Yasushi Kawata, Tomohiro Mizobata
Summary: The molecular chaperones HdeA and HdeB help protect client proteins from acid denaturation in E. coli. Both proteins can form fibrils at low pH, but the fibrils can be resolubilized at neutral pH. However, extended incubation at low pH can lead to the formation of irreversible fibrils. The secondary structure of HdeB fibrils remained alpha helical regardless of the conditions under which the fibrils were formed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
You-Ren Lai, Tzu-Horng Wang, Su-Chun How, Kuen-Song Lin, Wei-Long Chou, Steven S-S Wang
Summary: This study reveals that sugar-terminated nanoparticles can effectively inhibit amyloid fibril formation and reduce aggregation levels. The binding between the nanoparticles and amyloid fibrils is primarily governed by hydrophobic interactions.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Biochemistry & Molecular Biology
Masafumi Tanaka, Toru Takarada, Satomi Nadanaka, Risa Kojima, Kimiko Hosoi, Yuki Machiba, Hiroshi Kitagawa, Toshiyuki Yamada
Summary: In this study, the effects of N-terminal modifications on amyloid fibril formation were investigated using a peptide corresponding to the N-terminal region of the SAA molecule. It was found that carbamoylation of the N-terminal amino group delayed the formation of amyloid fibrils, while acetylation or truncation of the N-terminal amino acids diminished the amyloidogenic properties. Cell toxicity assays showed that SAA peptides reduced cell reductive activity, but did not cause release of LDH. N-terminal truncation had no effect on cell activity or LDH release. These findings suggest that N-terminal modifications of SAA molecules can regulate susceptibility to AA amyloidosis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Soumav Nath, Priti Roy, Raki Mandal, Rajat Roy, Alexander K. Buell, Neelanjana Sengupta, Pradip K. Tarafdar
Summary: The study demonstrates the potential anti-amyloidogenic properties of endogenously derived compounds based on porphyrin, which can inhibit fibril formation of insulin and hen egg white lysozyme. Hematoporphyrin, derived from heme by hydroxylation and metal removal, shows superior inhibitory effects on insulin fibril formation compared to hemin and protoporphyrin.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Chemistry, Physical
Monika Holubova, Volodymyr Lobaz, Lenka Loukotova, Mariia Rabyk, Jirina Hromadkova, Olga Trhlikova, Zdislava Pechrova, Ondrej Groborz, Petr Stepanek, Martin Hruby
Summary: This study investigated the influence of various polysaccharides on amyloid fibril formation and found that all polysaccharides accelerated the process. At high concentrations, glycogen significantly accelerated amyloid fibril formation in HEWL. This suggests that dietary glucose intake may affect amyloid fibril formation through direct glycogen-amyloid precursor protein interaction.
Article
Biochemistry & Molecular Biology
Yoshito Abe, Hinako Shibata, Kousuke Oyama, Tadashi Ueda
Summary: This study expressed a V lambda 6 light chain mutant, Wil, by the yeast Pichia pastoris and found that glycosylation retarded amyloid fibril formation in a sugar size-dependent manner. The role of covalently attached glycan in the retardation of amyloid fibril formation was discussed based on the results.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Carlos Noble Jesus, Rhys Evans, Joe Forth, Carolina Estarellas, Francesco Luigi Gervasio, Giuseppe Battaglia
Summary: The study presents the design, simulation, synthesis, and reversible self-assembly of nanofibrils using polyhistidine-based oligopeptides. The inclusion of aromatic amino acids in the histidine block leads to the formation of amyloid-like fibrils with distinct antiparallel beta-strands. The structures undergo self-assembly in response to pH changes, offering potential for biotechnological and biomedical applications with pH-responsive fibrils in a physiologically relevant range.
Article
Chemistry, Medicinal
Angelo Santoro, Manuela Grimaldi, Michela Buonocore, Ilaria Stillitano, Anna Maria D'Ursi
Summary: This study utilized NMR to analyze the conformational transition of A beta(1-42) in 50/50 HFIP/water, revealing unexpected routes in the evolution from helical to beta-sheet structures. Molecular dynamics simulations confirmed that the structural model calculated in this study is a starting point for amyloid fibrils formation.
Article
Chemistry, Multidisciplinary
Yijia Guan, Dongqin Yu, Hanjun Sun, Jinsong Ren, Xiaogang Qu
Summary: The hydrophobic surface of the PPy/TCA film plays a significant role in adsorption and aggregation of A beta 40, rather than the hydrophilic surface.
CHEMICAL COMMUNICATIONS
(2021)
Article
Fisheries
Benni Winding Hansen, Giorgia Ciappini, Anders Malmendal, Thomas Allan Rayner
Summary: The copepod Apocyclops royi can survive and reproduce in freshwater for a period of time, but eventually stops reproducing due to the lack of vital ions. Metabolomics analysis showed significant changes in metabolites, with a large decrease in glycine betaine and a large increase in lactate in copepods challenged by freshwater. This study highlights the challenges and potential benefits of culturing A. royi in freshwater, as well as the possibility of developing a 'universal' live-feed product.
AQUACULTURE RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Hossein Mohammad-Beigi, Masumeh Zanganeh, Carsten Scavenius, Hoda Eskandari, Azad Farzadfard, Seyed Abbas Shojaosadati, Jan J. Enghild, Daniel E. Otzen, Alexander K. Buell, Duncan S. Sutherland
Summary: Nanoparticles can modulate protein aggregation and fibril formation in amyloid diseases. By attaching a protein corona to the nanoparticles, the interactions between alpha-Synuclein and the nanoparticles were studied. The results showed that the nanoparticles surface promotes the primary nucleation step of fibril formation and that the protein corona reduces the accelerating effect of the nanoparticles.
Article
Chemistry, Physical
Maroua Ben Haj Salah, Nicolas Mercier, Magali Allain, Antonin Leblanc, Jens Dittmer, Chiara Botta, Claudio Quarti, Claudine Katan
Summary: Metal-halide perovskites, particularly the layered subfamily, have shown great potential for optoelectronic applications. In this study, a <1 1 0>-oriented (FA)(3)(HEA)(2)Pb3I11 perovskite was successfully synthesized and characterized. The perovskite exhibited a broad absorption band in the visible region and a direct band gap, suggesting its potential for photovoltaic applications.
CHEMISTRY OF MATERIALS
(2022)
Article
Chemistry, Inorganic & Nuclear
Cyrille Galven, Maxime Ducamp, Xavier Rocquefelte, Jens Dittmer, Marie-Pierre Crosnier-Lopez, Francoise Le Berre
Summary: A new oxygen-deficient perovskite Ba3LiNb2O8.5 square(0.5) was synthesized and its thermal behavior and color change were investigated. The results suggest that both Nb/Li distribution and oxygen-vacancy location play important roles in the properties of this perovskite.
INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tinna Palmadottir, Christopher A. Waudby, Katja Bernfur, John Christodoulou, Sara Linse, Anders Malmendal
Summary: In this study, two morphologically distinct α-synuclein fibrils were observed under identical conditions. The two fibrils, A and B, were found to have different surface properties and solubility. Over time, fibrils of morphology A gradually transformed into morphology B.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Christian E. Rusbjerg-Weberskov, Mette Liere Johansen, Jan S. Nowak, Daniel E. Otzen, Jan Skov Pedersen, Jan J. Enghild, Nadia Sukusu Nielsen
Summary: Human periostin is a matricellular protein that plays important roles in extracellular matrix remodeling, tumor development, and inflammatory diseases. The C-terminal domain of periostin interacts with other domains and proteins, potentially contributing to the pathogenesis of diseases such as atopic dermatitis.
Review
Chemistry, Physical
Marcos Lopez Hernandez, Jan Skov Pedersen, Daniel E. Otzen
Summary: The synergies between surfactants and proteins are widely utilized in various fields, but there is still room for improvement in terms of sustainability. Biosurfactants offer an attractive alternative, and understanding their interactions with proteins can open up new opportunities. This review focuses on the self-assembly of protein:BS complexes and the effects of BS on enzymatic activity.
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
(2023)
Review
Chemistry, Analytical
Hashmatullah Nasimi, Jonna Skov Madsen, Ahmed H. Zedan, Anders Malmendal, Palle Jorn Sloth Osther, Fatima AlZahra'a Alatraktchi
Summary: Amino acids such as tyrosine and tryptophan have attracted attention as potential biomarkers for various diseases. Electrochemical sensors offer a simple, cost-effective, and highly sensitive alternative to metabolomic methods for detecting these amino acids.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Steven J. Roeters, Kris Strunge, Kasper B. Pedersen, Thaddeus W. Golbek, Mikkel Bregnhoj, Yuge Zhang, Yin Wang, Mingdong Dong, Janni Nielsen, Daniel E. Otzen, Birgit Schiott, Tobias Weidner
Summary: This study reports a mechanism explaining why lipid membranes catalyze the formation of harmful aggregates at elevated concentrations.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Hashmatullah Nasimi, Jonna Skov Madsen, Ahmed H. H. Zedan, Anders Malmendal, Palle Jorn Sloth Osther, Fatima AlZahra'a Alatraktchi
Summary: By using electrochemical biosensing, the detection of β-2-Microglobulin (β2M) in urine is introduced as a potential diagnostic tool for prostate cancer (PCa). The sensor is capable of directly detecting the protein in urine without any sample pretreatment within 45 min, with a lower limit of detection of 204 μg/L. This electrochemical sensing approach may pave the way for an affordable, on-site PCa screening technique.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Multidisciplinary
Morten K. Sorensen, Anne L. Hansen, Hendrik Jan C. van Dooren, Nico W. M. Ogink, Ole Aaes, Ole N. Jensen, Niels Chr. Nielsen, Anders Feilberg, Peter Kai
Summary: In order to reduce environmental pollution caused by nitrogen (N) emissions and achieve green transition of modern agriculture and sustainable agricultural expansion to meet global food demands, we propose a sensor-based mass-balance model that utilizes the ratio between N and an internal tracer (such as phosphorus [P], potassium [K], or sodium [Na]) driven by on-site NMR slurry measurements to address the challenging gas emission measurements in naturally ventilated livestock housings. Through a full-scale barn experiment, we found that sodium (Na) is a suitable tracer and an N/Na-based mass-balance model can provide valuable measurements of N emissions in naturally ventilated barns when extensive, careful sampling, and accurate analysis are performed using mobile NMR sensor technology.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Hashmatullah Nasimi, Jonna Skov Madsen, Ahmed H. Zedan, Anders Malmendal, Palle Jorn Sloth Osther, Fatima AlZahra'a Alatraktchi
Summary: Rapid on-site electrochemical screening has the potential for early diagnosis of prostate cancer. Commonly used biomarkers currently lack accuracy, but several protein biomarker candidates show promise for diagnostic or prognostic screening. This review focuses on the advancements in electrochemical biosensor development specifically targeting the detection of these biomarkers. It emphasizes the importance of using multiplex electrochemical methods to measure panels of protein biomarkers for more precise diagnostic information.
SENSORS AND ACTUATORS REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Egle Kelpsiene, Tommy Cedervall, Anders Malmendal
Summary: Larger plastic pieces break down into micro- and eventually nano-sized plastics, making nanoplastics ubiquitous in the environment and raising concerns about its impact on biota. This study used H-1 nuclear magnetic resonance (NMR) metabolomics to investigate the metabolic changes in Daphnia magna exposed to low concentrations of positively and negatively charged polystyrene nanoparticles (PS-NPs). The results showed that exposure to PS-NPs affected amino acid metabolism and bacterial metabolite isopropanol in D. magna, independent of particle concentration and surface charge.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Endocrinology & Metabolism
Rasmus Bo Hasselbalch, Jonas Henrik Kristensen, Nina Strandkjaer, Nicoline Jorgensen, Henning Bundgaard, Anders Malmendal, Kasper Karmark Iversen
Summary: In this study, changes in metabolites after induced ischemia were investigated. It was found that ischemia led to early changes in lipid metabolism, especially in high-density lipoprotein.
Article
Chemistry, Inorganic & Nuclear
Cyrille Galven, Maxime Ducamp, Xavier Rocquefelte, Jens Dittmer, Marie-Pierre Crosnier-Lopez, Francoise Le Berre
Summary: The structure of Ba3LiNb2O8.5 square(0.5) was investigated using various techniques, revealing unexpected color change at high temperatures while maintaining structural integrity. First-principles calculations highlighted the importance of Nb/Li distribution and oxygen-vacancy location.
INORGANIC CHEMISTRY
(2022)